ID CBIT_METTH STANDARD; PRT; 192 AA. AC O26249; DT 05-JUL-2004 (Rel. 44, Created) DT 05-JUL-2004 (Rel. 44, Last sequence update) DT 01-OCT-2004 (Rel. 45, Last annotation update) DE Probable cobalt-precorrin-6Y C(15)-methyltransferase [decarboxylating] DE (EC 2.1.1.-). GN Name=cbiT; OrderedLocusNames=MTH146; OS Methanobacterium thermoautotrophicum. OC Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; OC Methanobacteriaceae; Methanothermobacter. OX NCBI_TaxID=187420; RN [1] RP SEQUENCE FROM N.A. RC STRAIN=Delta H; RX MEDLINE=98037514; PubMed=9371463; RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., RA Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., RA Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R., RA Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., RA McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., RA Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.; RT "Complete genome sequence of Methanobacterium thermoautotrophicum RT deltaH: functional analysis and comparative genomics."; RL J. Bacteriol. 179:7135-7155(1997). RN [2] RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS). RX PubMed=12429089; DOI=10.1016/S0969-2126(02)00876-6; RA Keller J.P., Smith P.M., Benach J., Christendat D., deTitta G.T., RA Hunt J.F.; RT "The crystal structure of MT0146/CbiT suggests that the putative RT precorrin-8w decarboxylase is a methyltransferase."; RL Structure 10:1475-1487(2002). CC -!- FUNCTION: Probably catalyzes the methylation of either C-15 or C-5 CC in cobalt-precorrin-6Y to form cobalt-precorrin-7W. Methylation of CC C-15 would probably be followed by a spontaneous decarboxylation CC of C-12. CC -!- PATHWAY: Adenosylcobalamin biosynthesis; anaerobic branch of CC corrin ring synthesis; tenth step. CC -!- SUBUNIT: Homotetramer. CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. Archeal- CC type cbiT family. CC -!- CAUTION: Was originally thought to be a precorrin-8w CC decarboxylase. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE000803; AAB84652.1; -. DR PIR; D69061; D69061. DR PDB; 1F38; X-ray; A/B/C/D=1-192. DR PDB; 1KXZ; X-ray; A/B/C/D/E/F/G/H=1-192. DR PDB; 1L3B; X-ray; A/B/C/D/E/F/G/H=1-192. DR PDB; 1L3C; X-ray; A/B/C/D=1-192. DR PDB; 1L3I; X-ray; A/B/C/D/E/F=1-192. DR HAMAP; MF_00786; -; 1. DR InterPro; IPR001000; Glyco_hydro_10. DR InterPro; IPR000051; SAM_bind. KW 3D-structure; Cobalamin biosynthesis; Complete proteome; KW Methyltransferase; Transferase. SQ SEQUENCE 192 AA; 20714 MW; 7BFA35D8BD3C3982 CRC64; MIPDDEFIKN PSVPGPTAME VRCLIMCLAE PGKNDVAVDV GCGTGGVTLE LAGRVRRVYA IDRNPEAIST TEMNLQRHGL GDNVTLMEGD APEALCKIPD IDIAVVGGSG GELQEILRII KDKLKPGGRI IVTAILLETK FEAMECLRDL GFDVNITELN IARGRALDRG TMMVSRNPVA LIYTGVSHEN KD //