ID I1PKS8_ORYGL Unreviewed; 114 AA. AC I1PKS8; DT 13-JUN-2012, integrated into UniProtKB/TrEMBL. DT 13-JUN-2012, sequence version 1. DT 11-DEC-2019, entry version 32. DE RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 {ECO:0000256|RuleBase:RU361136}; DE Short=Oligosaccharyl transferase subunit DAD1 {ECO:0000256|RuleBase:RU361136}; OS Oryza glaberrima (African rice). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; OC Oryzoideae; Oryzeae; Oryzinae; Oryza. OX NCBI_TaxID=4538 {ECO:0000313|EnsemblPlants:ORGLA04G0079700.1, ECO:0000313|Proteomes:UP000007306}; RN [1] {ECO:0000313|EnsemblPlants:ORGLA04G0079700.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=IRGC 96717 {ECO:0000313|EnsemblPlants:ORGLA04G0079700.1}; RA Wing R.A., Yu Y., Rounsley S., Reddy-Marri P., Goicoechea J.L., RA Sisneros N., Lee S., Song X., Angelova A., Kudrna D.P., de Baynast K., RA Zuccolo A.; RT "The complete genome of Oryza glaberrima."; RL Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EnsemblPlants:ORGLA04G0079700.1} RP IDENTIFICATION. RG EnsemblPlants; RL Submitted (JUN-2015) to UniProtKB. CC -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that CC catalyzes the initial transfer of a defined glycan CC (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- CC pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr CC consensus motif in nascent polypeptide chains, the first step in CC protein N-glycosylation. N-glycosylation occurs cotranslationally and CC the complex associates with the Sec61 complex at the channel-forming CC translocon complex that mediates protein translocation across the CC endoplasmic reticulum (ER). All subunits are required for a maximal CC enzyme activity. {ECO:0000256|RuleBase:RU361136}. CC -!- PATHWAY: Protein modification; protein glycosylation. CC {ECO:0000256|RuleBase:RU361136}. CC -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex. CC {ECO:0000256|RuleBase:RU361136}. CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane CC {ECO:0000256|RuleBase:RU361136}; Multi-pass membrane protein CC {ECO:0000256|RuleBase:RU361136}. CC -!- SIMILARITY: Belongs to the DAD/OST2 family. CC {ECO:0000256|RuleBase:RU361136}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR STRING; 4538.ORGLA04G0079700.1; -. DR EnsemblPlants; ORGLA02G0350300.1; ORGLA02G0350300.1; ORGLA02G0350300. DR EnsemblPlants; ORGLA04G0079700.1; ORGLA04G0079700.1; ORGLA04G0079700. DR Gramene; ORGLA02G0350300.1; ORGLA02G0350300.1; ORGLA02G0350300. DR Gramene; ORGLA04G0079700.1; ORGLA04G0079700.1; ORGLA04G0079700. DR eggNOG; KOG1746; Eukaryota. DR eggNOG; ENOG4111Q2I; LUCA. DR OMA; GVCLRLQ; -. DR UniPathway; UPA00378; -. DR Proteomes; UP000007306; Unassembled WGS sequence. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0008250; C:oligosaccharyltransferase complex; IEA:InterPro. DR GO; GO:0004579; F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; IEA:InterPro. DR GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway. DR InterPro; IPR003038; DAD/Ost2. DR PANTHER; PTHR10705; PTHR10705; 1. DR Pfam; PF02109; DAD; 1. DR PIRSF; PIRSF005588; DAD; 1. PE 3: Inferred from homology; KW Endoplasmic reticulum {ECO:0000256|RuleBase:RU361136}; KW Membrane {ECO:0000256|RuleBase:RU361136}; KW Reference proteome {ECO:0000313|Proteomes:UP000007306}; KW Transmembrane {ECO:0000256|RuleBase:RU361136}; KW Transmembrane helix {ECO:0000256|RuleBase:RU361136}. FT TRANSMEM 31..49 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361136" FT TRANSMEM 55..75 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361136" FT TRANSMEM 95..113 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361136" SQ SEQUENCE 114 AA; 12379 MW; 0BE69A7E935FA301 CRC64; MPRATSDAKL LIQSLGKAYA ATPTNLKIID LYVVFAVATA LIQVVYMGIV GSFPFNSFLS GVLSCIGTAV LAVCLRIQVN KDNKEFKDLP PERAFADFVL CNLVLHLVIM NFLG //