ID J3U2M4_BACTU Unreviewed; 211 AA. AC J3U2M4; DT 31-OCT-2012, integrated into UniProtKB/TrEMBL. DT 31-OCT-2012, sequence version 1. DT 26-NOV-2014, entry version 12. DE RecName: Full=FMN-dependent NADH-azoreductase {ECO:0000256|HAMAP-Rule:MF_01216}; DE EC=1.7.-.- {ECO:0000256|HAMAP-Rule:MF_01216}; DE AltName: Full=Azo-dye reductase {ECO:0000256|HAMAP-Rule:MF_01216}; DE AltName: Full=FMN-dependent NADH-azo compound oxidoreductase {ECO:0000256|HAMAP-Rule:MF_01216}; GN Name=azoR {ECO:0000256|HAMAP-Rule:MF_01216}; GN ORFNames=BTG_08915 {ECO:0000313|EMBL:AFQ15253.1}; OS Bacillus thuringiensis HD-771. OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; OC Bacillus cereus group. OX NCBI_TaxID=1218175 {ECO:0000313|EMBL:AFQ15253.1, ECO:0000313|Proteomes:UP000005259}; RN [1] {ECO:0000313|EMBL:AFQ15253.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=HD-771 {ECO:0000313|EMBL:AFQ15253.1}; RA Doggett N., Teshima H., Bruce D., Detter J.C., Johnson S.L., Han C.; RL Submitted (AUG-2012) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the reductive cleavage of azo bond in aromatic CC azo compounds to the corresponding amines. Requires NADH, but not CC NADPH, as an electron donor for its activity. {ECO:0000256|HAMAP- CC Rule:MF_01216, ECO:0000256|SAAS:SAAS00016147}. CC -!- COFACTOR: CC Note=Binds 1 FMN per subunit. {ECO:0000256|HAMAP-Rule:MF_01216}; CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01216, CC ECO:0000256|SAAS:SAAS00016163}. CC -!- SIMILARITY: Belongs to the azoreductase type 1 family. CC {ECO:0000256|HAMAP-Rule:MF_01216}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP003752; AFQ15253.1; -; Genomic_DNA. DR RefSeq; YP_006603286.1; NC_018500.1. DR ProteinModelPortal; J3U2M4; -. DR EnsemblBacteria; AFQ15253; AFQ15253; BTG_08915. DR GeneID; 13498220; -. DR KEGG; bti:BTG_08915; -. DR KO; K01118; -. DR GO; GO:0009055; F:electron carrier activity; IEA:UniProtKB-HAMAP. DR GO; GO:0010181; F:FMN binding; IEA:UniProtKB-HAMAP. DR GO; GO:0008752; F:FMN reductase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0016652; F:oxidoreductase activity, acting on NAD(P)H, NAD(P) as acceptor; IEA:UniProtKB-HAMAP. DR GO; GO:0016661; F:oxidoreductase activity, acting on other nitrogenous compounds as donors; IEA:InterPro. DR Gene3D; 3.40.50.360; -; 1. DR HAMAP; MF_01216; Azoreductase_type1; 1. DR InterPro; IPR003680; Flavodoxin_fold. DR InterPro; IPR029039; Flavoprotein-like. DR InterPro; IPR023048; NADH-azoreductase_FMN-depdnt. DR PANTHER; PTHR10204:SF4; PTHR10204:SF4; 1. DR Pfam; PF02525; Flavodoxin_2; 1. DR SUPFAM; SSF52218; SSF52218; 1. PE 3: Inferred from homology; KW Complete proteome {ECO:0000313|Proteomes:UP000005259}; KW Flavoprotein {ECO:0000256|HAMAP-Rule:MF_01216, KW ECO:0000256|SAAS:SAAS00016150}; KW FMN {ECO:0000256|HAMAP-Rule:MF_01216, ECO:0000256|SAAS:SAAS00016179}; KW NAD {ECO:0000256|HAMAP-Rule:MF_01216, ECO:0000256|SAAS:SAAS00016149}; KW Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_01216, KW ECO:0000256|SAAS:SAAS00016167}. SQ SEQUENCE 211 AA; 22984 MW; 2367909A8B0E0ACD CRC64; MTKVLFITAN PNSAEGSFGM AVGEAFIEAY KNEHPQDEVV TIDLFNTTVP AIDADVFGAW GKFAAGEGFE TLTEVQQQKV AAMNTNLETF MHADRYVFVT PMWNFSYPPV VKAYLDNVAI AGKTFKYTEN GPVGLLEGKK ALHIQATGGV YSEGAYAAVD FGRNHLKTVL GFVGVNETEY IAVEGMNANP EKAQEIKEAA IANARELAKR F //