ID J3U664_BACTU Unreviewed; 195 AA. AC J3U664; DT 31-OCT-2012, integrated into UniProtKB/TrEMBL. DT 31-OCT-2012, sequence version 1. DT 29-SEP-2021, entry version 42. DE RecName: Full=Thymidine kinase {ECO:0000256|ARBA:ARBA00012118, ECO:0000256|HAMAP-Rule:MF_00124}; DE EC=2.7.1.21 {ECO:0000256|ARBA:ARBA00012118, ECO:0000256|HAMAP-Rule:MF_00124}; GN Name=tdk {ECO:0000256|HAMAP-Rule:MF_00124}; GN ORFNames=BTG_21735 {ECO:0000313|EMBL:AFQ17763.1}; OS Bacillus thuringiensis HD-771. OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; OC Bacillus cereus group. OX NCBI_TaxID=1218175 {ECO:0000313|EMBL:AFQ17763.1, ECO:0000313|Proteomes:UP000005259}; RN [1] {ECO:0000313|EMBL:AFQ17763.1, ECO:0000313|Proteomes:UP000005259} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=HD-771 {ECO:0000313|EMBL:AFQ17763.1, RC ECO:0000313|Proteomes:UP000005259}; RA Doggett N., Teshima H., Bruce D., Detter J.C., Johnson S.L., Han C.; RL Submitted (AUG-2012) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + thymidine = ADP + dTMP + H(+); Xref=Rhea:RHEA:19129, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17748, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:63528, ChEBI:CHEBI:456216; EC=2.7.1.21; CC Evidence={ECO:0000256|ARBA:ARBA00000506, ECO:0000256|HAMAP- CC Rule:MF_00124, ECO:0000256|RuleBase:RU000544}; CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00124}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00124}. CC -!- SIMILARITY: Belongs to the thymidine kinase family. CC {ECO:0000256|ARBA:ARBA00007587, ECO:0000256|HAMAP-Rule:MF_00124, CC ECO:0000256|RuleBase:RU004165}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP003752; AFQ17763.1; -; Genomic_DNA. DR RefSeq; WP_000280851.1; NC_018500.1. DR SMR; J3U664; -. DR EnsemblBacteria; AFQ17763; AFQ17763; BTG_21735. DR GeneID; 56445984; -. DR KEGG; bti:BTG_21735; -. DR PATRIC; fig|1218175.3.peg.4369; -. DR HOGENOM; CLU_064400_3_0_9; -. DR Proteomes; UP000005259; Chromosome. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule. DR GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-UniRule. DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW. DR Gene3D; 3.40.50.300; -; 1. DR HAMAP; MF_00124; Thymidine_kinase; 1. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR001267; Thymidine_kinase. DR InterPro; IPR020633; Thymidine_kinase_CS. DR PANTHER; PTHR11441; PTHR11441; 1. DR Pfam; PF00265; TK; 1. DR PIRSF; PIRSF035805; TK_cell; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR PROSITE; PS00603; TK_CELLULAR_TYPE; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP- KW Rule:MF_00124}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00124}; KW DNA synthesis {ECO:0000256|ARBA:ARBA00022634, ECO:0000256|HAMAP- KW Rule:MF_00124}; KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|HAMAP-Rule:MF_00124}; KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00124}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP- KW Rule:MF_00124}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP- KW Rule:MF_00124}; Zinc {ECO:0000256|HAMAP-Rule:MF_00124}. FT NP_BIND 15..22 FT /note="ATP" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00124" FT NP_BIND 88..91 FT /note="ATP" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00124" FT ACT_SITE 89 FT /note="Proton acceptor" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00124, FT ECO:0000256|PIRSR:PIRSR035805-1" FT METAL 145 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00124" FT METAL 148 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00124" FT METAL 183 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00124" FT METAL 186 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00124" SQ SEQUENCE 195 AA; 21702 MW; 6B421C91EF8D86F4 CRC64; MYLINQNGWI EVICGSMFSG KSEELIRRVR RTQFAKQHAI VFKPCIDNRY SEEDVVSHNG LKVKAVPVSA SKDIFEHITE DMDVIAIDEV QFFDGDIVEV VQVLANRGYR VIVAGLDQDF RGLPFGQVPQ LMAIAEHVTK LQAVCSACGS PASRTQRLID GEPAAFDDPI ILVGASESYE PRCRHCHAVP TNKDK //