ID TIM44_MOUSE Reviewed; 452 AA. AC O35857; Q2NLC5; DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot. DT 27-JUL-2011, sequence version 2. DT 03-SEP-2014, entry version 103. DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM44; DE Flags: Precursor; GN Name=Timm44; Synonyms=Mimt44, Tim44; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=CD-1; TISSUE=Kidney; RX PubMed=9419343; DOI=10.1073/pnas.95.1.144; RA Wada J., Kanwar Y.S.; RT "Characterization of mammalian translocase of inner mitochondrial RT membrane (Tim44) isolated from diabetic newborn mouse kidney."; RL Proc. Natl. Acad. Sci. U.S.A. 95:144-149(1998). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.; RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=NMRI; TISSUE=Mammary tumor; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [4] RP SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-177 AND LYS-217, AND RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Liver; RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001; RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., RA Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.; RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic RT pathways."; RL Mol. Cell 50:919-930(2013). CC -!- FUNCTION: Essential component of the PAM complex, a complex CC required for the translocation of transit peptide-containing CC proteins from the inner membrane into the mitochondrial matrix in CC an ATP-dependent manner. Recruits mitochondrial HSP70 to drive CC protein translocation into the matrix using ATP as an energy CC source. CC -!- SUBUNIT: Probable component of the PAM complex at least composed CC of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, CC TIMM16/PAM16 and TIMM14/DNAJC19. The complex interacts with the CC TIMM23 component of the TIM23 complex (By similarity). CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane. CC -!- SIMILARITY: Belongs to the Tim44 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U69898; AAB97624.1; -; mRNA. DR EMBL; CH466566; EDL21992.1; -; Genomic_DNA. DR EMBL; BC110677; AAI10678.1; -; mRNA. DR EMBL; BC117523; AAI17524.1; -; mRNA. DR EMBL; BC117524; AAI17525.1; -; mRNA. DR CCDS; CCDS22083.1; -. DR RefSeq; NP_035722.2; NM_011592.2. DR UniGene; Mm.195249; -. DR ProteinModelPortal; O35857; -. DR SMR; O35857; 270-451. DR MINT; MINT-1845288; -. DR STRING; 10090.ENSMUSP00000003029; -. DR PhosphoSite; O35857; -. DR REPRODUCTION-2DPAGE; O35857; -. DR MaxQB; O35857; -. DR PaxDb; O35857; -. DR PRIDE; O35857; -. DR Ensembl; ENSMUST00000003029; ENSMUSP00000003029; ENSMUSG00000002949. DR GeneID; 21856; -. DR KEGG; mmu:21856; -. DR UCSC; uc009ktq.2; mouse. DR CTD; 10469; -. DR MGI; MGI:1343262; Timm44. DR eggNOG; NOG240138; -. DR GeneTree; ENSGT00390000000051; -. DR HOGENOM; HOG000013004; -. DR HOVERGEN; HBG055113; -. DR InParanoid; Q2NLC5; -. DR KO; K17804; -. DR OMA; KQCENDI; -. DR OrthoDB; EOG7H792G; -. DR TreeFam; TF106197; -. DR Reactome; REACT_199101; Mitochondrial protein import. DR ChiTaRS; TIMM44; mouse. DR NextBio; 301346; -. DR PRO; PR:O35857; -. DR ArrayExpress; O35857; -. DR Bgee; O35857; -. DR CleanEx; MM_TIMM44; -. DR Genevestigator; O35857; -. DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:MGI. DR GO; GO:0005759; C:mitochondrial matrix; IEA:Ensembl. DR GO; GO:0005739; C:mitochondrion; IDA:MGI. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0015450; F:P-P-bond-hydrolysis-driven protein transmembrane transporter activity; IEA:InterPro. DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro. DR InterPro; IPR017303; Tim44. DR InterPro; IPR007379; Tim44-like_dom. DR Pfam; PF04280; Tim44; 1. DR PIRSF; PIRSF037871; TIM44; 1. DR SMART; SM00978; Tim44; 1. DR TIGRFAMs; TIGR00984; 3a0801s03tim44; 1. PE 1: Evidence at protein level; KW ATP-binding; Complete proteome; Membrane; Mitochondrion; KW Mitochondrion inner membrane; Nucleotide-binding; Phosphoprotein; KW Protein transport; Reference proteome; Transit peptide; Translocation; KW Transport. FT TRANSIT 1 ? Mitochondrion (Potential). FT CHAIN ? 452 Mitochondrial import inner membrane FT translocase subunit TIM44. FT /FTId=PRO_0000034315. FT NP_BIND 166 173 ATP (Potential). FT MOD_RES 177 177 N6-succinyllysine. FT MOD_RES 180 180 Phosphoserine (By similarity). FT MOD_RES 217 217 N6-succinyllysine. FT CONFLICT 5 5 A -> R (in Ref. 1; AAB97624). SQ SEQUENCE 452 AA; 51091 MW; DC3E5F4E43972D2F CRC64; MAAAALRGGW CRCPRRCLGS GIQFLSSHNL PHGSSYQISR PGRELTLTKS YSSGSRKGFL SGLLDNIKQE LAKNKEMKES IKKFRDEAKK LEESDALQEA RRKYKSIESE TVRTSEAIKK KLGELTGTVK ESLDEVSKSD LGRKIKEGVE EAARTAKQSA ESVSKSGEKL GKTAAFKAIS QGVESVKKEL DESVLGQTGP YRRPERLRKR TEFAGAKFKE SKVFEANEEA LGVVLHKDSK WYQQWKDFKD NNVVFNRFFE MKMKYDESDN VLIRASRALT DKVTDLLGGL FSKTEMSEVL TEILRVDPTF DKDHFLHQCE TDIIPNILEA MISGELDILK DWCYEATYSQ LAHPIQQAKA LGFQFHSRIL DISNVDLAMG KMMEQGPVLI VTFQAQVVMV IKNSKGEVYD GDPDKVQRML YVWALCRDQE ELNPYAAWRL LDISASSTEQ IL //