ID H9ZGM0_9PSED Unreviewed; 395 AA. AC H9ZGM0; DT 13-JUN-2012, integrated into UniProtKB/TrEMBL. DT 13-JUN-2012, sequence version 1. DT 03-MAY-2023, entry version 31. DE RecName: Full=DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00012418}; DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418}; DE Flags: Fragment; GN Name=rpoB {ECO:0000313|EMBL:AFH35126.1}; OS Pseudomonas sp. DOC21. OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=1170393 {ECO:0000313|EMBL:AFH35126.1}; RN [1] {ECO:0000313|EMBL:AFH35126.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=DOC21 {ECO:0000313|EMBL:AFH35126.1}; RX PubMed=22406861; DOI=10.1007/s00253-012-3966-7; RA Merino E., Barrientos A., Rodriguez J., Naharro G., Luengo J.M., RA Olivera E.R.; RT "Isolation of cholesterol- and deoxycholate-degrading bacteria from soil RT samples: evidence of a common pathway."; RL Appl. Microbiol. Biotechnol. 97:891-904(2013). CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550}; CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family. CC {ECO:0000256|RuleBase:RU000434}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JN695047; AFH35126.1; -; Genomic_DNA. DR AlphaFoldDB; H9ZGM0; -. DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW. DR GO; GO:0003677; F:DNA binding; IEA:InterPro. DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC. DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro. DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro. DR Gene3D; 2.40.50.100; -; 1. DR Gene3D; 2.40.50.150; -; 1. DR Gene3D; 3.90.1100.10; -; 1. DR Gene3D; 2.30.150.10; DNA-directed RNA polymerase, beta subunit, external 1 domain; 1. DR Gene3D; 2.40.270.10; DNA-directed RNA polymerase, subunit 2, domain 6; 1. DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf. DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1. DR InterPro; IPR015712; DNA-dir_RNA_pol_su2. DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom. DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf. DR InterPro; IPR007645; RNA_pol_Rpb2_3. DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold. DR PANTHER; PTHR20856; DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2; 1. DR PANTHER; PTHR20856:SF20; DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA; 1. DR Pfam; PF04565; RNA_pol_Rpb2_3; 1. DR Pfam; PF10385; RNA_pol_Rpb2_45; 1. DR Pfam; PF00562; RNA_pol_Rpb2_6; 1. DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1. PE 3: Inferred from homology; KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478, KW ECO:0000313|EMBL:AFH35126.1}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, KW ECO:0000313|EMBL:AFH35126.1}; KW Transcription {ECO:0000256|ARBA:ARBA00023163}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AFH35126.1}. FT DOMAIN 47..114 FT /note="RNA polymerase Rpb2" FT /evidence="ECO:0000259|Pfam:PF04565" FT DOMAIN 125..190 FT /note="DNA-directed RNA polymerase beta subunit external 1" FT /evidence="ECO:0000259|Pfam:PF10385" FT DOMAIN 251..394 FT /note="DNA-directed RNA polymerase subunit 2 hybrid- FT binding" FT /evidence="ECO:0000259|Pfam:PF00562" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:AFH35126.1" FT NON_TER 395 FT /evidence="ECO:0000313|EMBL:AFH35126.1" SQ SEQUENCE 395 AA; 43134 MW; D6F836201AF04736 CRC64; GLVRVERAVK ERLSMAESEG LMPQDLINAK PVAAAVKEFF GSSQLSQFMD QNNPLSEITH KRRVSALGPG GLTRERAGFE VRDVHPTHYG RVCPIETPEG PNIGLINSLA AYARTNQYGF LESPYRVVKE GVVTDDIVFL SAIEEADHVI AQASAAMSEK KQLIDELVAV RHLNEFTVKA PEDVTLMDVS PKQVVSVAAS LIPFLEHDDA NRALMGSNMQ RQAVPTLRAD KPLVGTGMER NVARDSGVCV VARRGGVIDS VDASRIVVRV ADDEVETGEA GVDIYNLTKY TRSNQNTCIN QRPLVSKGDV VQRSDIMADG PSTDMGELAL GQNMRIAFMA WNGFNFEDSI CLSERVVQED RFTTIHIQEL TCVARDTKLG PEEITADIPN VGEAA //