ID M9VZ69_CHIKV Unreviewed; 2470 AA. AC M9VZ69; DT 26-JUN-2013, integrated into UniProtKB/TrEMBL. DT 26-JUN-2013, sequence version 1. DT 03-JUL-2019, entry version 37. DE SubName: Full=Nonstructural polyprotein {ECO:0000313|EMBL:AGJ84081.1}; OS Chikungunya virus (CHIKV). OC Viruses; Riboviria; Togaviridae; Alphavirus. OX NCBI_TaxID=37124 {ECO:0000313|EMBL:AGJ84081.1, ECO:0000313|Proteomes:UP000098741}; OH NCBI_TaxID=7159; Aedes aegypti (Yellowfever mosquito) (Culex aegypti). OH NCBI_TaxID=7160; Aedes albopictus (Asian tiger mosquito) (Stegomyia albopicta). OH NCBI_TaxID=299627; Aedes furcifer (Mosquito). OH NCBI_TaxID=188700; Aedes polynesiensis (Polynesian tiger mosquito). OH NCBI_TaxID=9533; Cercopithecus. OH NCBI_TaxID=9606; Homo sapiens (Human). OH NCBI_TaxID=9539; Macaca (macaques). OH NCBI_TaxID=9598; Pan troglodytes (Chimpanzee). OH NCBI_TaxID=9554; Papio (baboons). OH NCBI_TaxID=9573; Presbytis. RN [1] {ECO:0000313|EMBL:AGJ84081.1, ECO:0000313|Proteomes:UP000098741} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CHIKV-JC2012 {ECO:0000313|EMBL:AGJ84081.1}; RA Zhou D., Sun D., Zheng J., Ni M., Qiu J., Wan Y., Yang T., Long Z., RA Zai M.; RT "Sequence analysis of Chikungunya virus strain CHIKV-JC2012."; RL Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; CC Evidence={ECO:0000256|SAAS:SAAS01199057}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:11128, Rhea:RHEA- CC COMP:11129, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, CC ChEBI:CHEBI:83400; EC=2.7.7.48; CC Evidence={ECO:0000256|SAAS:SAAS01198479}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KC488650; AGJ84081.1; -; Genomic_RNA. DR Proteomes; UP000098741; Genome. DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-KW. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW. DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:UniProtKB-UniRule. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0006396; P:RNA processing; IEA:InterPro. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom. DR InterPro; IPR002588; Alphavirus-like_MT_dom. DR InterPro; IPR002620; Alphavirus_nsp2pro. DR InterPro; IPR002589; Macro_dom. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR007094; RNA-dir_pol_PSvirus. DR InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol. DR Pfam; PF01661; Macro; 1. DR Pfam; PF01707; Peptidase_C9; 1. DR Pfam; PF00978; RdRP_2; 1. DR Pfam; PF01443; Viral_helicase1; 1. DR Pfam; PF01660; Vmethyltransf; 1. DR SMART; SM00506; A1pp; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR PROSITE; PS51743; ALPHAVIRUS_MT; 1. DR PROSITE; PS51154; MACRO; 1. DR PROSITE; PS51520; NSP2PRO; 1. DR PROSITE; PS51657; PSRV_HELICASE; 1. DR PROSITE; PS50507; RDRP_SSRNA_POS; 1. PE 4: Predicted; KW ATP-binding {ECO:0000256|SAAS:SAAS01198603}; KW Complete proteome {ECO:0000313|Proteomes:UP000098741}; KW Helicase {ECO:0000256|SAAS:SAAS01196733}; KW Host membrane {ECO:0000256|SAAS:SAAS01196722}; KW Hydrolase {ECO:0000256|SAAS:SAAS01198589}; KW Membrane {ECO:0000256|SAAS:SAAS01196710}; KW Metal-binding {ECO:0000256|SAAS:SAAS01196717}; KW Nucleotide-binding {ECO:0000256|SAAS:SAAS01198580}; KW Nucleotidyltransferase {ECO:0000256|SAAS:SAAS01198562}; KW Protease {ECO:0000256|PROSITE-ProRule:PRU00853, KW ECO:0000256|SAAS:SAAS01196706}; KW RNA-directed RNA polymerase {ECO:0000256|SAAS:SAAS01198530}; KW Thiol protease {ECO:0000256|SAAS:SAAS01196707}; KW Transferase {ECO:0000256|SAAS:SAAS01198537}; KW Viral RNA replication {ECO:0000256|SAAS:SAAS01198628}; KW Zinc {ECO:0000256|SAAS:SAAS01196702}. FT DOMAIN 28 259 Alphavirus-like MT. {ECO:0000259|PROSITE: FT PS51743}. FT DOMAIN 690 991 (+)RNA virus helicase C-terminal. FT {ECO:0000259|PROSITE:PS51657}. FT DOMAIN 1004 1327 Peptidase C9. {ECO:0000259|PROSITE: FT PS51520}. FT DOMAIN 1324 1493 Macro. {ECO:0000259|PROSITE:PS51154}. FT DOMAIN 2224 2339 RdRp catalytic. {ECO:0000259|PROSITE: FT PS50507}. FT REGION 485 505 Disordered. {ECO:0000256|MobiDB-lite: FT M9VZ69}. FT ACT_SITE 1013 1013 For cysteine protease nsP2 activity. FT {ECO:0000256|PROSITE-ProRule:PRU00853}. FT ACT_SITE 1083 1083 For cysteine protease nsP2 activity. FT {ECO:0000256|PROSITE-ProRule:PRU00853}. SQ SEQUENCE 2470 AA; 275428 MW; 8F90B3C1ECC25235 CRC64; MDSVYVDIDA DSAFLKALQR AYPMFEVEPR QVTSNDHANA RAFSHLAIKL IEQEIDPDST ILDIGSAPAR RMMSDRKYHC VCPMRSAEDP ERLANYARKL ASAAGKVLDR NISGKIGDLQ EVMAVPDTET PTFCLHTDVS CRQRADVAIY QDVYAVHAPT SLYHQAIKGV RVAYWVGFDT TPFMYNAMAG AYPSYSTNWA DEQVLKAKNI GLCSTDLTEG RRGKLSIMRG KKLKPCDRVL FSVGSTLYPE SRMLLKSWHL PSVFHLKGKL SFTCRCDTVV SCEGYVVKRI TMSPGLYGKT TGYAVTHHAD GFLMCKTTDT VDGERVSFPV CTYVPATICD QMTGILATEV TPEDAQKLLV GLNQRIVVNG RTQRNTNTMK NYLLPVVAQA FSKWAKECRK DMEDEKLLGV RERTLTCCCL WAFKKQKTHT VYKRPDTQSI QKVQAEFDSF VVPGLWSSGL SIPLRTRIKW LLRKVPKADL IPYSGNAQEA QDAEKEAEEE REAELTHEAL PPLQAAQEDV QVEIDVEQLE DRAGAGIIET PRGAIKVTAQ LTDHVVGEYL VLSPQTVLRS QKLSLIHALA EQVKTCTHSG RVGRYAVEAY DGRVLVPSGY AISPEDFQSL SESATMVYNE REFVNRKLHH IAMHGPALNT DEESYELVRA ERTEHEYVYD VDQRRCCKKE EAAGLVLVGD LTNPPYHEFA YEGLKIRPAC PYKIAVIGVF GVPGSGKSAI IKNLVTRQDL VTSGKKENCQ EISTDVMRQR GLEISARTVD SLLLNGCNRP VDVLYVDEAF ACHSGTLLAL IALVRPRLKV VLCGDPKQCG FFNMMQMKVN YNHNICTQVY HKSISRRCTL PVTAIVSSLH YEGKMRTTNE YNMPIVVDTT GSTKPDPGDL VLTCFRGWVK QLQIDYRGHE VMTAAASQGL TRKGVYAVRQ KVNENPLYAS TSEHVNVLLT RTEGKLVWKT LSGDPWIKTL QNPPKGNFKA TIKEWEVEHA SIMAGICSHQ VTFDTFQNKA NVCWAKSLVP ILETAGIKLN DRQWSQIIQA FKEDKAYSPE VALNEICTRM YGVDLDSGLF SKPLVSVYYA DNHWDNRPGG KMFGFNPEAA SILERKYPFT KGKWNINKQI CVTTRRIEDF NPTTNIIPAN RRLPHSLVAE HRPVKGERME WLVNKINGHH VLLVSGYNLA LPTKRVTWVA PLGVRGADYT YNLEMGLPAT LGRYDLVVIN IHTPFRIHHY QQCVDHAMKL QMLGGDSLRL LKPGGSLLIR AYGYADRTSE RVICVLGRKF RSSRALKPPC ITSNTEMFFL FSSFDNGRRN FTTHVMNNQL NAAFVGQATR AGCAPSYRVK RMDIAKNDEE CVVNAANPRG LPGDGVCKAV YKKWPESFKN SATPVGTAKT VMCGTYPVIH AVGPNFSNYT ESEGDRELAA AYREVAKEVT RLGVNSVAIP LLSTGVYSGG KDRLTQSLNH LFTAMDSTDA DVVIYCRDKE WEKKISEAIQ MRTQVELLDE HISIDCDVIR VHPDSSLAGR KGYSTTEGAL YSYLEGTRFH QTAVDVAEIH TMWPKQIEAN EQVCLYALGE SIESIRQKCP VDDADASSPP KTVPCLCRYA MTPERVTRLR MNHVTNIIVC SSFPLPKYKI EGVQKVKCSK VMLFDHNVPS RVSPREYRSS QESVREVSMT TSLTHSQFDL SADGETLPVP SDLDADAPAL EPALDDGAIH TTGNLAAVSD WVMSTVPVAP PRRRRGRNLT VICDEREGNI TPMASVRFFR AEQCPTVQET AETRDTAISF RAPPSITVEL SHPPISFGAP SETFPITFGD FNDGEIESLS SELLTFGDFL PGEVDDLTDS DWSTCSDTDD ELXLDRAGGY IFSSDTGPGH LQQKSVRQSV LPVNTLEEVH EEKCYPPKLD ELKEQLLLKK LQESASTANR SRYQSRKVEN MKATIIQRLK RGCKLYLMAE TPKVPTYRTV YPAPVYSPPI NVRLSNPESA VAACNEFLAR NYPTVSSYQI TDEYDAYLDM VDGSESCLDR ATFNPSKLRS YPKQHAYHAP SIRSAVPSPF QNTLQNVLAA ATKRNCNVTQ MRELPTLDSA VFNVECFKKF ACNREYWEEF AASPIRITTE NLTTYVTKLK GPKAAALLAR THNLLPLQDV PMDRFTVDMK RDVKVTPGTK HTEERPKVQV IQAAEPLATA YLCGIHRELV RRLNAVLLPN VHTLFDMSAE DFDAIIAAHF KPGDAVLETD IASFDKSQDD SLALTALMLL EDLGVDHSLL DLIEAAFGEI SSCHLPTGTR FKFGAMMKSG MFLTLFVNTL LNITIASRVL EDRLTKSACA AFIGDDNIIH GVVSDELMAA RCATWMNMEV KIIDAVVSQK APYFCGGFIL HDTVTGTACR VADPLKRLFK LGKPLAAGDE QDEDRRRALA DEVIRWQRTG LIDELEKAVY SRYEVQGISV AVMSMATFAS SRSNFEKLRG PVITLYGGPK //