ID TIM44_MOUSE Reviewed; 452 AA. AC O35857; DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot. DT 01-JAN-1998, sequence version 1. DT 08-MAR-2011, entry version 77. DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM44; DE Flags: Precursor; GN Name=Timm44; Synonyms=Mimt44, Tim44; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=CD-1; TISSUE=Kidney; RX MEDLINE=98081841; PubMed=9419343; DOI=10.1073/pnas.95.1.144; RA Wada J., Kanwar Y.S.; RT "Characterization of mammalian translocase of inner mitochondrial RT membrane (Tim44) isolated from diabetic newborn mouse kidney."; RL Proc. Natl. Acad. Sci. U.S.A. 95:144-149(1998). CC -!- FUNCTION: Essential component of the PAM complex, a complex CC required for the translocation of transit peptide-containing CC proteins from the inner membrane into the mitochondrial matrix in CC an ATP-dependent manner. Recruits mitochondrial HSP70 to drive CC protein translocation into the matrix using ATP as an energy CC source. CC -!- SUBUNIT: Probable component of the PAM complex at least composed CC of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, CC TIMM16/PAM16 and TIMM14/DNAJC19. The complex interacts with the CC TIMM23 component of the TIM23 complex (By similarity). CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane. CC -!- SIMILARITY: Belongs to the Tim44 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U69898; AAB97624.1; -; mRNA. DR IPI; IPI00135068; -. DR UniGene; Mm.195249; -. DR ProteinModelPortal; O35857; -. DR SMR; O35857; 270-451. DR STRING; O35857; -. DR REPRODUCTION-2DPAGE; O35857; -. DR PRIDE; O35857; -. DR Ensembl; ENSMUST00000003029; ENSMUSP00000003029; ENSMUSG00000002949. DR UCSC; uc009ktq.1; mouse. DR MGI; MGI:1343262; Timm44. DR HOGENOM; HBG378129; -. DR HOVERGEN; HBG055113; -. DR InParanoid; O35857; -. DR OrthoDB; EOG49CQ7J; -. DR PhylomeDB; O35857; -. DR ArrayExpress; O35857; -. DR Bgee; O35857; -. DR CleanEx; MM_TIMM44; -. DR Genevestigator; O35857; -. DR GermOnline; ENSMUSG00000002949; Mus musculus. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR InterPro; IPR017303; Tim44. DR InterPro; IPR007379; Tim44-related/Ribosome_L45. DR Pfam; PF04280; Tim44; 1. DR PIRSF; PIRSF037871; TIM44; 1. DR SMART; SM00978; Tim44; 1. DR TIGRFAMs; TIGR00984; 3a0801s03tim44; 1. PE 2: Evidence at transcript level; KW ATP-binding; Membrane; Mitochondrion; Mitochondrion inner membrane; KW Nucleotide-binding; Protein transport; Transit peptide; Translocation; KW Transport. FT TRANSIT 1 ? Mitochondrion (Potential). FT CHAIN ? 452 Mitochondrial import inner membrane FT translocase subunit TIM44. FT /FTId=PRO_0000034315. FT NP_BIND 166 173 ATP (Potential). SQ SEQUENCE 452 AA; 51176 MW; 18E2F61356F7B9F4 CRC64; MAAARLRGGW CRCPRRCLGS GIQFLSSHNL PHGSSYQISR PGRELTLTKS YSSGSRKGFL SGLLDNIKQE LAKNKEMKES IKKFRDEAKK LEESDALQEA RRKYKSIESE TVRTSEAIKK KLGELTGTVK ESLDEVSKSD LGRKIKEGVE EAARTAKQSA ESVSKSGEKL GKTAAFKAIS QGVESVKKEL DESVLGQTGP YRRPERLRKR TEFAGAKFKE SKVFEANEEA LGVVLHKDSK WYQQWKDFKD NNVVFNRFFE MKMKYDESDN VLIRASRALT DKVTDLLGGL FSKTEMSEVL TEILRVDPTF DKDHFLHQCE TDIIPNILEA MISGELDILK DWCYEATYSQ LAHPIQQAKA LGFQFHSRIL DISNVDLAMG KMMEQGPVLI VTFQAQVVMV IKNSKGEVYD GDPDKVQRML YVWALCRDQE ELNPYAAWRL LDISASSTEQ IL //