ID N9U355_9GAMM Unreviewed; 808 AA. AC N9U355; DT 26-JUN-2013, integrated into UniProtKB/TrEMBL. DT 26-JUN-2013, sequence version 1. DT 31-JAN-2018, entry version 30. DE RecName: Full=Bifunctional aspartokinase/homoserine dehydrogenase {ECO:0000256|PIRNR:PIRNR000727}; DE Includes: DE RecName: Full=Aspartokinase {ECO:0000256|PIRNR:PIRNR000727}; DE EC=2.7.2.4 {ECO:0000256|PIRNR:PIRNR000727}; DE Includes: DE RecName: Full=Homoserine dehydrogenase {ECO:0000256|PIRNR:PIRNR000727}; DE EC=1.1.1.3 {ECO:0000256|PIRNR:PIRNR000727}; GN Name=metL {ECO:0000313|EMBL:ENY72725.1}; GN ORFNames=G114_06497 {ECO:0000313|EMBL:ENY72725.1}; OS Aeromonas diversa CDC 2478-85. OC Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales; OC Aeromonadaceae; Aeromonas. OX NCBI_TaxID=1268237 {ECO:0000313|EMBL:ENY72725.1, ECO:0000313|Proteomes:UP000023775}; RN [1] {ECO:0000313|EMBL:ENY72725.1, ECO:0000313|Proteomes:UP000023775} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=2478-85 {ECO:0000313|EMBL:ENY72725.1, RC ECO:0000313|Proteomes:UP000023775}; RX PubMed=23792745; DOI=10.1128/genomeA.00330-13; RA Farfan M., Spataro N., Sanglas A., Albarral V., Loren J.G., Bosch E., RA Fuste M.C.; RT "Draft Genome Sequence of the Aeromonas diversa Type Strain."; RL Genome Announc. 1:S69-S82(2013). CC -!- CATALYTIC ACTIVITY: ATP + L-aspartate = ADP + 4-phospho-L- CC aspartate. {ECO:0000256|PIRNR:PIRNR000727}. CC -!- CATALYTIC ACTIVITY: L-homoserine + NAD(P)(+) = L-aspartate 4- CC semialdehyde + NAD(P)H. {ECO:0000256|PIRNR:PIRNR000727}. CC -!- PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP CC pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4. CC {ECO:0000256|PIRNR:PIRNR000727}. CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de CC novo pathway; L-homoserine from L-aspartate: step 1/3. CC {ECO:0000256|PIRNR:PIRNR000727}. CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de CC novo pathway; L-homoserine from L-aspartate: step 3/3. CC {ECO:0000256|PIRNR:PIRNR000727}. CC -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L- CC threonine from L-aspartate: step 1/5. CC {ECO:0000256|PIRNR:PIRNR000727}. CC -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L- CC threonine from L-aspartate: step 3/5. CC {ECO:0000256|PIRNR:PIRNR000727}. CC -!- SUBUNIT: Homotetramer. {ECO:0000256|PIRNR:PIRNR000727}. CC -!- SIMILARITY: In the C-terminal section; belongs to the homoserine CC dehydrogenase family. {ECO:0000256|PIRNR:PIRNR000727}. CC -!- SIMILARITY: In the N-terminal section; belongs to the CC aspartokinase family. {ECO:0000256|PIRNR:PIRNR000727}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:ENY72725.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; APVG01000012; ENY72725.1; -; Genomic_DNA. DR EnsemblBacteria; ENY72725; ENY72725; G114_06497. DR PATRIC; fig|1268237.3.peg.1278; -. DR OrthoDB; POG091H0223; -. DR UniPathway; UPA00034; UER00015. DR UniPathway; UPA00050; UER00063. DR UniPathway; UPA00051; UER00462. DR Proteomes; UP000023775; Unassembled WGS sequence. DR GO; GO:0004072; F:aspartate kinase activity; IEA:InterPro. DR GO; GO:0004412; F:homoserine dehydrogenase activity; IEA:UniProtKB-EC. DR GO; GO:0050661; F:NADP binding; IEA:InterPro. DR GO; GO:0009067; P:aspartate family amino acid biosynthetic process; IEA:InterPro. DR Gene3D; 3.40.1160.10; -; 1. DR InterPro; IPR036393; AceGlu_kinase-like_sf. DR InterPro; IPR001048; Asp/Glu/Uridylate_kinase. DR InterPro; IPR005106; Asp/hSer_DH_NAD-bd. DR InterPro; IPR001341; Asp_kinase. DR InterPro; IPR018042; Aspartate_kinase_CS. DR InterPro; IPR011147; Bifunc_aspartokin/hSer_DH. DR InterPro; IPR001342; HDH_cat. DR InterPro; IPR019811; HDH_CS. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR Pfam; PF00696; AA_kinase; 1. DR Pfam; PF00742; Homoserine_dh; 1. DR Pfam; PF03447; NAD_binding_3; 1. DR PIRSF; PIRSF000727; ThrA; 1. DR SUPFAM; SSF51735; SSF51735; 1. DR SUPFAM; SSF53633; SSF53633; 1. DR TIGRFAMs; TIGR00657; asp_kinases; 1. DR PROSITE; PS00324; ASPARTOKINASE; 1. DR PROSITE; PS01042; HOMOSER_DHGENASE; 1. PE 3: Inferred from homology; KW Amino-acid biosynthesis {ECO:0000256|PIRNR:PIRNR000727}; KW ATP-binding {ECO:0000256|PIRNR:PIRNR000727}; KW Coiled coil {ECO:0000256|SAM:Coils}; KW Complete proteome {ECO:0000313|Proteomes:UP000023775}; KW Kinase {ECO:0000256|PIRNR:PIRNR000727, ECO:0000313|EMBL:ENY72725.1}; KW NADP {ECO:0000256|PIRNR:PIRNR000727}; KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR000727}; KW Oxidoreductase {ECO:0000256|PIRNR:PIRNR000727, KW ECO:0000313|EMBL:ENY72725.1}; KW Reference proteome {ECO:0000313|Proteomes:UP000023775}; KW Transferase {ECO:0000256|PIRNR:PIRNR000727}. FT DOMAIN 10 279 AA_kinase. {ECO:0000259|Pfam:PF00696}. FT DOMAIN 465 597 NAD_binding_3. {ECO:0000259|Pfam: FT PF03447}. FT DOMAIN 606 801 Homoserine_dh. {ECO:0000259|Pfam: FT PF00742}. FT COILED 101 121 {ECO:0000256|SAM:Coils}. FT COILED 474 494 {ECO:0000256|SAM:Coils}. SQ SEQUENCE 808 AA; 89239 MW; EDA418A3F2545555 CRC64; MAAEVSVKRR HVHKFGGSSL ADPVCYRRVA AIVEQQSGGN ELVVVSAAGK TTNRLIQLVE LAEGGDEAAG EAHTALHAYQ QSLIDGLLEG DLQADLRAQL AEDFRTIART LERQFDRFER NGLLAFGEVW SARLLAALLT QRGNPTLWLD ARSFLRAEDG PLIRVDHALS SELLKARLAE HDGRIVVTGF IAADLEGRSL LLGRNGSDYS ASLLAALADG ESTTIWSDVA GVYSADPRRV KEARLLERLS LAEANELARL GSSVLHSRTL QPVVDSRQRL TLRCSYNPDE GCTHILRRAP RSGGARIVSS VDQIALIELK VLPEGEFERA AAAIEAYLAR HRIQPLTAQR QPDRRMLRLA YTLEVASGAY DLLRDYQLEG GFTGLILKEG YSLVALVGAG VTENAEQCHR FYQLTADQPV EFVQAAKDSL SLVAVLRQIV LEPLLIALHG ALFSRPTRVG LVVFGKGNIG GHWLSLYNRE RQRLEQELNL ALTLYGVYGS EGGWLDEEGL DPLRVQEASN PAPLIWPELL GQLEQHSFDA LIALDLTASE TVSRYYPDLA QLGIHLIAAN KFAGAAESEF YQRVKQSCRD HQVQWRYNAT VGAGLPVQSS IQMLRQSGDR IHGVSGIFSG TLSWLFQQYD GCRPFSELVE EAWQHGLTEP DPREDLSGQD VRRKLLILAR ESGYEIDAND IELENLVPVP LRKLGPDQFM DRLKELDGPI LSAFESARRL GKVLRYVARF EHDGKARVGL ESLEPHHPFA NLLPCDNIFA IESDSYRTNP LVIQGPGAGR EVTAAAIQYD LWQICASL //