ID   L0CEG4_ECOLX            Unreviewed;       158 AA.
AC   L0CEG4;
DT   06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT   06-MAR-2013, sequence version 1.
DT   04-FEB-2015, entry version 13.
DE   RecName: Full=Adenylosuccinate synthetase {ECO:0000256|RuleBase:RU000520};
DE            EC=6.3.4.4 {ECO:0000256|RuleBase:RU000520};
DE   Flags: Fragment;
GN   Name=purA {ECO:0000313|EMBL:AGA13695.1};
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562 {ECO:0000313|EMBL:AGA13695.1};
RN   [1] {ECO:0000313|EMBL:AGA13695.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=3662 {ECO:0000313|EMBL:AGA13699.1}, 3665
RC   {ECO:0000313|EMBL:AGA13702.1}, 3677 {ECO:0000313|EMBL:AGA13704.1}, and
RC   3697 {ECO:0000313|EMBL:AGA13695.1};
RX   PubMed=22995280; DOI=10.1016/j.meegid.2012.09.003;
RA   Gonzalez-Gonzalez A., Sanchez-Reyes L.L., Delgado Sapien G.,
RA   Eguiarte L.E., Souza V.;
RT   "Hierarchical clustering of genetic diversity associated to different
RT   levels of mutation and recombination in Escherichia coli: A study
RT   based on Mexican isolates.";
RL   Infect. Genet. Evol. 13C:187-197(2012).
RN   [2] {ECO:0000313|EMBL:AGA13695.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=3662 {ECO:0000313|EMBL:AGA13699.1}, 3665
RC   {ECO:0000313|EMBL:AGA13702.1}, 3677 {ECO:0000313|EMBL:AGA13704.1}, and
RC   3697 {ECO:0000313|EMBL:AGA13695.1};
RA   Gonzalez A.;
RL   Submitted (MAR-2012) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EMBL:AIQ87494.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=PR54 {ECO:0000313|EMBL:AIQ87494.1}, and PR56
RC   {ECO:0000313|EMBL:AIQ87496.1};
RA   Rakov A.V., Wang X.-Y., Wang C.-X., Liu S.-L.;
RT   "Genetic boundaries delineating Escherichia coli into genetic clusters
RT   of bacteria.";
RL   Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays an important role in the de novo pathway of purine
CC       nucleotide biosynthesis. {ECO:0000256|RuleBase:RU000520}.
CC   -!- CATALYTIC ACTIVITY: GTP + IMP + L-aspartate = GDP + phosphate +
CC       N(6)-(1,2-dicarboxyethyl)-AMP. {ECO:0000256|RuleBase:RU000520}.
CC   -!- PATHWAY: Purine metabolism; AMP biosynthesis via de novo pathway;
CC       AMP from IMP: step 1/2. {ECO:0000256|RuleBase:RU000520}.
CC   -!- SIMILARITY: Belongs to the adenylosuccinate synthetase family.
CC       {ECO:0000256|RuleBase:RU000520}.
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DR   EMBL; JQ819070; AGA13695.1; -; Genomic_DNA.
DR   EMBL; JQ819074; AGA13699.1; -; Genomic_DNA.
DR   EMBL; JQ819077; AGA13702.1; -; Genomic_DNA.
DR   EMBL; JQ819079; AGA13704.1; -; Genomic_DNA.
DR   EMBL; KM072714; AIQ87494.1; -; Genomic_DNA.
DR   EMBL; KM072716; AIQ87496.1; -; Genomic_DNA.
DR   UniPathway; UPA00075; UER00335.
DR   GO; GO:0004019; F:adenylosuccinate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0044208; P:'de novo' AMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_00011; Adenylosucc_synth; 1.
DR   InterPro; IPR018220; Adenylosuccinate_synthase_AS.
DR   InterPro; IPR001114; Adenylosuccinate_synthetase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR11846; PTHR11846; 1.
DR   Pfam; PF00709; Adenylsucc_synt; 1.
DR   SMART; SM00788; Adenylsucc_synt; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00513; ADENYLOSUCCIN_SYN_2; 1.
PE   3: Inferred from homology;
KW   GTP-binding {ECO:0000256|RuleBase:RU000520};
KW   Ligase {ECO:0000256|RuleBase:RU000520};
KW   Magnesium {ECO:0000256|RuleBase:RU000520};
KW   Metal-binding {ECO:0000256|RuleBase:RU000520};
KW   Nucleotide-binding {ECO:0000256|RuleBase:RU000520};
KW   Purine biosynthesis {ECO:0000256|RuleBase:RU000520}.
FT   NON_TER       1      1       {ECO:0000313|EMBL:AGA13695.1}.
FT   NON_TER     158    158       {ECO:0000313|EMBL:AGA13695.1}.
SQ   SEQUENCE   158 AA;  17285 MW;  C4F7CC1C3092A0FD CRC64;
     NAREKARGAK AIGTTGRGIG PAYEDKVARR GLRVGDLFDK ETFAEKLKEV MEYHNFQLVN
     YYNAEAVDYQ KVLDDTMAVA DILTSMVVDV SDLLDQARQR GDFVMFEGAQ GTLLDIDHGT
     YPYVTSSNTT AGGVATGSGL GPRYVDYVLG ILKAYSTR
//