ID   I5ERD4_ECOLX            Unreviewed;       359 AA.
AC   I5ERD4;
DT   05-SEP-2012, integrated into UniProtKB/TrEMBL.
DT   05-SEP-2012, sequence version 1.
DT   26-NOV-2014, entry version 13.
DE   RecName: Full=TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase {ECO:0000256|HAMAP-Rule:MF_01002};
DE            EC=2.4.1.325 {ECO:0000256|HAMAP-Rule:MF_01002};
DE   AltName: Full=4-alpha-L-fucosyltransferase {ECO:0000256|HAMAP-Rule:MF_01002};
DE   AltName: Full=TDP-Fuc4NAc:lipid II Fuc4NAc transferase {ECO:0000256|HAMAP-Rule:MF_01002};
GN   Name=wecF {ECO:0000256|HAMAP-Rule:MF_01002,
GN   ECO:0000313|EMBL:EIN34493.1};
GN   Synonyms=rffT {ECO:0000256|HAMAP-Rule:MF_01002};
GN   ORFNames=EC93001_5147 {ECO:0000313|EMBL:EIN34493.1};
OS   Escherichia coli 93-001.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=1005481 {ECO:0000313|EMBL:EIN34493.1};
RN   [1] {ECO:0000313|EMBL:EIN34493.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=93-001 {ECO:0000313|EMBL:EIN34493.1};
RA   Eppinger M., Daugherty S., Agrawal S., Galens K., Tallon L.,
RA   Shefchek K., Parankush S., Cebula T.A., Feng P., Soderlund R.,
RA   Mammel M.K., DebRoy C., Dudley E.G., Tarr P.I., Fraser-Liggett C.,
RA   Ravel J.;
RT   "Genomic anatomy of Escherichia coli O157:H7 outbreaks.";
RL   Submitted (MAR-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA-Fuc4NAc
CC       (Lipid III), the third lipid-linked intermediate involved in ECA
CC       synthesis. {ECO:0000256|HAMAP-Rule:MF_01002,
CC       ECO:0000256|SAAS:SAAS00064577}.
CC   -!- CATALYTIC ACTIVITY: TDP-Fuc4NAc + Und-PP-GlcNAc-ManNAcA = TDP +
CC       Und-PP-GlcNAc-ManNAcA-Fuc4NAc. {ECO:0000256|SAAS:SAAS00064570}.
CC   -!- CATALYTIC ACTIVITY: dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
CC       + N-acetyl-beta-D-mannosaminouronyl-(1->4)-N-acetyl-alpha-D-
CC       glucosaminyl-diphospho-ditrans,octacis-undecaprenol = dTDP + 4-
CC       acetamido-4,6-dideoxy-alpha-D-galactosyl-(1->4)-N-acetyl-beta-D-
CC       mannosaminouronyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-
CC       ditrans,octacis-undecaprenol. {ECO:0000256|HAMAP-Rule:MF_01002}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial
CC       common antigen biosynthesis. {ECO:0000256|HAMAP-Rule:MF_01002,
CC       ECO:0000256|SAAS:SAAS00064581}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC       Rule:MF_01002, ECO:0000256|SAAS:SAAS00064580}; Peripheral membrane
CC       protein {ECO:0000256|HAMAP-Rule:MF_01002,
CC       ECO:0000256|SAAS:SAAS00064580}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 56 family.
CC       {ECO:0000256|HAMAP-Rule:MF_01002}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:EIN34493.1}.
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DR   EMBL; AKLB01000469; EIN34493.1; -; Genomic_DNA.
DR   ProteinModelPortal; I5ERD4; -.
DR   PRIDE; I5ERD4; -.
DR   EnsemblBacteria; EIN34493; EIN34493; EC93001_5147.
DR   UniPathway; UPA00566; -.
DR   GO; GO:0009276; C:Gram-negative-bacterium-type cell wall; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008417; F:fucosyltransferase activity; IEA:InterPro.
DR   GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_01002; WecF_RffT; 1.
DR   InterPro; IPR009993; 4-alpha-FUT.
DR   Pfam; PF07429; Glyco_transf_56; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_01002,
KW   ECO:0000256|SAAS:SAAS00064578};
KW   Cell membrane {ECO:0000256|HAMAP-Rule:MF_01002,
KW   ECO:0000256|SAAS:SAAS00064569};
KW   Glycosyltransferase {ECO:0000256|HAMAP-Rule:MF_01002,
KW   ECO:0000256|SAAS:SAAS00064575};
KW   Membrane {ECO:0000256|HAMAP-Rule:MF_01002,
KW   ECO:0000256|SAAS:SAAS00064573};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_01002,
KW   ECO:0000256|SAAS:SAAS00064568}.
SQ   SEQUENCE   359 AA;  40541 MW;  0510A64D395466D9 CRC64;
     MTVLIHVLGS DIPHHNRTVL RFFNDALAAT SEHAREFMVV GKDDGLSDSC PALSVQFFPG
     KKSLAEAVIA KAKANRQQRF FFHGQFNPTL WLALLSGGIK PSQFYWHIWG ADLYELSSGL
     RYKLFYPLRR LAQKRVGCVF ATRGDLSFFA KTHPKVRGEL LYFPTRMDAS LNTMANDRQR
     EGKMTILVGN SGDRSNEHIA ALRAVHQQFG DTVKVVVPMG YPPHNEAYIE EVRQAGLELF
     SEENLQVLSE KLEFDAYLTL LRQCALGYFI FARQQGIGTL CLLIQAGIPC VLNRENPFWQ
     DMTEQHLPVL FTTDDLNEDI VREAQRQLAS VDKNTIAFFS PNYLQGWQRA LAIAAGEVA
//