ID M9TGV3_MYCTX Unreviewed; 269 AA. AC M9TGV3; DT 26-JUN-2013, integrated into UniProtKB/TrEMBL. DT 26-JUN-2013, sequence version 1. DT 02-DEC-2020, entry version 72. DE RecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH] {ECO:0000256|ARBA:ARBA00017755, ECO:0000256|PIRNR:PIRNR000094}; DE EC=1.3.1.9 {ECO:0000256|PIRNR:PIRNR000094}; GN Name=inhA {ECO:0000313|EMBL:AGJ50654.1}; GN Synonyms=fabI {ECO:0000313|EMBL:TGD81125.1}, inhA_1 GN {ECO:0000313|EMBL:QEX89265.1}; GN ORFNames=DSI38_15275 {ECO:0000313|EMBL:REM65104.1}, E5M52_03035 GN {ECO:0000313|EMBL:TGD81125.1}, ERS007663_02660 GN {ECO:0000313|EMBL:CNV22734.1}, ERS007670_02839 GN {ECO:0000313|EMBL:CNV27465.1}, ERS007679_02961 GN {ECO:0000313|EMBL:COW03350.1}, ERS007681_02786 GN {ECO:0000313|EMBL:CFE41166.1}, ERS007688_00531 GN {ECO:0000313|EMBL:CFE46976.1}, ERS007741_01725 GN {ECO:0000313|EMBL:COW17773.1}, ERS013471_01761 GN {ECO:0000313|EMBL:CNY49422.1}, ERS023446_02245 GN {ECO:0000313|EMBL:CNN12986.1}, ERS024276_00561 GN {ECO:0000313|EMBL:CKN85930.1}, ERS027659_00857 GN {ECO:0000313|EMBL:CKR17592.1}, ERS027661_03047 GN {ECO:0000313|EMBL:CKS41591.1}, ERS075361_00176 GN {ECO:0000313|EMBL:CNF98643.1}, ERS094182_01549 GN {ECO:0000313|EMBL:CRF26114.1}, F6W99_00036 GN {ECO:0000313|EMBL:QEX89265.1}, SAMEA2683035_01034 GN {ECO:0000313|EMBL:SGH39969.1}; OS Mycobacterium tuberculosis. OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; OC Mycobacterium; Mycobacterium tuberculosis complex. OX NCBI_TaxID=1773 {ECO:0000313|EMBL:AGJ50654.1}; RN [1] {ECO:0000313|EMBL:AGJ50654.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=TB11-111 {ECO:0000313|EMBL:AGJ50654.1}, and TB11-153 RC {ECO:0000313|EMBL:AGJ50655.1}; RA Daum L.T., Rodriguez J.D., Bhattacharyya S.; RT "Ion Torrent full-gene sequencing and phenotypic drug susceptibility RT testing (DST) confirm multi-drug resistance (MDR) Mycobacterium RT tuberculosis in the United States."; RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:AHA90961.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=MtbPL.K131 {ECO:0000313|EMBL:AHA90961.1}; RX PubMed=24855126; DOI=10.1093/jac/dku161; RA Jagielski T., Bakula Z., Roeske K., Kaminski M., Napiorkowska A., RA Augustynowicz-Kopec E., Zwolska Z., Bielecki J.; RT "Detection of mutations associated with isoniazid resistance in multidrug- RT resistant Mycobacterium tuberculosis clinical isolates."; RL J. Antimicrob. Chemother. 69:2369-2375(2014). RN [3] {ECO:0000313|EMBL:CNN12986.1, ECO:0000313|Proteomes:UP000039953} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=A70144_2 {ECO:0000313|EMBL:CNN12986.1, RC ECO:0000313|Proteomes:UP000039953}; RA Murphy D.; RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases. RN [4] {ECO:0000313|EMBL:SGH39969.1, ECO:0000313|Proteomes:UP000182946} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=0596Z {ECO:0000313|EMBL:CRF26114.1, RC ECO:0000313|Proteomes:UP000047893}, 2926STDY5723788 RC {ECO:0000313|EMBL:SGH39969.1, ECO:0000313|Proteomes:UP000182946}, RC 302235 {ECO:0000313|EMBL:CNF98643.1, RC ECO:0000313|Proteomes:UP000042440}, Bir 185 RC {ECO:0000313|EMBL:CKR17592.1, ECO:0000313|Proteomes:UP000050164}, Bir RC 187 {ECO:0000313|EMBL:CKS41591.1, ECO:0000313|Proteomes:UP000049023}, RC Bir 89 {ECO:0000313|EMBL:CKN85930.1, RC ECO:0000313|Proteomes:UP000049989}, D00000071 RC {ECO:0000313|EMBL:CNV22734.1, ECO:0000313|Proteomes:UP000046213}, RC E09701106 {ECO:0000313|EMBL:CNV27465.1, RC ECO:0000313|Proteomes:UP000041288}, G09801536 RC {ECO:0000313|EMBL:COW03350.1, ECO:0000313|Proteomes:UP000045842}, RC G09901357 {ECO:0000313|EMBL:CFE41166.1, RC ECO:0000313|Proteomes:UP000048289}, H09601792 RC {ECO:0000313|EMBL:CFE46976.1, ECO:0000313|Proteomes:UP000046947}, RC P00601463 {ECO:0000313|EMBL:COW17773.1, RC ECO:0000313|Proteomes:UP000048600}, and R09500613 RC {ECO:0000313|EMBL:CNY49422.1, ECO:0000313|Proteomes:UP000040993}; RG Pathogen Informatics; RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases. RN [5] {ECO:0000313|EMBL:REM65104.1, ECO:0000313|Proteomes:UP000256245} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=31012_S19 {ECO:0000313|EMBL:REM65104.1, RC ECO:0000313|Proteomes:UP000256245}; RX PubMed=28099825; DOI=.1056/NEJMoa1604544; RA Shah N.S., Auld S.C., Brust J.C., Mathema B., Ismail N., Moodley P., RA Mlisana K., Allana S., Campbell A., Mthiyane T., Morris N., Mpangase P., RA van der Meulen H., Omar S.V., Brown T.S., Narechania A., Shaskina E., RA Kapwata T., Kreiswirth B., Gandhi N.R.; RT "Transmission of Extensively Drug-Resistant Tuberculosis in South Africa."; RL N. Engl. J. Med. 376:243-253(2017). RN [6] {ECO:0000313|EMBL:REM65104.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=31012_S19 {ECO:0000313|EMBL:REM65104.1}; RA Shah S., Brown T., Auld S., Bratton K., Narechania A., Mathema B., RA Gandhi N.; RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases. RN [7] {ECO:0000313|EMBL:TGD81125.1, ECO:0000313|Proteomes:UP000297584} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=EKB53 {ECO:0000313|EMBL:TGD81125.1, RC ECO:0000313|Proteomes:UP000297584}; RA Danilenko V.N., Bekker O.B., Shur K.V., Maslov D.A., Zaychikova M.V., RA Zakharevich N.V.; RT "Draft Genome Sequences of four Mycobacterium tuberculosis isolates."; RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases. RN [8] {ECO:0000313|EMBL:QEX89265.1, ECO:0000313|Proteomes:UP000326088} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=L {ECO:0000313|EMBL:QEX89265.1, RC ECO:0000313|Proteomes:UP000326088}; RA Aung H.L.; RT "Complete genome sequence of a New Zealand Mycobacterium tuberculosis RT strain responsible for the ongoing transmission over the last 30 years."; RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2,3-saturated acyl-CoA + NAD(+) = a (2E)-enoyl-CoA + H(+) + CC NADH; Xref=Rhea:RHEA:18177, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58856, ChEBI:CHEBI:65111; CC Evidence={ECO:0000256|ARBA:ARBA00001288}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:18179; CC Evidence={ECO:0000256|ARBA:ARBA00001288}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + CC H(+) + NADH; Xref=Rhea:RHEA:10240, Rhea:RHEA-COMP:9925, Rhea:RHEA- CC COMP:9926, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:78784, ChEBI:CHEBI:78785; EC=1.3.1.9; CC Evidence={ECO:0000256|PIRNR:PIRNR000094}; CC -!- PATHWAY: Lipid metabolism; mycolic acid biosynthesis. CC {ECO:0000256|ARBA:ARBA00004796}. CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) CC family. FabI subfamily. {ECO:0000256|ARBA:ARBA00009233, CC ECO:0000256|PIRNR:PIRNR000094}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KC692360; AGJ50654.1; -; Genomic_DNA. DR EMBL; KC692361; AGJ50655.1; -; Genomic_DNA. DR EMBL; KF563064; AHA90961.1; -; Genomic_DNA. DR EMBL; CFOE01000404; CFE41166.1; -; Genomic_DNA. DR EMBL; CFOH01000050; CFE46976.1; -; Genomic_DNA. DR EMBL; CNCN01000080; CKN85930.1; -; Genomic_DNA. DR EMBL; CNFT01000132; CKR17592.1; -; Genomic_DNA. DR EMBL; CNFU01000735; CKS41591.1; -; Genomic_DNA. DR EMBL; CQAC01000001; CNF98643.1; -; Genomic_DNA. DR EMBL; CQGE01000047; CNN12986.1; -; Genomic_DNA. DR EMBL; CQPV01000436; CNV22734.1; -; Genomic_DNA. DR EMBL; CQPL01000668; CNV27465.1; -; Genomic_DNA. DR EMBL; CQTT01000039; CNY49422.1; -; Genomic_DNA. DR EMBL; CSAD01000466; COW03350.1; -; Genomic_DNA. DR EMBL; CHKL01000161; COW17773.1; -; Genomic_DNA. DR EMBL; CVKR01000009; CRF26114.1; -; Genomic_DNA. DR EMBL; CP044345; QEX89265.1; -; Genomic_DNA. DR EMBL; QSXJ01001014; REM65104.1; -; Genomic_DNA. DR EMBL; FPTZ01000005; SGH39969.1; -; Genomic_DNA. DR EMBL; SRLJ01000004; TGD81125.1; -; Genomic_DNA. DR RefSeq; WP_003407553.1; NZ_WUJD01000014.1. DR ChEMBL; CHEMBL3879823; -. DR EnsemblBacteria; AMC45863; AMC45863; RN05_1587. DR EnsemblBacteria; CFE41166; CFE41166; ERS007681_02786. DR EnsemblBacteria; CFE46976; CFE46976; ERS007688_00531. DR EnsemblBacteria; CKN85930; CKN85930; ERS024276_00561. DR EnsemblBacteria; CKR15684; CKR15684; ERS027654_00753. DR EnsemblBacteria; CKR17592; CKR17592; ERS027659_00857. DR EnsemblBacteria; CKR40504; CKR40504; ERS027644_01157. DR EnsemblBacteria; CKR62543; CKR62543; ERS027656_01728. DR EnsemblBacteria; CKS36630; CKS36630; ERS027652_03095. DR EnsemblBacteria; CKS39180; CKS39180; ERS027653_02937. DR EnsemblBacteria; CKS41591; CKS41591; ERS027661_03047. DR EnsemblBacteria; CKS53350; CKS53350; ERS027666_01181. DR EnsemblBacteria; CKV85791; CKV85791; ERS031537_03411. DR EnsemblBacteria; CNG10554; CNG10554; ERS124361_00179. DR EnsemblBacteria; CNN12986; CNN12986; ERS023446_02245. DR EnsemblBacteria; CNV22734; CNV22734; ERS007663_02660. DR EnsemblBacteria; CNV27465; CNV27465; ERS007670_02839. DR EnsemblBacteria; CNY49422; CNY49422; ERS013471_01761. DR EnsemblBacteria; COW03350; COW03350; ERS007679_02961. DR EnsemblBacteria; COW17773; COW17773; ERS007741_01725. DR EnsemblBacteria; CRH12375; CRH12375; BN1303_01560. DR EnsemblBacteria; CRH14939; CRH14939; BN1213_03046. DR GeneID; 23492354; -. DR PATRIC; fig|1773.206.peg.1259; -. DR UniPathway; UPA00915; -. DR Proteomes; UP000039953; Unassembled WGS sequence. DR Proteomes; UP000040993; Unassembled WGS sequence. DR Proteomes; UP000041288; Unassembled WGS sequence. DR Proteomes; UP000042440; Unassembled WGS sequence. DR Proteomes; UP000045842; Unassembled WGS sequence. DR Proteomes; UP000046213; Unassembled WGS sequence. DR Proteomes; UP000046947; Unassembled WGS sequence. DR Proteomes; UP000047893; Unassembled WGS sequence. DR Proteomes; UP000048289; Unassembled WGS sequence. DR Proteomes; UP000048600; Unassembled WGS sequence. DR Proteomes; UP000049023; Unassembled WGS sequence. DR Proteomes; UP000049989; Unassembled WGS sequence. DR Proteomes; UP000050164; Unassembled WGS sequence. DR Proteomes; UP000182946; Unassembled WGS sequence. DR Proteomes; UP000256245; Unassembled WGS sequence. DR Proteomes; UP000297584; Unassembled WGS sequence. DR Proteomes; UP000326088; Chromosome. DR GO; GO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NADH) activity; IEA:UniProtKB-EC. DR GO; GO:0016631; F:enoyl-[acyl-carrier-protein] reductase activity; IEA:UniProtKB-EC. DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW. DR CDD; cd05372; ENR_SDR; 1. DR InterPro; IPR014358; Enoyl-ACP_Rdtase_NADH. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR PANTHER; PTHR43159:SF2; PTHR43159:SF2; 1. DR PIRSF; PIRSF000094; Enoyl-ACP_rdct; 1. DR SUPFAM; SSF51735; SSF51735; 1. PE 3: Inferred from homology; KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160, KW ECO:0000256|PIRNR:PIRNR000094}; KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00023160, KW ECO:0000256|PIRNR:PIRNR000094}; KW Lipid biosynthesis {ECO:0000256|PIRNR:PIRNR000094}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023160, KW ECO:0000256|PIRNR:PIRNR000094}; KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|PIRNR:PIRNR000094, KW ECO:0000256|PIRSR:PIRSR000094-3}; KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002, KW ECO:0000256|PIRNR:PIRNR000094, ECO:0000313|EMBL:CFE41166.1}. FT NP_BIND 20..21 FT /note="NAD" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT NP_BIND 64..65 FT /note="NAD" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT NP_BIND 194..198 FT /note="NAD" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 14 FT /note="NAD; via carbonyl oxygen" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 95 FT /note="NAD; via carbonyl oxygen" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 165 FT /note="NAD" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" SQ SEQUENCE 269 AA; 28528 MW; F161D6D6A631CA08 CRC64; MTGLLDGKRI LVSGIITDSS IAFHIARVAQ EQGAQLVLTG FDRLRLIQRI TDRLPAKAPL LELDVQNEEH LASLAGRVTE AIGAGNKLDG VVHSIGFMPQ TGMGINPFFD APYADVSKGI HISAYSYASM AKALLPIMNP GGSIVGMDFD PSRAMPAYNW MTVAKSALES VNRFVAREAG KYGVRSNLVA AGPIRTLAMS AIVGGALGEE AGAQIQLLEE GWDQRAPIGW NMKDATPVAK TVCALLSDWL PATTGDIIYA DGGAHTQLL //