ID H2IS53_RAHAC Unreviewed; 504 AA. AC H2IS53; DT 21-MAR-2012, integrated into UniProtKB/TrEMBL. DT 21-MAR-2012, sequence version 1. DT 11-MAY-2016, entry version 27. DE RecName: Full=Bifunctional NAD(P)H-hydrate repair enzyme {ECO:0000256|PIRNR:PIRNR017184}; DE AltName: Full=Nicotinamide nucleotide repair protein {ECO:0000256|PIRNR:PIRNR017184}; GN OrderedLocusNames=Rahaq2_0450 {ECO:0000313|EMBL:AEX50384.1}; OS Rahnella aquatilis (strain ATCC 33071 / DSM 4594 / JCM 1683 / NBRC OS 105701 / NCIMB 13365 / CIP 78.65). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Rahnella. OX NCBI_TaxID=745277 {ECO:0000313|EMBL:AEX50384.1, ECO:0000313|Proteomes:UP000009010}; RN [1] {ECO:0000313|Proteomes:UP000009010} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 33071 / DSM 4594 / JCM 1683 / NBRC 105701 / NCIMB 13365 / RC CIP 78.65 {ECO:0000313|Proteomes:UP000009010}; RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., RA Peters L., Ovchinnikova G., Held B., Detter J.C., Han C., Tapia R., RA Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Sobecky P., RA Martinez R., Woyke T.; RT "Complete sequence of chromosome of Rahnella aquatilis CIP 78.65."; RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Bifunctional enzyme that catalyzes the epimerization of CC the S- and R-forms of NAD(P)HX and the dehydration of the S-form CC of NAD(P)HX at the expense of ADP, which is converted to AMP. This CC allows the repair of both epimers of NAD(P)HX, a damaged form of CC NAD(P)H that is a result of enzymatic or heat-dependent hydration. CC {ECO:0000256|PIRNR:PIRNR017184}. CC -!- COFACTOR: CC Name=K(+); Xref=ChEBI:CHEBI:29103; CC Evidence={ECO:0000256|PIRNR:PIRNR017184}; CC Note=Binds 1 potassium ion per subunit. CC {ECO:0000256|PIRNR:PIRNR017184}; CC -!- SIMILARITY: In the C-terminal section; belongs to the NnrD/CARKD CC family. {ECO:0000256|PIRNR:PIRNR017184}. CC -!- SIMILARITY: In the N-terminal section; belongs to the NnrE/AIBP CC family. {ECO:0000256|PIRNR:PIRNR017184}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP003244; AEX50384.1; -; Genomic_DNA. DR RefSeq; WP_014333655.1; NZ_JMPO01000135.1. DR STRING; 745277.Rahaq2_0450; -. DR EnsemblBacteria; AEX50384; AEX50384; Rahaq2_0450. DR KEGG; raq:Rahaq2_0450; -. DR eggNOG; ENOG4105DR1; Bacteria. DR eggNOG; COG0062; LUCA. DR eggNOG; COG0063; LUCA. DR KO; K17758; -. DR KO; K17759; -. DR OMA; KGDHGRV; -. DR Proteomes; UP000009010; Chromosome. DR GO; GO:0052855; F:ADP-dependent NAD(P)H-hydrate dehydratase activity; IEA:InterPro. DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW. DR Gene3D; 3.40.1190.20; -; 1. DR Gene3D; 3.40.50.10260; -; 1. DR HAMAP; MF_01965; NADHX_dehydratase; 1. DR HAMAP; MF_01966; NADHX_epimerase; 1. DR InterPro; IPR017953; Carbohydrate_kinase_pred_CS. DR InterPro; IPR000631; CARKD. DR InterPro; IPR030677; Nnr. DR InterPro; IPR029056; Ribokinase-like. DR InterPro; IPR004443; YjeF_N_dom. DR Pfam; PF01256; Carb_kinase; 1. DR Pfam; PF03853; YjeF_N; 1. DR PIRSF; PIRSF017184; Nnr; 1. DR SUPFAM; SSF53613; SSF53613; 1. DR SUPFAM; SSF64153; SSF64153; 1. DR TIGRFAMs; TIGR00196; yjeF_cterm; 1. DR TIGRFAMs; TIGR00197; yjeF_nterm; 1. DR PROSITE; PS01050; YJEF_C_2; 1. DR PROSITE; PS51383; YJEF_C_3; 1. DR PROSITE; PS51385; YJEF_N; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|PIRNR:PIRNR017184}; KW Complete proteome {ECO:0000313|Proteomes:UP000009010}; KW Isomerase {ECO:0000256|PIRNR:PIRNR017184}; KW Kinase {ECO:0000313|EMBL:AEX50384.1}; KW Lyase {ECO:0000256|PIRNR:PIRNR017184}; KW Metal-binding {ECO:0000256|PIRNR:PIRNR017184}; KW NAD {ECO:0000256|PIRNR:PIRNR017184}; KW NADP {ECO:0000256|PIRNR:PIRNR017184}; KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR017184}; KW Potassium {ECO:0000256|PIRNR:PIRNR017184}; KW Reference proteome {ECO:0000313|Proteomes:UP000009010}; KW Transferase {ECO:0000313|EMBL:AEX50384.1}. FT DOMAIN 23 225 YjeF N-terminal. {ECO:0000259|PROSITE: FT PS51385}. SQ SEQUENCE 504 AA; 53247 MW; 45E3B22A5B00173A CRC64; MTNQREKRNE ASLPHSVFSA DWLRQAERQA AQETGISLFT LMQRAAKAAF SLAQREFPQM RHWLILCGHG NNGGDGFEVA RLAKNAGIRV TLLAVAGSKP LPEEAEAARE GWLKSGGTVN PADSAWPQDA DLIIDGLLGT GLSSAPRAPY DGLIDAINSA SLPVISLDIP SGLDAQTGQT AGKAVHATHT LSFIALKPGL LTGQARDYTG KLHCDALGLE SWLGGHLAPL QRLDATQLGQ WLHPRRPCSH KGEHGRLLVI GGDTGFAGAI RMASEAALRS GAGLVRVLTH KEHAGPLLTA RPELMVQELT AESLDESLEW ADVLVIGPGL GQKEWGKQAI ERVAKWDKPA LWDADALNLL AINPRKRQNR VLTPHPGEAA RLLGCSVKDI ESDRLLSARN LTQRYGGVVV LKGAGTLIAS ERGEMAVADV GNAGMASGGM GDVLSGIIGG LLAQKLNIYD AACAGSVVHG AAADEIAHRQ GTRGMLATDL LAVIAPLVNP DLKK //