ID K9BVR5_ACIBA Unreviewed; 410 AA. AC K9BVR5; DT 06-FEB-2013, integrated into UniProtKB/TrEMBL. DT 06-FEB-2013, sequence version 1. DT 06-MAR-2013, entry version 2. DE SubName: Full=23S rRNA m2A2503 methyltransferase; DE EC=2.1.1.-; GN Name=rlmN; ORFNames=ACINWC487_0529; OS Acinetobacter baumannii WC-487. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Moraxellaceae; Acinetobacter; OC Acinetobacter calcoaceticus/baumannii complex. OX NCBI_TaxID=903917; RN [1] RP NUCLEOTIDE SEQUENCE. RC STRAIN=WC-487; RA Harkins D.M., Durkin A.S., Beck E., Fedorova N.B., Kim M., Onuska J., RA Radune D., DePew J., Koroleva G.I., Singh I., Chahine M.A., Cash D.M., RA Huang X.-Z., Nikolich M.P., Nierman W.C., Fouts D.E.; RL Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: 2 S-adenosyl-L-methionine + adenine(2503) in CC 23S rRNA = S-adenosyl-L-homocysteine + L-methionine + 5'- CC deoxyadenosine + 2-methyladenine(2503) in 23S rRNA. CC -!- CATALYTIC ACTIVITY: 2 S-adenosyl-L-methionine + adenine(37) in CC tRNA = S-adenosyl-L-homocysteine + L-methionine + 5'- CC deoxyadenosine + 2-methyladenine(37) in tRNA. CC -!- COFACTOR: Binds 1 4Fe-4S cluster. The cluster is coordinated with CC 3 cysteines and an exchangeable S-adenosyl-L-methionine (By CC similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AMZR01000073; EKU57720.1; -; Genomic_DNA. DR Gene3D; 3.20.20.70; -; 1. DR HAMAP; MF_01849; 23SrRNA_methyltr_N; 1; -. DR InterPro; IPR013785; Aldolase_TIM. DR InterPro; IPR006638; Elp3/MiaB/NifB. DR InterPro; IPR004383; rRNA_lsu_MTrfase_RlmN. DR InterPro; IPR007197; rSAM. DR Pfam; PF04055; Radical_SAM; 1. DR PIRSF; PIRSF006004; CHP00048; 1. DR SMART; SM00729; Elp3; 1. DR TIGRFAMs; TIGR00048; TIGR00048; 1. PE 3: Inferred from homology; KW 4Fe-4S; Cytoplasm; Disulfide bond; Iron; Iron-sulfur; Metal-binding; KW Methyltransferase; rRNA processing; S-adenosyl-L-methionine; KW Transferase; tRNA processing. SQ SEQUENCE 410 AA; 45756 MW; 2E98CD9213296A88 CRC64; MSSAVVVSSE NLDGQQQSSS TPASPAAEKV NLLGMSRAQL EKFFEDIGEK KFRAGQVMKW IHQYFVTDFA EMTNISGKLR AKLEQICEIK APEVVHRHYS KDGTRKWVFR VGEGSGSLVE TVLIPAEDKT GSRKTLCISS QVGCALDCSF CSTGKQGFQR DLTPDEIIGQ LWMANYSYME EVPVAERERS VTNVVMMGMG EPLLNYDAVL SSMHIMLDDF AYGMSKRRVT LSTSGVVPKI DQLAQDIDVA LAISLHAPND ELRNELVPIN KKYPLAQLIA ACQRYIAKDG NESARKHVTI EYVMLEGVND QPEHAQQLLK LLKNLPSKIN LIPFNPFPHA PYGRSSRNRI ISFQKTLSDA GFVCTIRQTR GDDIDAACGQ LVGQVADRTR RAEQWQKKVA QRQEILRTQG //