ID DAD1_CHICK Reviewed; 123 AA. AC O13113; DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot. DT 01-JUL-1997, sequence version 1. DT 22-JUL-2015, entry version 85. DE RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; DE Short=Oligosaccharyl transferase subunit DAD1; DE EC=2.4.99.18; DE AltName: Full=Defender against cell death 1; DE Short=DAD-1; GN Name=DAD1; OS Gallus gallus (Chicken). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; OC Phasianidae; Phasianinae; Gallus. OX NCBI_TaxID=9031; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=9271627; DOI=10.1007/s002510050291; RA Wang K., Gan L., Kuo C.L., Hood L.; RT "A highly conserved apoptotic suppressor gene is located near the RT chicken T-cell receptor alpha chain constant region."; RL Immunogenetics 46:376-382(1997). CC -!- FUNCTION: Essential subunit of the N-oligosaccharyl transferase CC (OST) complex which catalyzes the transfer of a high mannose CC oligosaccharide from a lipid-linked oligosaccharide donor to an CC asparagine residue within an Asn-X-Ser/Thr consensus motif in CC nascent polypeptide chains. N-glycosylation occurs CC cotranslationally and the complex associates with the Sec61 CC complex at the channel-forming translocon complex that mediates CC protein translocation across the endoplasmic reticulum (ER). Loss CC of the DAD1 protein triggers apoptosis (By similarity). CC {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: Dolichyl diphosphooligosaccharide + [protein]- CC L-asparagine = dolichyl diphosphate + a glycoprotein with the CC oligosaccharide chain attached by N-beta-D-glycosyl linkage to a CC protein L-asparagine. CC -!- PATHWAY: Protein modification; protein glycosylation. CC -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex. CC OST seems to exist in different forms which contain at least RPN1, CC RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST CC can form stable complexes with the Sec61 complex or with both the CC Sec61 and TRAP complexes (By similarity). {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. CC -!- SIMILARITY: Belongs to the DAD/OST2 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U83627; AAC60276.1; -; mRNA. DR RefSeq; NP_001007474.1; NM_001007473.1. DR UniGene; Gga.4960; -. DR STRING; 9031.ENSGALP00000040147; -. DR PaxDb; O13113; -. DR PRIDE; O13113; -. DR Ensembl; ENSGALT00000000262; ENSGALP00000000261; ENSGALG00000000196. DR GeneID; 395343; -. DR KEGG; gga:395343; -. DR CTD; 1603; -. DR eggNOG; NOG246737; -. DR GeneTree; ENSGT00390000003324; -. DR HOGENOM; HOG000211854; -. DR HOVERGEN; HBG004452; -. DR InParanoid; O13113; -. DR KO; K12668; -. DR OMA; HIVVINF; -. DR OrthoDB; EOG7WT43N; -. DR PhylomeDB; O13113; -. DR UniPathway; UPA00378; -. DR NextBio; 20815428; -. DR PRO; PR:O13113; -. DR Proteomes; UP000000539; Chromosome 27. DR ExpressionAtlas; O13113; baseline. DR GO; GO:0070062; C:extracellular exosome; IEA:Ensembl. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0008250; C:oligosaccharyltransferase complex; IEA:InterPro. DR GO; GO:0004579; F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; IEA:InterPro. DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW. DR GO; GO:0043066; P:negative regulation of apoptotic process; IEA:Ensembl. DR GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway. DR InterPro; IPR003038; DAD/Ost2. DR PANTHER; PTHR10705; PTHR10705; 1. DR Pfam; PF02109; DAD; 1. DR PIRSF; PIRSF005588; DAD; 1. PE 2: Evidence at transcript level; KW Apoptosis; Complete proteome; Endoplasmic reticulum; KW Glycosyltransferase; Membrane; Reference proteome; Transferase; KW Transmembrane; Transmembrane helix. FT CHAIN 1 123 Dolichyl-diphosphooligosaccharide-- FT protein glycosyltransferase subunit DAD1. FT /FTId=PRO_0000124015. FT TOPO_DOM 1 40 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 41 61 Helical. {ECO:0000255}. FT TOPO_DOM 62 62 Lumenal. {ECO:0000255}. FT TRANSMEM 63 83 Helical. {ECO:0000255}. FT TOPO_DOM 84 102 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 103 123 Helical. {ECO:0000255}. SQ SEQUENCE 123 AA; 12926 MW; D468651E6A5D01C5 CRC64; MSGTAGSGVG AAGSVGSVVR RFLAEYGSGT SSRLKVLDAY LLYVMLTGAL QFGYCLGVGT FPFNSFLSGF ISAVGSFILG VCLRIQINPQ NKGEFQGISP ERAFADFLFA NTILHLVVIN FVG //