ID M3K1_ARATH STANDARD; PRT; 666 AA. AC O22040; O22039; Q8GZ05; DT 05-JUL-2004 (Rel. 44, Created) DT 05-JUL-2004 (Rel. 44, Last sequence update) DT 25-JAN-2005 (Rel. 46, Last annotation update) DE Mitogen-activated protein kinase kinase kinase 1 (EC 2.7.1.-) DE (Arabidospsis NPK1-related protein kinase 1). GN Name=ANP1; OrderedLocusNames=At1g09000; ORFNames=F7G19.13; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicots; rosids; OC eurosids II; Brassicales; Brassicaceae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP SEQUENCE FROM N.A. (ISOFORMS 1S AND 1L), AND TISSUE SPECIFICITY. RC STRAIN=cv. Columbia; RX MEDLINE=97408932; PubMed=9263451; RA Nishihama R., Banno H., Kawahara E., Irie K., Machida Y.; RT "Possible involvement of differential splicing in regulation of the RT activity of Arabidopsis ANP1 that is related to mitogen-activated RT protein kinase kinase kinases (MAPKKKs)."; RL Plant J. 12:39-48(1997). RN [2] RP SEQUENCE FROM N.A. RC STRAIN=cv. Columbia; RX MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500; RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., RA White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., RA Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., RA Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., RA Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., RA Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., RA Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., RA Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., RA Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., RA Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., RA Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., RA Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., RA Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., RA Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., RA Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.; RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis RT thaliana."; RL Nature 408:816-820(2000). RN [3] RP SEQUENCE FROM N.A. (ISOFORM 1L). RC STRAIN=cv. Columbia; RX PubMed=11910074; DOI=10.1126/science.1071006; RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., RA Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., RA Shibata K., Shinagawa A., Shinozaki K.; RT "Functional annotation of a full-length Arabidopsis cDNA collection."; RL Science 296:141-145(2002). RN [4] RP SEQUENCE FROM N.A. (ISOFORM 1L). RC STRAIN=cv. Columbia; RX MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1L; CC IsoId=O22040-1; Sequence=Displayed; CC Name=1S; CC IsoId=O22040-2; Sequence=VSP_010124, VSP_010125; CC -!- TISSUE SPECIFICITY: Expressed in roots, inflorescence stems, CC flower buds and flowers. Low amount in rosette and cauline leaves. CC -!- MISCELLANEOUS: The protein kinase activity of isoform 1S is higher CC than that of isoform 1L. CC -!- SIMILARITY: Belongs to the Ser/Thr protein kinase family. MAP CC kinase kinase kinase subfamily. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB000796; BAA21854.1; -. DR EMBL; AB000797; BAA21855.1; -. DR EMBL; AK117282; BAC41954.1; -. DR EMBL; BT005949; AAO64884.1; -. DR HSSP; P24941; 1AQ1. DR GeneFarm; 898; 89. DR InterPro; IPR000719; Prot_kinase. DR InterPro; IPR008271; Ser_thr_pkin_AS. DR InterPro; IPR002290; Ser_thr_pkinase. DR InterPro; IPR001245; Tyr_pkinase. DR Pfam; PF00069; Pkinase; 1. DR PRINTS; PR00109; TYRKINASE. DR ProDom; PD000001; Prot_kinase; 1. DR SMART; SM00220; S_TKc; 1. DR SMART; SM00219; TyrKc; 1. DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1. DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1. DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1. KW Alternative splicing; ATP-binding; Coiled coil; Kinase; KW Serine/threonine-protein kinase; Transferase. FT DOMAIN 69 331 Protein kinase. FT NP_BIND 75 83 ATP (By similarity). FT DOMAIN 101 131 Coiled coil (Potential). FT DOMAIN 620 643 Coiled coil (Potential). FT ACT_SITE 197 197 Proton acceptor (By similarity). FT BINDING 98 98 ATP (By similarity). FT VARSPLIC 372 376 VGDMC -> MMRIS (in isoform 1S). FT /FTId=VSP_010124. FT VARSPLIC 377 666 Missing (in isoform 1S). FT /FTId=VSP_010125. SQ SEQUENCE 666 AA; 73469 MW; 588C6339CDC58C18 CRC64; MQDFFGSVRR SLVFRPSSDD DNQENQPPFP GVLADKITSC IRKSKIFIKP SFSPPPPANT VDMAPPISWR KGQLIGRGAF GTVYMGMNLD SGELLAVKQV LIAANFASKE KTQAHIQELE EEVKLLKNLS HPNIVRYLGT VREDDTLNIL LEFVPGGSIS SLLEKFGPFP ESVVRTYTRQ LLLGLEYLHN HAIMHRDIKG ANILVDNKGC IKLADFGASK QVAELATMTG AKSMKGTPYW MAPEVILQTG HSFSADIWSV GCTVIEMVTG KAPWSQQYKE VAAIFFIGTT KSHPPIPDTL SSDAKDFLLK CLQEVPNLRP TASELLKHPF VMGKHKESAS TDLGSVLNNL STPLPLQINN TKSTPDSTCD DVGDMCNFGS LNYSLVDPVK SIQNKNLWQQ NDNGGDEDDM CLIDDENFLT FDGEMSSTLE KDCHLKKSCD DISDMSIALK SKFDESPGNG EKESTMSMEC DQPSYSEDDD ELTESKIKAF LDEKAADLKK LQTPLYEEFY NSLITFSPSC MESNLSNSKR EDTARGFLKL PPKSRSPSRG PLGGSPSRAT DATSCSKSPG SGGSRELNIN NGGDEASQDG VSARVTDWRG LVVDTKQELS QCVALSEIEK KWKEELDQEL ERKRQEIMRQ AGLGSSPRDR GMSRQREKSR FASPGK //