ID M9PFV9_DROME Unreviewed; 313 AA. AC M9PFV9; DT 26-JUN-2013, integrated into UniProtKB/TrEMBL. DT 26-JUN-2013, sequence version 1. DT 14-DEC-2022, entry version 64. DE RecName: Full=Transcription elongation factor {ECO:0000256|RuleBase:RU368078}; GN Name=TfIIS {ECO:0000313|EMBL:AGB92992.1, GN ECO:0000313|FlyBase:FBgn0010422}; GN Synonyms=BG:DS00929.12 {ECO:0000313|EMBL:AGB92992.1}, br52 GN {ECO:0000313|EMBL:AGB92992.1}, Dmel\CG3710 GN {ECO:0000313|EMBL:AGB92992.1}, DmS-II {ECO:0000313|EMBL:AGB92992.1}, GN DmSII {ECO:0000313|EMBL:AGB92992.1}, l(2)35Cf GN {ECO:0000313|EMBL:AGB92992.1}, l(2)35cF {ECO:0000313|EMBL:AGB92992.1}, GN l(2)br52 {ECO:0000313|EMBL:AGB92992.1}, l35Cf GN {ECO:0000313|EMBL:AGB92992.1}, RnpSII {ECO:0000313|EMBL:AGB92992.1}, GN TFIIS {ECO:0000313|EMBL:AGB92992.1}, TFIISA GN {ECO:0000313|EMBL:AGB92992.1}, TFS-II {ECO:0000313|EMBL:AGB92992.1}; GN ORFNames=CG3710 {ECO:0000313|EMBL:AGB92992.1, GN ECO:0000313|FlyBase:FBgn0010422}, Dmel_CG3710 GN {ECO:0000313|EMBL:AGB92992.1}; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; OC Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227 {ECO:0000313|EMBL:AGB92992.1, ECO:0000313|Proteomes:UP000000803}; RN [1] {ECO:0000313|EMBL:AGB92992.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., RA Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., RA Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A., RA An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D., RA Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F., RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., RA Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., RA Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., RA Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E., RA Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., RA Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A., RA Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C., RA Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G., RA Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S., RA Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [2] {ECO:0000313|EMBL:AGB92992.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537568; RA Celniker S.E., Wheeler D.A., Kronmiller B., Carlson J.W., Halpern A., RA Patel S., Adams M., Champe M., Dugan S.P., Frise E., Hodgson A., RA George R.A., Hoskins R.A., Laverty T., Muzny D.M., Nelson C.R., RA Pacleb J.M., Park S., Pfeiffer B.D., Richards S., Sodergren E.J., RA Svirskas R., Tabor P.E., Wan K., Stapleton M., Sutton G.G., Venter C., RA Weinstock G., Scherer S.E., Myers E.W., Gibbs R.A., Rubin G.M.; RT "Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster RT euchromatic genome sequence."; RL Genome Biol. 3:RESEARCH0079-RESEARCH0079(2002). RN [3] {ECO:0000313|EMBL:AGB92992.1, ECO:0000313|Proteomes:UP000000803} RP GENOME REANNOTATION. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfied E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D., Drysdale R.A., Harris N.L., RA Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., RA Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic RT review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [4] {ECO:0000313|EMBL:AGB92992.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537573; RA Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R., RA Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M., RA Celniker S.E.; RT "The transposable elements of the Drosophila melanogaster euchromatin: a RT genomics perspective."; RL Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002). RN [5] {ECO:0000313|EMBL:AGB92992.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537574; RA Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A., RA Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G., RA Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M., RA Karpen G.H.; RT "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly."; RL Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002). RN [6] {ECO:0000313|EMBL:AGB92992.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=16110336; DOI=10.1371/journal.pcbi.0010022; RA Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D., RA Ashburner M., Anxolabehere D.; RT "Combined evidence annotation of transposable elements in genome RT sequences."; RL PLoS Comput. Biol. 1:166-175(2005). RN [7] {ECO:0000313|EMBL:AGB92992.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=17569856; DOI=10.1126/science.1139815; RA Smith C.D., Shu S., Mungall C.J., Karpen G.H.; RT "The Release 5.1 annotation of Drosophila melanogaster heterochromatin."; RL Science 316:1586-1591(2007). RN [8] {ECO:0000313|EMBL:AGB92992.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=17569867; DOI=10.1126/science.1139816; RA Hoskins R.A., Carlson J.W., Kennedy C., Acevedo D., Evans-Holm M., RA Frise E., Wan K.H., Park S., Mendez-Lago M., Rossi F., Villasante A., RA Dimitri P., Karpen G.H., Celniker S.E.; RT "Sequence finishing and mapping of Drosophila melanogaster RT heterochromatin."; RL Science 316:1625-1628(2007). CC -!- FUNCTION: Necessary for efficient RNA polymerase II transcription CC elongation past template-encoded arresting sites. The arresting sites CC in DNA have the property of trapping a certain fraction of elongating CC RNA polymerases that pass through, resulting in locked ternary CC complexes. Cleavage of the nascent transcript by S-II allows the CC resumption of elongation from the new 3'-terminus. CC {ECO:0000256|ARBA:ARBA00025408}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123, CC ECO:0000256|PROSITE-ProRule:PRU00649, ECO:0000256|RuleBase:RU368078}. CC -!- SIMILARITY: Belongs to the TFS-II family. CC {ECO:0000256|ARBA:ARBA00009647, ECO:0000256|RuleBase:RU368078}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE014134; AGB92992.1; -; Genomic_DNA. DR RefSeq; NP_001260457.1; NM_001273528.1. DR RefSeq; NP_476967.1; NM_057619.4. DR AlphaFoldDB; M9PFV9; -. DR SMR; M9PFV9; -. DR DNASU; 34883; -. DR EnsemblMetazoa; FBtr0080722; FBpp0080281; FBgn0010422. DR EnsemblMetazoa; FBtr0332475; FBpp0304751; FBgn0010422. DR GeneID; 34883; -. DR KEGG; dme:Dmel_CG3710; -. DR AGR; FB:FBgn0010422; -. DR CTD; 34883; -. DR FlyBase; FBgn0010422; TfIIS. DR VEuPathDB; VectorBase:FBgn0010422; -. DR HOGENOM; CLU_037637_2_0_1; -. DR OMA; KMVTRKS; -. DR OrthoDB; 1579101at2759; -. DR PhylomeDB; M9PFV9; -. DR BioGRID-ORCS; 34883; 1 hit in 3 CRISPR screens. DR GenomeRNAi; 34883; -. DR Proteomes; UP000000803; Chromosome 2L. DR Bgee; FBgn0010422; Expressed in embryonic/larval hemocyte (Drosophila) and 34 other tissues. DR ExpressionAtlas; M9PFV9; baseline and differential. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW. DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0006368; P:transcription elongation by RNA polymerase II promoter; IEA:InterPro. DR Gene3D; 1.10.472.30; -; 1. DR Gene3D; 1.20.930.10; -; 1. DR InterPro; IPR035100; TF_IIS-typ. DR InterPro; IPR003617; TFIIS/CRSP70_N_sub. DR InterPro; IPR035441; TFIIS/LEDGF_dom_sf. DR InterPro; IPR003618; TFIIS_cen_dom. DR InterPro; IPR036575; TFIIS_cen_dom_sf. DR InterPro; IPR017923; TFIIS_N. DR InterPro; IPR006289; TFSII. DR InterPro; IPR001222; Znf_TFIIS. DR Pfam; PF08711; Med26; 1. DR Pfam; PF01096; TFIIS_C; 1. DR Pfam; PF07500; TFIIS_M; 1. DR PIRSF; PIRSF006704; TF_IIS; 1. DR SMART; SM00510; TFS2M; 1. DR SMART; SM00509; TFS2N; 1. DR SMART; SM00440; ZnF_C2C2; 1. DR SUPFAM; SSF47676; Conserved domain common to transcription factors TFIIS, elongin A, CRSP70; 1. DR SUPFAM; SSF46942; Elongation factor TFIIS domain 2; 1. DR TIGRFAMs; TIGR01385; TFSII; 1. DR PROSITE; PS51321; TFIIS_CENTRAL; 1. DR PROSITE; PS51319; TFIIS_N; 1. DR PROSITE; PS00466; ZF_TFIIS_1; 1. DR PROSITE; PS51133; ZF_TFIIS_2; 1. PE 3: Inferred from homology; KW DNA-binding {ECO:0000256|RuleBase:RU368078}; KW Elongation factor {ECO:0000313|EMBL:AGB92992.1}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|RuleBase:RU368078}; KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PROSITE- KW ProRule:PRU00649}; Protein biosynthesis {ECO:0000313|EMBL:AGB92992.1}; KW Reference proteome {ECO:0000313|Proteomes:UP000000803}; KW Transcription {ECO:0000256|RuleBase:RU368078}; KW Transcription regulation {ECO:0000256|RuleBase:RU368078}; KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU368078}; KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE- KW ProRule:PRU00472}. FT DOMAIN 5..83 FT /note="TFIIS N-terminal" FT /evidence="ECO:0000259|PROSITE:PS51319" FT DOMAIN 153..268 FT /note="TFIIS central" FT /evidence="ECO:0000259|PROSITE:PS51321" FT DOMAIN 271..311 FT /note="TFIIS-type" FT /evidence="ECO:0000259|PROSITE:PS51133" FT REGION 83..150 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 83..123 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 313 AA; 34300 MW; 9DC7D99D71BD3DA3 CRC64; MSVEEEVFRI QKKMSKMASD GTGQDQALDL LKALQTLNIN LDILTKTRIG MTVNELRKSS KDDEVIALAK TLIKNWKRFL ASPAPTTPNN SSAKEGSSNN SSASKSTSAA KSSSSISGKD KSSSSSSSKD KEKKGSTSSS QTSFPSGGMT DAVRIKCREM LATALKIGEV PEGCGEPEEM AAELEDAIYS EFNNTDMKYK NRIRSRVANL KDPKNPGLRG NFMCGAVTAK QLAKMTPEEM ASDEMKKLRE KFVKEAINDA QLATVQGTKT DLLKCAKCKK RNCTYNQLQT RSADEPMTTF VMCNECGNRW KFC //