ID N6ULG1_9RHIZ Unreviewed; 299 AA. AC N6ULG1; DT 26-JUN-2013, integrated into UniProtKB/TrEMBL. DT 26-JUN-2013, sequence version 1. DT 18-JAN-2017, entry version 27. DE RecName: Full=Bifunctional protein FolD {ECO:0000256|HAMAP-Rule:MF_01576}; DE Includes: DE RecName: Full=Methenyltetrahydrofolate cyclohydrolase {ECO:0000256|HAMAP-Rule:MF_01576}; DE EC=3.5.4.9 {ECO:0000256|HAMAP-Rule:MF_01576}; DE Includes: DE RecName: Full=Methylenetetrahydrofolate dehydrogenase {ECO:0000256|HAMAP-Rule:MF_01576}; DE EC=1.5.1.5 {ECO:0000256|HAMAP-Rule:MF_01576}; GN Name=folD {ECO:0000256|HAMAP-Rule:MF_01576, GN ECO:0000313|EMBL:ENN93249.1}; GN ORFNames=m02_02550 {ECO:0000313|EMBL:ENN93249.1}; OS Bartonella bovis m02. OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Bartonellaceae; Bartonella. OX NCBI_TaxID=1094492 {ECO:0000313|EMBL:ENN93249.1, ECO:0000313|Proteomes:UP000014026}; RN [1] {ECO:0000313|EMBL:ENN93249.1, ECO:0000313|Proteomes:UP000014026} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=m02 {ECO:0000313|Proteomes:UP000014026}; RX PubMed=23555299; DOI=10.1371/journal.pgen.1003393; RA Guy L., Nystedt B., Toft C., Zaremba-Niedzwiedzka K., Berglund E.C., RA Granberg F., Naslund K., Eriksson A.S., Andersson S.G.; RT "A gene transfer agent and a dynamic repertoire of secretion systems RT hold the keys to the explosive radiation of the emerging pathogen RT Bartonella."; RL PLoS Genet. 9:E1003393-E1003393(2013). CC -!- FUNCTION: Catalyzes the oxidation of 5,10- CC methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and CC then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- CC formyltetrahydrofolate. {ECO:0000256|HAMAP-Rule:MF_01576, CC ECO:0000256|SAAS:SAAS00217579}. CC -!- CATALYTIC ACTIVITY: 5,10-methenyltetrahydrofolate + H(2)O = 10- CC formyltetrahydrofolate. {ECO:0000256|HAMAP-Rule:MF_01576, CC ECO:0000256|SAAS:SAAS00020423}. CC -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + NADP(+) = CC 5,10-methenyltetrahydrofolate + NADPH. {ECO:0000256|HAMAP- CC Rule:MF_01576, ECO:0000256|SAAS:SAAS00217562}. CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion. CC {ECO:0000256|HAMAP-Rule:MF_01576, ECO:0000256|SAAS:SAAS00217553}. CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01576, CC ECO:0000256|SAAS:SAAS00217560}. CC -!- SIMILARITY: Belongs to the tetrahydrofolate CC dehydrogenase/cyclohydrolase family. {ECO:0000256|HAMAP- CC Rule:MF_01576, ECO:0000256|SAAS:SAAS00635342}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation CC of feature annotation. {ECO:0000256|HAMAP-Rule:MF_01576}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:ENN93249.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AGWB01000003; ENN93249.1; -; Genomic_DNA. DR RefSeq; WP_010702073.1; NZ_KB915625.1. DR EnsemblBacteria; ENN93249; ENN93249; m02_02550. DR OrthoDB; POG091H0041; -. DR UniPathway; UPA00193; -. DR Proteomes; UP000014026; Unassembled WGS sequence. DR GO; GO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:UniProtKB-HAMAP. DR GO; GO:0009396; P:folic acid-containing compound biosynthetic process; IEA:InterPro. DR GO; GO:0000105; P:histidine biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0006164; P:purine nucleotide biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. DR Gene3D; 3.40.50.720; -; 1. DR HAMAP; MF_01576; THF_DHG_CYH; 1. DR InterPro; IPR016040; NAD(P)-bd_dom. DR InterPro; IPR000672; THF_DH/CycHdrlase. DR InterPro; IPR020630; THF_DH/CycHdrlase_cat_dom. DR InterPro; IPR020867; THF_DH/CycHdrlase_CS. DR InterPro; IPR020631; THF_DH/CycHdrlase_NAD-bd_dom. DR Pfam; PF00763; THF_DHG_CYH; 1. DR Pfam; PF02882; THF_DHG_CYH_C; 1. DR PRINTS; PR00085; THFDHDRGNASE. DR SUPFAM; SSF51735; SSF51735; 1. DR PROSITE; PS00766; THF_DHG_CYH_1; 1. DR PROSITE; PS00767; THF_DHG_CYH_2; 1. PE 3: Inferred from homology; KW Amino-acid biosynthesis {ECO:0000256|HAMAP-Rule:MF_01576, KW ECO:0000256|SAAS:SAAS00217554}; KW Complete proteome {ECO:0000313|Proteomes:UP000014026}; KW Histidine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01576, KW ECO:0000256|SAAS:SAAS00217571}; KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_01576, KW ECO:0000256|SAAS:SAAS00020439}; KW Methionine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01576, KW ECO:0000256|SAAS:SAAS00217556}; KW Multifunctional enzyme {ECO:0000256|HAMAP-Rule:MF_01576, KW ECO:0000256|SAAS:SAAS00020419}; KW NADP {ECO:0000256|HAMAP-Rule:MF_01576, ECO:0000256|SAAS:SAAS00217574}; KW One-carbon metabolism {ECO:0000256|HAMAP-Rule:MF_01576, KW ECO:0000256|SAAS:SAAS00020466}; KW Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_01576, KW ECO:0000256|SAAS:SAAS00020431}; KW Purine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01576, KW ECO:0000256|SAAS:SAAS00217555}. FT DOMAIN 4 120 THF_DHG_CYH. {ECO:0000259|Pfam:PF00763}. FT DOMAIN 123 290 THF_DHG_CYH_C. {ECO:0000259|Pfam: FT PF02882}. FT NP_BIND 168 170 NADP. {ECO:0000256|HAMAP-Rule:MF_01576}. FT BINDING 193 193 NADP. {ECO:0000256|HAMAP-Rule:MF_01576}. FT BINDING 234 234 NADP; via amide nitrogen. FT {ECO:0000256|HAMAP-Rule:MF_01576}. SQ SEQUENCE 299 AA; 32266 MW; 4476096F7DA20608 CRC64; MDNIIDGKKL AENTINKVKK ETKKFRNDHK IQPGIAVIIV GNDPASQVYV TSKSKKAEEC GFLSIKHTIP QDTKEEELLQ LIETLNSDPK IHGILVQLPL PAHINTNKIT QAIAVQKDVD GFHYINIGKL AANIFEDTII PCTPAGVMMI IEQQCGQDLS GLDAVIVGRS NIVGKPMAAL LMAANATVTI AHSRTRDLDE VCRSADILVV AVGCPHIIKK DWVKQGAIVI DVGINRIEAS EKGTNKTRLV GDVDFAEVKE KAAAITPVPG GVGPMTIAML MVNTLKAAAR AHKLPIPKF //