ID J3U2M4_BACTU Unreviewed; 211 AA. AC J3U2M4; DT 31-OCT-2012, integrated into UniProtKB/TrEMBL. DT 31-OCT-2012, sequence version 1. DT 16-OCT-2013, entry version 7. DE RecName: Full=FMN-dependent NADH-azoreductase; DE EC=1.7.-.-; DE AltName: Full=Azo-dye reductase; DE AltName: Full=FMN-dependent NADH-azo compound oxidoreductase; GN Name=azoR; ORFNames=BTG_08915; OS Bacillus thuringiensis HD-771. OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; OC Bacillus cereus group. OX NCBI_TaxID=1218175; RN [1] RP NUCLEOTIDE SEQUENCE. RC STRAIN=HD-771; RA Doggett N., Teshima H., Bruce D., Detter J.C., Johnson S.L., Han C.; RL Submitted (AUG-2012) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the reductive cleavage of azo bond in aromatic CC azo compounds to the corresponding amines. Requires NADH, but not CC NADPH, as an electron donor for its activity (By similarity). CC -!- COFACTOR: Binds 1 FMN per subunit (By similarity). CC -!- SUBUNIT: Homodimer (By similarity). CC -!- SIMILARITY: Belongs to the azoreductase type 1 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP003752; AFQ15253.1; -; Genomic_DNA. DR RefSeq; YP_006603286.1; NC_018500.1. DR ProteinModelPortal; J3U2M4; -. DR EnsemblBacteria; AFQ15253; AFQ15253; BTG_08915. DR GeneID; 13498220; -. DR KEGG; bti:BTG_08915; -. DR KO; K01118; -. DR GO; GO:0009055; F:electron carrier activity; IEA:UniProtKB-HAMAP. DR GO; GO:0010181; F:FMN binding; IEA:UniProtKB-HAMAP. DR GO; GO:0008752; F:FMN reductase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0016652; F:oxidoreductase activity, acting on NAD(P)H, NAD(P) as acceptor; IEA:UniProtKB-HAMAP. DR GO; GO:0016661; F:oxidoreductase activity, acting on other nitrogenous compounds as donors; IEA:InterPro. DR HAMAP; MF_01216; Azoreductase_type1; 1; -. DR InterPro; IPR003680; Flavodoxin_fold. DR InterPro; IPR023048; NADH-azoreductase_FMN-depdnt. DR PANTHER; PTHR10204:SF4; PTHR10204:SF4; 1. DR Pfam; PF02525; Flavodoxin_2; 1. PE 3: Inferred from homology; KW Flavoprotein; FMN; NAD; Oxidoreductase. SQ SEQUENCE 211 AA; 22984 MW; 2367909A8B0E0ACD CRC64; MTKVLFITAN PNSAEGSFGM AVGEAFIEAY KNEHPQDEVV TIDLFNTTVP AIDADVFGAW GKFAAGEGFE TLTEVQQQKV AAMNTNLETF MHADRYVFVT PMWNFSYPPV VKAYLDNVAI AGKTFKYTEN GPVGLLEGKK ALHIQATGGV YSEGAYAAVD FGRNHLKTVL GFVGVNETEY IAVEGMNANP EKAQEIKEAA IANARELAKR F //