ID M9TGV3_MYCTX Unreviewed; 269 AA. AC M9TGV3; DT 26-JUN-2013, integrated into UniProtKB/TrEMBL. DT 26-JUN-2013, sequence version 1. DT 02-OCT-2024, entry version 91. DE RecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH] {ECO:0000256|ARBA:ARBA00017755, ECO:0000256|PIRNR:PIRNR000094}; DE EC=1.3.1.9 {ECO:0000256|ARBA:ARBA00012996, ECO:0000256|PIRNR:PIRNR000094}; GN Name=inhA {ECO:0000313|EMBL:AGJ50654.1}; GN Synonyms=inhA_2 {ECO:0000313|EMBL:OMH59404.1}; GN ORFNames=A4S10_01572 {ECO:0000313|EMBL:OMH59404.1}, DKC2_1580 GN {ECO:0000313|EMBL:VCU49751.1}, DSI28_03315 GN {ECO:0000313|EMBL:REM12503.1}, DSL01_08600 GN {ECO:0000313|EMBL:REX48956.1}, ERS007679_02961 GN {ECO:0000313|EMBL:COW03350.1}, ERS007681_02786 GN {ECO:0000313|EMBL:CFE41166.1}, ERS007688_00531 GN {ECO:0000313|EMBL:CFE46976.1}, ERS007718_00529 GN {ECO:0000313|EMBL:COV75687.1}, ERS007741_01725 GN {ECO:0000313|EMBL:COW17773.1}, ERS027659_00857 GN {ECO:0000313|EMBL:CKR17592.1}, ERS027661_03047 GN {ECO:0000313|EMBL:CKS41591.1}, ERS053720_00426 GN {ECO:0000313|EMBL:CFH70051.1}; OS Mycobacterium tuberculosis. OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae; OC Mycobacterium; Mycobacterium tuberculosis complex. OX NCBI_TaxID=1773 {ECO:0000313|EMBL:AGJ50654.1}; RN [1] {ECO:0000313|EMBL:AGJ50654.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=TB11-111 {ECO:0000313|EMBL:AGJ50654.1}, and TB11-153 RC {ECO:0000313|EMBL:AGJ50655.1}; RA Daum L.T., Rodriguez J.D., Bhattacharyya S.; RT "Ion Torrent full-gene sequencing and phenotypic drug susceptibility RT testing (DST) confirm multi-drug resistance (MDR) Mycobacterium RT tuberculosis in the United States."; RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:AHA90961.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=MtbPL.K131 {ECO:0000313|EMBL:AHA90961.1}; RX PubMed=24855126; DOI=10.1093/jac/dku161; RA Jagielski T., Bakula Z., Roeske K., Kaminski M., Napiorkowska A., RA Augustynowicz-Kopec E., Zwolska Z., Bielecki J.; RT "Detection of mutations associated with isoniazid resistance in multidrug- RT resistant Mycobacterium tuberculosis clinical isolates."; RL J. Antimicrob. Chemother. 69:2369-2375(2014). RN [3] {ECO:0000313|Proteomes:UP000045842, ECO:0000313|Proteomes:UP000046947} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Bir 185 {ECO:0000313|EMBL:CKR17592.1, RC ECO:0000313|Proteomes:UP000050164}, Bir 187 RC {ECO:0000313|EMBL:CKS41591.1, ECO:0000313|Proteomes:UP000049023}, RC G09801536 {ECO:0000313|EMBL:COW03350.1, RC ECO:0000313|Proteomes:UP000045842}, G09901357 RC {ECO:0000313|EMBL:CFE41166.1, ECO:0000313|Proteomes:UP000048289}, RC H09601792 {ECO:0000313|EMBL:CFE46976.1, RC ECO:0000313|Proteomes:UP000046947}, and P00601463 RC {ECO:0000313|EMBL:COW17773.1, ECO:0000313|Proteomes:UP000048600}; RG Pathogen Informatics; RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases. RN [4] {ECO:0000313|Proteomes:UP000041611, ECO:0000313|Proteomes:UP000046674} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=0351H {ECO:0000313|EMBL:CFH70051.1, RC ECO:0000313|Proteomes:UP000046674}, and M09400937 RC {ECO:0000313|EMBL:COV75687.1, ECO:0000313|Proteomes:UP000041611}; RG Pathogen Informatics; RA Murphy D.; RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases. RN [5] {ECO:0000313|EMBL:OMH59404.1, ECO:0000313|Proteomes:UP000189452} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=6548 {ECO:0000313|EMBL:OMH59404.1, RC ECO:0000313|Proteomes:UP000189452}; RA Bigi M., Bigi F., Soria M.A.; RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases. RN [6] {ECO:0000313|Proteomes:UP000256319, ECO:0000313|Proteomes:UP000257078} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=30994_S14 {ECO:0000313|EMBL:REM12503.1, RC ECO:0000313|Proteomes:UP000257078}, and T21_S20 RC {ECO:0000313|EMBL:REX48956.1, ECO:0000313|Proteomes:UP000256319}; RX PubMed=28099825; DOI=.1056/NEJMoa1604544; RA Shah N.S., Auld S.C., Brust J.C., Mathema B., Ismail N., Moodley P., RA Mlisana K., Allana S., Campbell A., Mthiyane T., Morris N., Mpangase P., RA van der Meulen H., Omar S.V., Brown T.S., Narechania A., Shaskina E., RA Kapwata T., Kreiswirth B., Gandhi N.R.; RT "Transmission of Extensively Drug-Resistant Tuberculosis in South Africa."; RL N. Engl. J. Med. 376:243-253(2017). RN [7] {ECO:0000313|EMBL:OMH59404.1, ECO:0000313|Proteomes:UP000189452} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=6548 {ECO:0000313|EMBL:OMH59404.1, RC ECO:0000313|Proteomes:UP000189452}; RA Bigi M.M., Lopez B., Blanco F.C., Sasiain M.C., De La Barrera S., RA Ritacco V., Bigi F., Soria M.A.; RT "Protein polymorphisms may explain contrasting epidemiological fitness of RT two variants of a multidrug-resistant Mycobacterium tuberculosis strain."; RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases. RN [8] {ECO:0000313|EMBL:REM12503.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=30994_S14 {ECO:0000313|EMBL:REM12503.1}, and T21_S20 RC {ECO:0000313|EMBL:REX48956.1}; RA Shah S., Brown T., Auld S., Bratton K., Narechania A., Mathema B., RA Gandhi N.; RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases. RN [9] {ECO:0000313|EMBL:VCU49751.1, ECO:0000313|Proteomes:UP000300237} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DKC2 {ECO:0000313|EMBL:VCU49751.1, RC ECO:0000313|Proteomes:UP000300237}; RA Fokvardsen B D., Norman A.; RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2,3-saturated acyl-CoA + NAD(+) = a (2E)-enoyl-CoA + H(+) + CC NADH; Xref=Rhea:RHEA:18177, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58856, ChEBI:CHEBI:65111; CC Evidence={ECO:0000256|ARBA:ARBA00001288}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:18179; CC Evidence={ECO:0000256|ARBA:ARBA00001288}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + CC H(+) + NADH; Xref=Rhea:RHEA:10240, Rhea:RHEA-COMP:9925, Rhea:RHEA- CC COMP:9926, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:78784, ChEBI:CHEBI:78785; EC=1.3.1.9; CC Evidence={ECO:0000256|ARBA:ARBA00000196}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:10242; CC Evidence={ECO:0000256|ARBA:ARBA00000196}; CC -!- PATHWAY: Lipid metabolism; mycolic acid biosynthesis. CC {ECO:0000256|ARBA:ARBA00004796}. CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) CC family. FabI subfamily. {ECO:0000256|ARBA:ARBA00009233, CC ECO:0000256|PIRNR:PIRNR000094}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KC692360; AGJ50654.1; -; Genomic_DNA. DR EMBL; KC692361; AGJ50655.1; -; Genomic_DNA. DR EMBL; KF563064; AHA90961.1; -; Genomic_DNA. DR EMBL; CFOE01000404; CFE41166.1; -; Genomic_DNA. DR EMBL; CFOH01000050; CFE46976.1; -; Genomic_DNA. DR EMBL; CFSW01000002; CFH70051.1; -; Genomic_DNA. DR EMBL; CNFT01000132; CKR17592.1; -; Genomic_DNA. DR EMBL; CNFU01000735; CKS41591.1; -; Genomic_DNA. DR EMBL; CSAT01000007; COV75687.1; -; Genomic_DNA. DR EMBL; CSAD01000466; COW03350.1; -; Genomic_DNA. DR EMBL; CHKL01000161; COW17773.1; -; Genomic_DNA. DR EMBL; LWDQ01000001; OMH59404.1; -; Genomic_DNA. DR EMBL; QSXC01000153; REM12503.1; -; Genomic_DNA. DR EMBL; QTHJ01000126; REX48956.1; -; Genomic_DNA. DR EMBL; LR027516; VCU49751.1; -; Genomic_DNA. DR RefSeq; WP_003407553.1; NZ_WUCS01000015.1. DR GeneID; 45425463; -. DR PATRIC; fig|1773.206.peg.1259; -. DR OMA; GILDMIH; -. DR UniPathway; UPA00915; -. DR Proteomes; UP000041611; Unassembled WGS sequence. DR Proteomes; UP000045842; Unassembled WGS sequence. DR Proteomes; UP000046674; Unassembled WGS sequence. DR Proteomes; UP000046947; Unassembled WGS sequence. DR Proteomes; UP000048289; Unassembled WGS sequence. DR Proteomes; UP000048600; Unassembled WGS sequence. DR Proteomes; UP000049023; Unassembled WGS sequence. DR Proteomes; UP000050164; Unassembled WGS sequence. DR Proteomes; UP000189452; Chromosome. DR Proteomes; UP000256319; Unassembled WGS sequence. DR Proteomes; UP000257078; Unassembled WGS sequence. DR Proteomes; UP000300237; Chromosome 1. DR GO; GO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NADH) activity; IEA:UniProtKB-EC. DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW. DR CDD; cd05372; ENR_SDR; 1. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1. DR InterPro; IPR014358; Enoyl-ACP_Rdtase_NADH. DR InterPro; IPR053410; Mycobact_enoyl-ACP_red. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR NCBIfam; NF040631; InhA; 1. DR PANTHER; PTHR43159; ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE; 1. DR PANTHER; PTHR43159:SF2; ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE [NADH] FABI; 1. DR Pfam; PF13561; adh_short_C2; 1. DR PIRSF; PIRSF000094; Enoyl-ACP_rdct; 1. DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1. PE 3: Inferred from homology; KW Fatty acid biosynthesis {ECO:0000256|PIRNR:PIRNR000094}; KW Fatty acid metabolism {ECO:0000256|PIRNR:PIRNR000094}; KW Lipid biosynthesis {ECO:0000256|PIRNR:PIRNR000094}; KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR000094}; KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|PIRNR:PIRNR000094}; KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002, KW ECO:0000256|PIRNR:PIRNR000094}. FT BINDING 14 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 20..21 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 64..65 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 95 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 165 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 194..198 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" SQ SEQUENCE 269 AA; 28528 MW; F161D6D6A631CA08 CRC64; MTGLLDGKRI LVSGIITDSS IAFHIARVAQ EQGAQLVLTG FDRLRLIQRI TDRLPAKAPL LELDVQNEEH LASLAGRVTE AIGAGNKLDG VVHSIGFMPQ TGMGINPFFD APYADVSKGI HISAYSYASM AKALLPIMNP GGSIVGMDFD PSRAMPAYNW MTVAKSALES VNRFVAREAG KYGVRSNLVA AGPIRTLAMS AIVGGALGEE AGAQIQLLEE GWDQRAPIGW NMKDATPVAK TVCALLSDWL PATTGDIIYA DGGAHTQLL //