ID I3QIT9_PRRSV Unreviewed; 1460 AA. AC I3QIT9; DT 05-SEP-2012, integrated into UniProtKB/TrEMBL. DT 05-SEP-2012, sequence version 1. DT 29-SEP-2021, entry version 49. DE RecName: Full=Non-structural protein 8 {ECO:0000256|ARBA:ARBA00016245}; DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494}; DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552}; DE AltName: Full=ORF1ab polyprotein {ECO:0000256|ARBA:ARBA00015878}; DE AltName: Full=RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022412}; DE AltName: Full=Replicase polyprotein 1ab {ECO:0000256|ARBA:ARBA00022087}; DE Flags: Fragment; OS Porcine reproductive and respiratory syndrome virus (PRRSV). OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; OC Nidovirales; Arnidovirineae; Arteriviridae; unclassified Arteriviridae. OX NCBI_TaxID=28344 {ECO:0000313|EMBL:AFK09076.1, ECO:0000313|Proteomes:UP000107649}; OH NCBI_TaxID=9823; Sus scrofa (Pig). RN [1] {ECO:0000313|EMBL:AFK09076.1, ECO:0000313|Proteomes:UP000107649} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=10-10HEB-1 {ECO:0000313|EMBL:AFK09076.1}; RX PubMed=22525010; DOI=10.1016/j.vetmic.2012.02.036; RA Yu X., Chen N., Wang L., Wu J., Zhou Z., Ni J., Li X., Zhai X., Shi J., RA Tian K.; RT "New genomic characteristics of highly pathogenic porcine reproductive and RT respiratory syndrome viruses do not lead to significant changes in RT pathogenicity."; RL Vet. Microbiol. 158:291-299(2012). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JQ663551; AFK09076.1; -; Genomic_RNA. DR Proteomes; UP000107649; Genome. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC. DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR CDD; cd21410; 1B_av_Nsp10-like; 1. DR CDD; cd21160; NendoU_av_Nsp11-like; 1. DR CDD; cd21166; NTD_av_Nsp11-like; 1. DR CDD; cd21405; ZBD_av_Nsp10-like; 1. DR Gene3D; 3.40.50.300; -; 2. DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom. DR InterPro; IPR043502; DNA/RNA_pol_sf. DR InterPro; IPR037227; EndoU-like. DR InterPro; IPR043609; NendoU_nidovirus. DR InterPro; IPR044348; NSP10_1B_Av. DR InterPro; IPR027355; NSP10_Av_ZBD. DR InterPro; IPR044320; NSP11_Av_N. DR InterPro; IPR044314; NSP11_NendoU_Av. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR001205; RNA-dir_pol_C. DR InterPro; IPR007094; RNA-dir_pol_PSvirus. DR Pfam; PF19215; CoV_NSP15_C; 1. DR Pfam; PF00680; RdRP_1; 1. DR Pfam; PF01443; Viral_helicase1; 1. DR SUPFAM; SSF142877; SSF142877; 1. DR SUPFAM; SSF52540; SSF52540; 2. DR SUPFAM; SSF56672; SSF56672; 1. DR PROSITE; PS51652; AV_ZBD; 1. DR PROSITE; PS51657; PSRV_HELICASE; 1. DR PROSITE; PS50507; RDRP_SSRNA_POS; 1. PE 4: Predicted; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE- KW ProRule:PRU00985}; Nucleotide-binding {ECO:0000256|ARBA:ARBA00022840}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695}; KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}; KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}; KW Zinc {ECO:0000256|ARBA:ARBA00022833}; KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE- KW ProRule:PRU00985}. FT DOMAIN 389..523 FT /note="RdRp catalytic" FT /evidence="ECO:0000259|PROSITE:PS50507" FT DOMAIN 644..707 FT /note="AV ZBD" FT /evidence="ECO:0000259|PROSITE:PS51652" FT DOMAIN 764..1045 FT /note="(+)RNA virus helicase C-terminal" FT /evidence="ECO:0000259|PROSITE:PS51657" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:AFK09076.1" SQ SEQUENCE 1460 AA; 161442 MW; A8D03135FE54E505 CRC64; FKLLAASGLT RCGRGGLVVT ETAVKIVKFH NRTFTLGPVN LKVASEVELK DAVEHNQHPV ARPVDGGVVL LRSAVPSLID VLISGADASP KLLARHGPGN TGIDGTLWDF EAEATKEEVA LSAQIIQACD IRRGDAPEIG LPYKLHPVRG NPERVKGVLQ NTRFGDIPYK TPSDTGSPVH AAACLTPNAT PVTDGRSVLA TTMPSGFELY VPTIPASVLD YLDSRPDCPK QLTEHGCEDA ALRDLSKYDL STQGFVLPGV LRLVRKYLFA HVGKCPPVHR PSTYPAKNSM AGINGNRFPT KDIQSVPEID VLCAQAVREN WQTVTPCTLK KQYCGKKKTR TILGTNNFIA LAHRAALSGV TQGFMKKAFN SPIALGKNKF KELQAPVLGR CLEADLASCD RSTPAIVRWF AANLLYELAC AEEHLPSYVL NCCHDLLVTQ SGAVTKRGGL SSGDPITSVS NTIYSLVIYA QHMVLSYFKS GHPHGLLFLQ DQLKFEDMLK VQPLIVYSDD LVLYAESPSM PNYHWWVEHL NLMLGFQTDP KKTTITDSPS FLGCRIINGR QLVPNRDRIL AALAYHMKAS NVSEYYASAA AILMDSCACL EYDPEWFEEL VVGIAQCARK DGYSFPGPPF FLSMWEKLRS NHEGKKSRMC GYCGAPAPYA TACGLDVCVY HTHFHQHCPV IIWCGHPAGS GSCSECEPPL GKGTSPLDEV LEQVPYKPPR TVIMHVEQGL TPLDPGRYQT RRGLVSVRRG IKGNEVDLPD GDYASTALLP TCKEINMVAV ASNVLRSRFI IGPPGAGKTH WLLQQVQDGD VIYTPTHQTM LDMIRALGTC RFNVPAGTTL QFPAPSRTGP WVRILAGGWC PGKNSFLDEA AYCNHLDVLR LLSKTTLTCL GDFKQLHPVG FDSHCYVFDI MPQTQLKTIW RFGQNICDAI QPDYRDKLMS MVNTTRVTYV EKPVRYGQVL TPYHRDREDG AITIDSSQGA TFDVVTLHLP TKDSLNRQRA LVAITRARHA IFVYDPHRQL QSMFDLPAKG TPVNLAVHRD EQLIVLDRNN REITVAQALG NGDKFRATDK RVVDSLRAIC ADLEGSSSPL PKVAHNLGFY FSPDLTQFAK LPAELAPHWP VVTAQNNERW PDRLVASLRP IHKYSRACIG AGYMVGPSVF LGTPGVVSYY LTKFVRGEAQ VLPETVFSTG RIEVDCREYL DDREREVAES LPHAFIGDVK GTTVGGCHHV TSKYLPRFLP KESVAVVGVS SPGKAAKAVC TLTDVYLPDL EAYLHPETQS RCWKVMLDFK EVRLMVWKDK TAYFQLEGRH FTWYQLASYA SYIRVPVNST VYLDPCMGPA LCNRKVVGST HWGADLAVTP YDYGAKIILS SAYHGEMPPG CKILACAEFS LDDPVRYKHT WGFESDTAYL YEFTGNGEDW EDYNDAFRAR QKGKIYKANA TSMRFHFPPG PVIEPTLGLN //