ID DAD1_CHICK Reviewed; 123 AA. AC O13113; DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot. DT 01-JUL-1997, sequence version 1. DT 20-JUN-2018, entry version 101. DE RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; DE Short=Oligosaccharyl transferase subunit DAD1; DE AltName: Full=Defender against cell death 1; DE Short=DAD-1; GN Name=DAD1; OS Gallus gallus (Chicken). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; OC Phasianidae; Phasianinae; Gallus. OX NCBI_TaxID=9031; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=9271627; DOI=10.1007/s002510050291; RA Wang K., Gan L., Kuo C.L., Hood L.; RT "A highly conserved apoptotic suppressor gene is located near the RT chicken T-cell receptor alpha chain constant region."; RL Immunogenetics 46:376-382(1997). CC -!- FUNCTION: Essential subunit of the N-oligosaccharyl transferase CC (OST) complex which catalyzes the transfer of a high mannose CC oligosaccharide from a lipid-linked oligosaccharide donor to an CC asparagine residue within an Asn-X-Ser/Thr consensus motif in CC nascent polypeptide chains. Required for the assembly of both CC SST3A- and SS3B-containing OST complexes. Required for efficient CC N-glycosylation. Loss of the DAD1 protein triggers apoptosis. CC {ECO:0000250|UniProtKB:P61803}. CC -!- PATHWAY: Protein modification; protein glycosylation. CC -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex. CC OST exists in two different complex forms which contain common CC core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either CC STT3A or STT3B as catalytic subunits, and form-specific accessory CC subunits. OST can form stable complexes with the Sec61 complex or CC with both the Sec61 and TRAP complexes (By similarity). CC {ECO:0000250|UniProtKB:P61803}. CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass CC membrane protein {ECO:0000305}. CC -!- SIMILARITY: Belongs to the DAD/OST2 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U83627; AAC60276.1; -; mRNA. DR RefSeq; NP_001007474.1; NM_001007473.1. DR UniGene; Gga.4960; -. DR SMR; O13113; -. DR STRING; 9031.ENSGALP00000040147; -. DR PaxDb; O13113; -. DR PRIDE; O13113; -. DR Ensembl; ENSGALT00000048785; ENSGALP00000052681; ENSGALG00000035626. DR GeneID; 395343; -. DR KEGG; gga:395343; -. DR CTD; 1603; -. DR eggNOG; KOG1746; Eukaryota. DR eggNOG; ENOG4111Q2I; LUCA. DR GeneTree; ENSGT00390000003324; -. DR HOGENOM; HOG000211854; -. DR HOVERGEN; HBG004452; -. DR InParanoid; O13113; -. DR KO; K12668; -. DR OMA; GVCLRLQ; -. DR OrthoDB; EOG091G197A; -. DR PhylomeDB; O13113; -. DR UniPathway; UPA00378; -. DR PRO; PR:O13113; -. DR Proteomes; UP000000539; Chromosome 27. DR Bgee; ENSGALG00000000196; -. DR ExpressionAtlas; O13113; baseline and differential. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0008250; C:oligosaccharyltransferase complex; IBA:GO_Central. DR GO; GO:0004579; F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; IEA:InterPro. DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW. DR GO; GO:0043066; P:negative regulation of apoptotic process; IEA:Ensembl. DR GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central. DR InterPro; IPR003038; DAD/Ost2. DR PANTHER; PTHR10705; PTHR10705; 1. DR Pfam; PF02109; DAD; 1. DR PIRSF; PIRSF005588; DAD; 1. PE 2: Evidence at transcript level; KW Apoptosis; Complete proteome; Endoplasmic reticulum; Membrane; KW Reference proteome; Transmembrane; Transmembrane helix. FT CHAIN 1 123 Dolichyl-diphosphooligosaccharide-- FT protein glycosyltransferase subunit DAD1. FT /FTId=PRO_0000124015. FT TOPO_DOM 1 40 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 41 61 Helical. {ECO:0000255}. FT TOPO_DOM 62 62 Lumenal. {ECO:0000255}. FT TRANSMEM 63 83 Helical. {ECO:0000255}. FT TOPO_DOM 84 102 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 103 123 Helical. {ECO:0000255}. SQ SEQUENCE 123 AA; 12926 MW; D468651E6A5D01C5 CRC64; MSGTAGSGVG AAGSVGSVVR RFLAEYGSGT SSRLKVLDAY LLYVMLTGAL QFGYCLGVGT FPFNSFLSGF ISAVGSFILG VCLRIQINPQ NKGEFQGISP ERAFADFLFA NTILHLVVIN FVG //