ID CBIT_METTH STANDARD; PRT; 192 AA.
AC O26249;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 04-APR-2006, entry version 39.
DE Probable cobalt-precorrin-6Y C(15)-methyltransferase [decarboxylating]
DE (EC 2.1.1.-).
GN Name=cbiT; OrderedLocusNames=MTH146;
OS Methanobacterium thermoautotrophicum.
OC Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales;
OC Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Delta H;
RX MEDLINE=98037514; PubMed=9371463;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K.,
RA Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D.,
RA Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R.,
RA Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S.,
RA McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M.,
RA Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum
RT deltaH: functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
RX MEDLINE=22317553; PubMed=12429089; DOI=10.1016/S0969-2126(02)00876-6;
RA Keller J.P., Smith P.M., Benach J., Christendat D., deTitta G.T.,
RA Hunt J.F.;
RT "The crystal structure of MT0146/CbiT suggests that the putative
RT precorrin-8w decarboxylase is a methyltransferase.";
RL Structure 10:1475-1487(2002).
CC -!- FUNCTION: Probably catalyzes the methylation of either C-15 or C-5
CC in cobalt-precorrin-6Y to form cobalt-precorrin-7W. Methylation of
CC C-15 would probably be followed by a spontaneous decarboxylation
CC of C-12.
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC cob(II)yrinic acid a,c-diamide from precorrin-2 (anaerobic route):
CC step 8.
CC -!- SUBUNIT: Homotetramer.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. Archeal-
CC type cbiT family.
CC -!- CAUTION: Was originally thought to be a precorrin-8w
CC decarboxylase.
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DR EMBL; AE000666; AAB84652.1; -; Genomic_DNA.
DR PIR; D69061; D69061.
DR PDB; 1F38; X-ray; A/B/C/D=1-192.
DR PDB; 1KXZ; X-ray; A/B/C/D/E/F/G/H=1-192.
DR PDB; 1L3B; X-ray; A/B/C/D/E/F/G/H=1-192.
DR PDB; 1L3C; X-ray; A/B/C/D=1-192.
DR PDB; 1L3I; X-ray; A/B/C/D/E/F=1-192.
DR GenomeReviews; AE000666_GR; MTH146.
DR BioCyc; MTHE187420:MTH146-MONOMER; -.
DR LinkHub; O26249; -.
DR HAMAP; MF_00786; -; 1.
DR InterPro; IPR013216; Methyltransf_11.
DR InterPro; IPR012376; Precrr_mtas_CbiT.
DR InterPro; IPR000051; SAM_bd.
DR Pfam; PF08241; Methyltransf_11; 1.
DR PIRSF; PIRSF019019; Precrr_mtas_CbiT; 1.
KW 3D-structure; Cobalamin biosynthesis; Complete proteome;
KW Methyltransferase; Transferase.
FT CHAIN 1 192 Probable cobalt-precorrin-6Y C(15)-
FT methyltransferase [decarboxylating].
FT /FTId=PRO_0000134941.
FT HELIX 4 6
FT STRAND 7 7
FT TURN 11 12
FT STRAND 13 13
FT HELIX 19 29
FT TURN 33 34
FT STRAND 36 41
FT TURN 43 44
FT STRAND 45 45
FT HELIX 46 52
FT TURN 53 54
FT STRAND 55 63
FT HELIX 65 77
FT TURN 78 79
FT TURN 82 83
FT STRAND 84 89
FT HELIX 91 95
FT TURN 96 97
FT STRAND 98 98
FT STRAND 101 107
FT TURN 110 111
FT HELIX 113 122
FT TURN 123 123
FT STRAND 124 134
FT STRAND 136 136
FT HELIX 137 149
FT TURN 150 151
FT STRAND 155 167
FT TURN 168 169
FT STRAND 170 175
FT STRAND 179 183
SQ SEQUENCE 192 AA; 20714 MW; 7BFA35D8BD3C3982 CRC64;
MIPDDEFIKN PSVPGPTAME VRCLIMCLAE PGKNDVAVDV GCGTGGVTLE LAGRVRRVYA
IDRNPEAIST TEMNLQRHGL GDNVTLMEGD APEALCKIPD IDIAVVGGSG GELQEILRII
KDKLKPGGRI IVTAILLETK FEAMECLRDL GFDVNITELN IARGRALDRG TMMVSRNPVA
LIYTGVSHEN KD
//