ID I3JF34_ORENI Unreviewed; 276 AA. AC I3JF34; DT 11-JUL-2012, integrated into UniProtKB/TrEMBL. DT 11-JUL-2012, sequence version 1. DT 29-MAY-2024, entry version 59. DE RecName: Full=Insulin-induced gene protein {ECO:0000256|RuleBase:RU241113}; GN Name=insig1 {ECO:0000313|Ensembl:ENSONIP00000007475.1}; OS Oreochromis niloticus (Nile tilapia) (Tilapia nilotica). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata; OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids; OC Pseudocrenilabrinae; Oreochromini; Oreochromis. OX NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000007475.1, ECO:0000313|Proteomes:UP000005207}; RN [1] {ECO:0000313|Proteomes:UP000005207} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RG Broad Institute Genome Assembly Team; RG Broad Institute Sequencing Platform; RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.; RT "The Genome Sequence of Oreochromis niloticus (Nile Tilapia)."; RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|Ensembl:ENSONIP00000007475.1} RP IDENTIFICATION. RG Ensembl; RL Submitted (FEB-2024) to UniProtKB. CC -!- FUNCTION: Mediates feedback control of cholesterol synthesis. CC {ECO:0000256|RuleBase:RU241113}. CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane CC {ECO:0000256|ARBA:ARBA00004477}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004477}. Membrane CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004141}. CC -!- SIMILARITY: Belongs to the INSIG family. CC {ECO:0000256|ARBA:ARBA00007475, ECO:0000256|RuleBase:RU241113}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of CC feature annotation. {ECO:0000256|RuleBase:RU241113}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR RefSeq; XP_005448977.1; XM_005448920.3. DR AlphaFoldDB; I3JF34; -. DR STRING; 8128.ENSONIP00000007475; -. DR Ensembl; ENSONIT00000007480.2; ENSONIP00000007475.1; ENSONIG00000005939.2. DR GeneID; 100691598; -. DR CTD; 3638; -. DR eggNOG; KOG4363; Eukaryota. DR GeneTree; ENSGT00580000081600; -. DR HOGENOM; CLU_092922_0_0_1; -. DR InParanoid; I3JF34; -. DR OMA; FWSCSCT; -. DR OrthoDB; 2943928at2759; -. DR TreeFam; TF331013; -. DR Proteomes; UP000005207; Linkage group LG9. DR GO; GO:0032937; C:SREBP-SCAP-Insig complex; IEA:TreeGrafter. DR GO; GO:0032869; P:cellular response to insulin stimulus; IEA:TreeGrafter. DR GO; GO:0006695; P:cholesterol biosynthetic process; IEA:TreeGrafter. DR GO; GO:0032933; P:SREBP signaling pathway; IEA:TreeGrafter. DR GO; GO:0036316; P:SREBP-SCAP complex retention in endoplasmic reticulum; IEA:TreeGrafter. DR InterPro; IPR025929; INSIG_fam. DR PANTHER; PTHR15301; INSULIN-INDUCED GENE 1; 1. DR PANTHER; PTHR15301:SF11; INSULIN-INDUCED GENE 1 PROTEIN; 1. DR Pfam; PF07281; INSIG; 1. PE 3: Inferred from homology; KW Cholesterol metabolism {ECO:0000256|ARBA:ARBA00022548, KW ECO:0000256|RuleBase:RU241113}; KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824}; KW Lipid metabolism {ECO:0000256|RuleBase:RU241113}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU241113}; KW Reference proteome {ECO:0000313|Proteomes:UP000005207}; KW Steroid metabolism {ECO:0000256|RuleBase:RU241113}; KW Sterol metabolism {ECO:0000256|ARBA:ARBA00023166}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, KW ECO:0000256|RuleBase:RU241113}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|RuleBase:RU241113}. FT TRANSMEM 88..106 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU241113" FT TRANSMEM 126..146 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU241113" FT TRANSMEM 158..178 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU241113" FT TRANSMEM 184..203 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU241113" FT TRANSMEM 210..229 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU241113" FT TRANSMEM 241..262 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU241113" SQ SEQUENCE 276 AA; 30621 MW; 70398F796466DC4D CRC64; MNRRRGFCLE LTLKTSGDQA LKCQMPRLEE HCWSCSTSSK TETKHPSSGA NWLASRAEEM MSIITSVLSS AYISLHDVRT ANLIRRGVVL FTVGVFLALV LNLLQIQRNV TLFPEEVMTT LFSSAWWIPP CCGTGAAVIG LLYPCLDSHL GEPHKFKREW ASVMRCIAVF VGINHASVKL DFDNNVQLSL TLAALSLGLW WTFDRSRSGF GLGITTAFLA TVITQLLVYN GVYQYTSPDF LYVRSWLPCI FFSGGVTVGN IGRQLAMGGV EKPHMD //