ID DAD1_CHICK Reviewed; 123 AA. AC O13113; DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot. DT 01-JUL-1997, sequence version 1. DT 10-FEB-2009, entry version 44. DE RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; DE Short=Oligosaccharyl transferase subunit DAD1; DE EC=2.4.1.119; DE AltName: Full=Defender against cell death 1; DE Short=DAD-1; GN Name=DAD1; OS Gallus gallus (Chicken). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archosauria; Dinosauria; Saurischia; Theropoda; Coelurosauria; Aves; OC Neognathae; Galliformes; Phasianidae; Phasianinae; Gallus. OX NCBI_TaxID=9031; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX MEDLINE=97419167; PubMed=9271627; DOI=10.1007/s002510050291; RA Wang K., Gan L., Kuo C.L., Hood L.; RT "A highly conserved apoptotic suppressor gene is located near the RT chicken T-cell receptor alpha chain constant region."; RL Immunogenetics 46:376-382(1997). CC -!- FUNCTION: Component of the N-oligosaccharyl transferase enzyme CC which catalyzes the transfer of a high mannose oligosaccharide CC from a lipid-linked oligosaccharide donor to an asparagine residue CC within an Asn-X-Ser/Thr consensus motif in nascent polypeptide CC chains. N-glycosylation occurs cotranslationally and the complex CC associates with the Sec61 complex at the channel-forming CC translocon complex that mediates protein translocation across the CC endoplasmic reticulum (ER). Loss of the DAD1 protein triggers CC apoptosis (By similarity). CC -!- CATALYTIC ACTIVITY: Dolichyl diphosphooligosaccharide + protein L- CC asparagine = dolichyl diphosphate + a glycoprotein with the CC oligosaccharide chain attached by N-glycosyl linkage to protein L- CC asparagine. CC -!- SUBUNIT: Component of the oligosaccharyl transferase (OST) complex CC (By similarity). OST seems to exist in different forms which CC contain at least RPN1/ribophorin I, RPN2/ribophorin II, OST48, CC DAD1, and either STT3A or STT3B. CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein CC (Potential). CC -!- SIMILARITY: Belongs to the DAD/OST2 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U83627; AAC60276.1; -; mRNA. DR IPI; IPI00575128; -. DR RefSeq; NP_001007474.1; -. DR UniGene; Gga.4960; -. DR Ensembl; ENSGALG00000000196; Gallus gallus. DR GeneID; 395343; -. DR KEGG; gga:395343; -. DR HOGENOM; O13113; -. DR HOVERGEN; O13113; -. DR BRENDA; 2.4.1.119; 4. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. DR GO; GO:0004579; F:dolichyl-diphosphooligosaccharide-protein g...; IEA:EC. DR GO; GO:0006915; P:apoptosis; IEA:UniProtKB-KW. DR InterPro; IPR003038; DAD. DR PANTHER; PTHR10705; DAD; 1. DR Pfam; PF02109; DAD; 1. DR PIRSF; PIRSF005588; DAD; 1. PE 2: Evidence at transcript level; KW Apoptosis; Membrane; Transferase; Transmembrane. FT CHAIN 1 123 Dolichyl-diphosphooligosaccharide-- FT protein glycosyltransferase subunit DAD1. FT /FTId=PRO_0000124015. FT TRANSMEM 41 61 Potential. FT TRANSMEM 63 83 Potential. SQ SEQUENCE 123 AA; 12926 MW; D468651E6A5D01C5 CRC64; MSGTAGSGVG AAGSVGSVVR RFLAEYGSGT SSRLKVLDAY LLYVMLTGAL QFGYCLGVGT FPFNSFLSGF ISAVGSFILG VCLRIQINPQ NKGEFQGISP ERAFADFLFA NTILHLVVIN FVG //