ID M9TGV3_MYCTX Unreviewed; 269 AA. AC M9TGV3; DT 26-JUN-2013, integrated into UniProtKB/TrEMBL. DT 26-JUN-2013, sequence version 1. DT 26-FEB-2020, entry version 67. DE RecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH] {ECO:0000256|PIRNR:PIRNR000094}; DE EC=1.3.1.9 {ECO:0000256|PIRNR:PIRNR000094}; GN Name=inhA {ECO:0000313|EMBL:AGJ50654.1}; GN Synonyms=fabI {ECO:0000313|EMBL:TGE07195.1}; GN ORFNames=E5M05_03060 {ECO:0000313|EMBL:TGE07195.1}, ERS023446_02245 GN {ECO:0000313|EMBL:CNN12986.1}, ERS027651_01123 GN {ECO:0000313|EMBL:CKR67679.1}, FCN16_08960 GN {ECO:0000313|EMBL:TKD74984.1}, SAMEA2682864_01868 GN {ECO:0000313|EMBL:SGM14702.1}, SAMEA2683035_01034 GN {ECO:0000313|EMBL:SGH39969.1}; OS Mycobacterium tuberculosis. OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; OC Mycobacterium; Mycobacterium tuberculosis complex. OX NCBI_TaxID=1773 {ECO:0000313|EMBL:AGJ50654.1}; RN [1] {ECO:0000313|EMBL:AGJ50654.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=TB11-111 {ECO:0000313|EMBL:AGJ50654.1}, and TB11-153 RC {ECO:0000313|EMBL:AGJ50655.1}; RA Daum L.T., Rodriguez J.D., Bhattacharyya S.; RT "Ion Torrent full-gene sequencing and phenotypic drug susceptibility RT testing (DST) confirm multi-drug resistance (MDR) Mycobacterium RT tuberculosis in the United States."; RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:AHA90961.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=MtbPL.K131 {ECO:0000313|EMBL:AHA90961.1}; RX PubMed=24855126; DOI=10.1093/jac/dku161; RA Jagielski T., Bakula Z., Roeske K., Kaminski M., Napiorkowska A., RA Augustynowicz-Kopec E., Zwolska Z., Bielecki J.; RT "Detection of mutations associated with isoniazid resistance in multidrug- RT resistant Mycobacterium tuberculosis clinical isolates."; RL J. Antimicrob. Chemother. 69:2369-2375(2014). RN [3] {ECO:0000313|EMBL:CNN12986.1, ECO:0000313|Proteomes:UP000039953} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=A70144_2 {ECO:0000313|EMBL:CNN12986.1, RC ECO:0000313|Proteomes:UP000039953}; RA Murphy D.; RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases. RN [4] {ECO:0000313|EMBL:SGH39969.1, ECO:0000313|Proteomes:UP000182946, ECO:0000313|Proteomes:UP000183655} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=2926STDY5723616 {ECO:0000313|EMBL:SGM14702.1, RC ECO:0000313|Proteomes:UP000183655}, 2926STDY5723788 RC {ECO:0000313|EMBL:SGH39969.1, ECO:0000313|Proteomes:UP000182946}, and RC Bir 177 {ECO:0000313|EMBL:CKR67679.1, RC ECO:0000313|Proteomes:UP000049058}; RG Pathogen Informatics; RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases. RN [5] {ECO:0000313|EMBL:TGE07195.1, ECO:0000313|Proteomes:UP000298175} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=EKB34 {ECO:0000313|EMBL:TGE07195.1, RC ECO:0000313|Proteomes:UP000298175}; RA Danilenko V.N., Bekker O.B., Shur K.V., Maslov D.A., Zaychikova M.V., RA Zakharevich N.V.; RT "Draft Genome Sequences of four Mycobacterium tuberculosis isolates."; RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases. RN [6] {ECO:0000313|EMBL:TKD74984.1, ECO:0000313|Proteomes:UP000310681} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=TBR019 {ECO:0000313|EMBL:TKD74984.1, RC ECO:0000313|Proteomes:UP000310681}; RA Solano-Gutierrez J.S., Robledo J., Rodriguez A.; RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + CC H(+) + NADH; Xref=Rhea:RHEA:10240, Rhea:RHEA-COMP:9925, Rhea:RHEA- CC COMP:9926, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:78784, ChEBI:CHEBI:78785; EC=1.3.1.9; CC Evidence={ECO:0000256|PIRNR:PIRNR000094}; CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) CC family. FabI subfamily. {ECO:0000256|PIRNR:PIRNR000094}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KC692360; AGJ50654.1; -; Genomic_DNA. DR EMBL; KC692361; AGJ50655.1; -; Genomic_DNA. DR EMBL; KF563064; AHA90961.1; -; Genomic_DNA. DR EMBL; CIEA01000007; CKR67679.1; -; Genomic_DNA. DR EMBL; CQGE01000047; CNN12986.1; -; Genomic_DNA. DR EMBL; FPTZ01000005; SGH39969.1; -; Genomic_DNA. DR EMBL; FPXX01000010; SGM14702.1; -; Genomic_DNA. DR EMBL; SRLI01000004; TGE07195.1; -; Genomic_DNA. DR EMBL; SWLC01000025; TKD74984.1; -; Genomic_DNA. DR RefSeq; WP_003407553.1; NZ_WFLD01000001.1. DR ChEMBL; CHEMBL3879823; -. DR EnsemblBacteria; AMC45863; AMC45863; RN05_1587. DR EnsemblBacteria; CFE41166; CFE41166; ERS007681_02786. DR EnsemblBacteria; CFE46976; CFE46976; ERS007688_00531. DR EnsemblBacteria; CKN85930; CKN85930; ERS024276_00561. DR EnsemblBacteria; CKR15684; CKR15684; ERS027654_00753. DR EnsemblBacteria; CKR17592; CKR17592; ERS027659_00857. DR EnsemblBacteria; CKR40504; CKR40504; ERS027644_01157. DR EnsemblBacteria; CKR62543; CKR62543; ERS027656_01728. DR EnsemblBacteria; CKS36630; CKS36630; ERS027652_03095. DR EnsemblBacteria; CKS39180; CKS39180; ERS027653_02937. DR EnsemblBacteria; CKS41591; CKS41591; ERS027661_03047. DR EnsemblBacteria; CKS53350; CKS53350; ERS027666_01181. DR EnsemblBacteria; CKV85791; CKV85791; ERS031537_03411. DR EnsemblBacteria; CNG10554; CNG10554; ERS124361_00179. DR EnsemblBacteria; CNN12986; CNN12986; ERS023446_02245. DR EnsemblBacteria; CNV22734; CNV22734; ERS007663_02660. DR EnsemblBacteria; CNV27465; CNV27465; ERS007670_02839. DR EnsemblBacteria; CNY49422; CNY49422; ERS013471_01761. DR EnsemblBacteria; COW03350; COW03350; ERS007679_02961. DR EnsemblBacteria; COW17773; COW17773; ERS007741_01725. DR EnsemblBacteria; CRH12375; CRH12375; BN1303_01560. DR EnsemblBacteria; CRH14939; CRH14939; BN1213_03046. DR PATRIC; fig|1773.206.peg.1259; -. DR eggNOG; ENOG4105CSJ; Bacteria. DR eggNOG; COG0623; LUCA. DR Proteomes; UP000039953; Unassembled WGS sequence. DR Proteomes; UP000049058; Unassembled WGS sequence. DR Proteomes; UP000182946; Unassembled WGS sequence. DR Proteomes; UP000183655; Unassembled WGS sequence. DR Proteomes; UP000298175; Unassembled WGS sequence. DR Proteomes; UP000310681; Unassembled WGS sequence. DR GO; GO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NADH) activity; IEA:UniProtKB-EC. DR GO; GO:0016631; F:enoyl-[acyl-carrier-protein] reductase activity; IEA:UniProtKB-EC. DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW. DR CDD; cd05372; ENR_SDR; 1. DR InterPro; IPR014358; Enoyl-ACP_Rdtase_NADH. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR PANTHER; PTHR43159:SF2; PTHR43159:SF2; 1. DR PIRSF; PIRSF000094; Enoyl-ACP_rdct; 1. DR SUPFAM; SSF51735; SSF51735; 1. PE 3: Inferred from homology; KW Fatty acid biosynthesis {ECO:0000256|PIRNR:PIRNR000094}; KW Fatty acid metabolism {ECO:0000256|PIRNR:PIRNR000094}; KW Lipid biosynthesis {ECO:0000256|PIRNR:PIRNR000094}; KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR000094}; KW NAD {ECO:0000256|PIRNR:PIRNR000094, ECO:0000256|PIRSR:PIRSR000094-3}; KW Oxidoreductase {ECO:0000256|PIRNR:PIRNR000094, KW ECO:0000313|EMBL:CKR67679.1}. FT NP_BIND 20..21 FT /note="NAD" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT NP_BIND 64..65 FT /note="NAD" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT NP_BIND 194..198 FT /note="NAD" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 14 FT /note="NAD; via carbonyl oxygen" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 95 FT /note="NAD; via carbonyl oxygen" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 165 FT /note="NAD" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" SQ SEQUENCE 269 AA; 28528 MW; F161D6D6A631CA08 CRC64; MTGLLDGKRI LVSGIITDSS IAFHIARVAQ EQGAQLVLTG FDRLRLIQRI TDRLPAKAPL LELDVQNEEH LASLAGRVTE AIGAGNKLDG VVHSIGFMPQ TGMGINPFFD APYADVSKGI HISAYSYASM AKALLPIMNP GGSIVGMDFD PSRAMPAYNW MTVAKSALES VNRFVAREAG KYGVRSNLVA AGPIRTLAMS AIVGGALGEE AGAQIQLLEE GWDQRAPIGW NMKDATPVAK TVCALLSDWL PATTGDIIYA DGGAHTQLL //