ID J3U664_BACTU Unreviewed; 195 AA. AC J3U664; DT 31-OCT-2012, integrated into UniProtKB/TrEMBL. DT 31-OCT-2012, sequence version 1. DT 11-JUN-2014, entry version 12. DE RecName: Full=Thymidine kinase; DE EC=2.7.1.21; GN Name=tdk; ORFNames=BTG_21735; OS Bacillus thuringiensis HD-771. OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; OC Bacillus cereus group. OX NCBI_TaxID=1218175; RN [1] RP NUCLEOTIDE SEQUENCE. RC STRAIN=HD-771; RA Doggett N., Teshima H., Bruce D., Detter J.C., Johnson S.L., Han C.; RL Submitted (AUG-2012) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: ATP + thymidine = ADP + thymidine 5'- CC phosphate. CC -!- SUBUNIT: Homotetramer (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the thymidine kinase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP003752; AFQ17763.1; -; Genomic_DNA. DR RefSeq; YP_006605796.1; NC_018500.1. DR ProteinModelPortal; J3U664; -. DR EnsemblBacteria; AFQ17763; AFQ17763; BTG_21735. DR GeneID; 13493061; -. DR KEGG; bti:BTG_21735; -. DR KO; K00857; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP. DR GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-HAMAP. DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW. DR HAMAP; MF_00124; Thymidine_kinase; 1. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR001267; Thymidine_kinase. DR InterPro; IPR020633; Thymidine_kinase_CS. DR InterPro; IPR020634; Thymidine_kinase_subgr. DR PANTHER; PTHR11441; PTHR11441; 1. DR Pfam; PF00265; TK; 1. DR PIRSF; PIRSF035805; TK_cell; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR PROSITE; PS00603; TK_CELLULAR_TYPE; 1. PE 3: Inferred from homology; KW ATP-binding; Complete proteome; Cytoplasm; DNA synthesis; Kinase; KW Metal-binding; Nucleotide-binding; Transferase; Zinc. FT NP_BIND 15 22 ATP (By similarity){EA15}. FT NP_BIND 88 91 ATP (By similarity){EA15}. FT ACT_SITE 89 89 Proton acceptor (By similarity){EA15}. FT METAL 145 145 Zinc (By similarity){EA15}. FT METAL 148 148 Zinc (By similarity){EA15}. FT METAL 183 183 Zinc (By similarity){EA15}. FT METAL 186 186 Zinc (By similarity){EA15}. SQ SEQUENCE 195 AA; 21702 MW; 6B421C91EF8D86F4 CRC64; MYLINQNGWI EVICGSMFSG KSEELIRRVR RTQFAKQHAI VFKPCIDNRY SEEDVVSHNG LKVKAVPVSA SKDIFEHITE DMDVIAIDEV QFFDGDIVEV VQVLANRGYR VIVAGLDQDF RGLPFGQVPQ LMAIAEHVTK LQAVCSACGS PASRTQRLID GEPAAFDDPI ILVGASESYE PRCRHCHAVP TNKDK //