ID AOX2_ARATH STANDARD; PRT; 353 AA. AC O22049; DT 15-DEC-1998 (Rel. 37, Created) DT 28-FEB-2003 (Rel. 41, Last sequence update) DT 01-OCT-2004 (Rel. 45, Last annotation update) DE Alternative oxidase 2, mitochondrial precursor (EC 1.-.-.-). GN Name=AOX2; OrderedLocusNames=At5g64210; ORFNames=MSJ1.5; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicots; rosids; OC eurosids II; Brassicales; Brassicaceae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP SEQUENCE FROM N.A., SUBCELLULAR LOCATION, TISSUE SPECIFICITY, RP DEVELOPMENTAL STAGE, AND INDUCTION. RC STRAIN=cv. Columbia; RX MEDLINE=21327317; PubMed=11434463; RA Saisho D., Nakazono M., Lee K.-H., Tsutsumi N., Akita S., Hirai A.; RT "The gene for alternative oxidase-2 (AOX2) from Arabidopsis thaliana RT consists of five exons unlike other AOX genes and is transcribed at an RT early stage during germination."; RL Genes Genet. Syst. 76:89-97(2001). RN [2] RP SEQUENCE OF 72-353 FROM N.A. RC STRAIN=cv. Columbia; TISSUE=Leaf, and Stem; RX MEDLINE=98009993; PubMed=9349280; RA Saisho D., Nambara E., Naito S., Tsutsumi N., Hirai A., Nakazono M.; RT "Characterization of the gene family for alternative oxidase from RT Arabidopsis thaliana."; RL Plant Mol. Biol. 35:585-596(1997). RN [3] RP SEQUENCE FROM N.A. RC STRAIN=cv. Columbia; RX MEDLINE=98162728; PubMed=9501997; RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., RA Tabata S.; RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. RT Sequence features of the regions of 1,191,918 bp covered by seventeen RT physically assigned P1 clones."; RL DNA Res. 4:401-414(1997). RN [4] RP IRON BINDING SITES. RX MEDLINE=99239925; PubMed=10225419; RA Andersson M.E., Nordlund P.; RT "A revised model of the active site of alternative oxidase."; RL FEBS Lett. 449:17-22(1999). CC -!- FUNCTION: Catalyzes cyanide-resistant oxygen consumption. May CC increase respiration when the cytochrome respiratory pathway is CC restricted, or in response to low temperatures (By similarity). CC -!- COFACTOR: Binds two iron ions per subunit. CC -!- PATHWAY: Alternative respiratory pathway. CC -!- SUBUNIT: Homodimer; disulfide-linked (Probable). CC -!- SUBCELLULAR LOCATION: Mitochondrial, possibly in the inner surface CC of the inner mitochondrial membrane. CC -!- TISSUE SPECIFICITY: Maximally expressed in dry seeds. CC -!- DEVELOPMENTAL STAGE: Found predominantly during early stages of CC germination with maximum level at 12 hours after imbibition. CC -!- INDUCTION: Antimycin A has no effect on expression. CC -!- SIMILARITY: Belongs to the alternative oxidase family. CC -!- CAUTION: Ref.3 sequence differs from that shown due to erroneous CC gene model prediction. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB003176; BAA22636.2; -. DR EMBL; AB008268; BAB09852.1; ALT_SEQ. DR GeneFarm; 1754; 131. DR InterPro; IPR002680; AOX. DR Pfam; PF01786; AOX; 1. KW Inner membrane; Iron; Mitochondrion; Multigene family; Oxidoreductase; KW Respiratory chain; Transit peptide; Transmembrane. FT TRANSIT 1 21 Mitochondrion (Potential). FT CHAIN 22 353 Alternative oxidase 2. FT TRANSMEM 178 198 Potential. FT TRANSMEM 240 260 Potential. FT METAL 182 182 Iron (Potential). FT METAL 221 221 Iron (Potential). FT METAL 224 224 Iron (Potential). FT METAL 273 273 Iron (Potential). FT METAL 323 323 Iron (Potential). FT METAL 326 326 Iron (Potential). FT DISULFID 126 126 Interchain (Potential). SQ SEQUENCE 353 AA; 40086 MW; DF7ACB113E57A133 CRC64; MSQLITKAAL RVLLVCGRGN CNMFVSSVSS TSVMKSPYEI TAPMRIHDWC GGFGDFKIGS KHVQGNFNLR WMGMSSASAM EKKDENLTVK KGQNGGGSVA VPSYWGIETA KMKITRKDGS DWPWNCFMPW ETYQANLSID LKKHHVPKNI ADKVAYRIVK LLRIPTDIFF QRRYGCRAMM LETVAAVPGM VGGMLLHLKS IRKFEHSGGW IKALLEEAEN ERMHLMTMME LVKPKWYERL LVMLVQGIFF NSFFVCYVIS PRLAHRVVGY LEEEAIHSYT EFLKDIDNGK IENVAAPAIA IDYWRLPKDA TLKDVVTVIR ADEAHHRDVN HFASDIRNQG KELREAAAPI GYH //