ID DAD1_CHICK Reviewed; 123 AA. AC O13113; DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot. DT 01-JUL-1997, sequence version 1. DT 01-SEP-2009, entry version 47. DE RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; DE Short=Oligosaccharyl transferase subunit DAD1; DE EC=2.4.1.119; DE AltName: Full=Defender against cell death 1; DE Short=DAD-1; GN Name=DAD1; OS Gallus gallus (Chicken). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archosauria; Dinosauria; Saurischia; Theropoda; Coelurosauria; Aves; OC Neognathae; Galliformes; Phasianidae; Phasianinae; Gallus. OX NCBI_TaxID=9031; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX MEDLINE=97419167; PubMed=9271627; DOI=10.1007/s002510050291; RA Wang K., Gan L., Kuo C.L., Hood L.; RT "A highly conserved apoptotic suppressor gene is located near the RT chicken T-cell receptor alpha chain constant region."; RL Immunogenetics 46:376-382(1997). CC -!- FUNCTION: Component of the N-oligosaccharyl transferase enzyme CC which catalyzes the transfer of a high mannose oligosaccharide CC from a lipid-linked oligosaccharide donor to an asparagine residue CC within an Asn-X-Ser/Thr consensus motif in nascent polypeptide CC chains. N-glycosylation occurs cotranslationally and the complex CC associates with the Sec61 complex at the channel-forming CC translocon complex that mediates protein translocation across the CC endoplasmic reticulum (ER). Loss of the DAD1 protein triggers CC apoptosis (By similarity). CC -!- CATALYTIC ACTIVITY: Dolichyl diphosphooligosaccharide + protein L- CC asparagine = dolichyl diphosphate + a glycoprotein with the CC oligosaccharide chain attached by N-glycosyl linkage to protein L- CC asparagine. CC -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex. CC OST seems to exist in different forms which contain at least RPN1, CC RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST CC can form stable complexes with the Sec61 complex or with both the CC Sec61 and TRAP complexes (By similarity). CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein CC (Potential). CC -!- SIMILARITY: Belongs to the DAD/OST2 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U83627; AAC60276.1; -; mRNA. DR IPI; IPI00575128; -. DR RefSeq; NP_001007474.1; -. DR UniGene; Gga.4960; -. DR STRING; O13113; -. DR Ensembl; ENSGALT00000000262; ENSGALP00000000261; ENSGALG00000000196; Gallus gallus. DR Ensembl; ENSGALT00000040942; ENSGALP00000040147; ENSGALG00000000196; Gallus gallus. DR GeneID; 395343; -. DR KEGG; gga:395343; -. DR CTD; 395343; -. DR HOGENOM; O13113; -. DR HOVERGEN; O13113; -. DR BRENDA; 2.4.1.119; 4. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. DR GO; GO:0004579; F:dolichyl-diphosphooligosaccharide-protein g...; IEA:EC. DR GO; GO:0006915; P:apoptosis; IEA:UniProtKB-KW. DR InterPro; IPR003038; DAD. DR PANTHER; PTHR10705; DAD; 1. DR Pfam; PF02109; DAD; 1. DR PIRSF; PIRSF005588; DAD; 1. PE 2: Evidence at transcript level; KW Apoptosis; Membrane; Transferase; Transmembrane. FT CHAIN 1 123 Dolichyl-diphosphooligosaccharide-- FT protein glycosyltransferase subunit DAD1. FT /FTId=PRO_0000124015. FT TRANSMEM 41 61 Potential. FT TRANSMEM 63 83 Potential. SQ SEQUENCE 123 AA; 12926 MW; D468651E6A5D01C5 CRC64; MSGTAGSGVG AAGSVGSVVR RFLAEYGSGT SSRLKVLDAY LLYVMLTGAL QFGYCLGVGT FPFNSFLSGF ISAVGSFILG VCLRIQINPQ NKGEFQGISP ERAFADFLFA NTILHLVVIN FVG //