ID   CBIT_METTH     STANDARD;      PRT;   192 AA.
AC   O26249;
DT   05-JUL-2004 (Rel. 44, Created)
DT   05-JUL-2004 (Rel. 44, Last sequence update)
DT   01-OCT-2004 (Rel. 45, Last annotation update)
DE   Probable cobalt-precorrin-6Y C(15)-methyltransferase [decarboxylating]
DE   (EC 2.1.1.-).
GN   Name=cbiT; OrderedLocusNames=MTH146;
OS   Methanobacterium thermoautotrophicum.
OC   Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales;
OC   Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   SEQUENCE FROM N.A.
RC   STRAIN=Delta H;
RX   MEDLINE=98037514; PubMed=9371463;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K.,
RA   Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D.,
RA   Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R.,
RA   Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S.,
RA   McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M.,
RA   Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum
RT   deltaH: functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
RX   PubMed=12429089;
RA   Keller J.P., Smith P.M., Benach J., Christendat D., deTitta G.T.,
RA   Hunt J.F.;
RT   "The crystal structure of MT0146/CbiT suggests that the putative
RT   precorrin-8w decarboxylase is a methyltransferase.";
RL   Structure 10:1475-1487(2002).
CC   -!- FUNCTION: Probably catalyzes the methylation of either C-15 or C-5
CC       in cobalt-precorrin-6Y to form cobalt-precorrin-7W. Methylation of
CC       C-15 would probably be followed by a spontaneous decarboxylation
CC       of C-12.
CC   -!- PATHWAY: Adenosylcobalamin biosynthesis; anaerobic branch of
CC       corrin ring synthesis; tenth step.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. Archeal-
CC       type cbiT family.
CC   -!- CAUTION: Was originally thought to be a precorrin-8w
CC       decarboxylase.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000803; AAB84652.1; -.
DR   PIR; D69061; D69061.
DR   PDB; 1F38; 10-JUN-03.
DR   PDB; 1KXZ; 27-NOV-02.
DR   PDB; 1L3B; 27-NOV-02.
DR   PDB; 1L3C; 27-NOV-02.
DR   PDB; 1L3I; 27-NOV-02.
DR   HAMAP; MF_00786; -; 1.
DR   InterPro; IPR001000; Glyco_hydro_10.
DR   InterPro; IPR000051; SAM_bind.
KW   3D-structure; Cobalamin biosynthesis; Complete proteome;
KW   Methyltransferase; Transferase.
SQ   SEQUENCE   192 AA;  20714 MW;  7BFA35D8BD3C3982 CRC64;
     MIPDDEFIKN PSVPGPTAME VRCLIMCLAE PGKNDVAVDV GCGTGGVTLE LAGRVRRVYA
     IDRNPEAIST TEMNLQRHGL GDNVTLMEGD APEALCKIPD IDIAVVGGSG GELQEILRII
     KDKLKPGGRI IVTAILLETK FEAMECLRDL GFDVNITELN IARGRALDRG TMMVSRNPVA
     LIYTGVSHEN KD
//