ID H0JN08_9NOCA Unreviewed; 369 AA. AC H0JN08; DT 22-FEB-2012, integrated into UniProtKB/TrEMBL. DT 22-FEB-2012, sequence version 1. DT 16-MAY-2012, entry version 4. DE RecName: Full=Ribosomal RNA large subunit methyltransferase N; DE EC=2.1.1.192; DE AltName: Full=23S rRNA (adenine(2503)-C(2))-methyltransferase; DE AltName: Full=23S rRNA m2A2503 methyltransferase; GN Name=rlmN; ORFNames=AK37_04927; OS Rhodococcus pyridinivorans AK37. OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Nocardiaceae; Rhodococcus. OX NCBI_TaxID=1114960; RN [1] RP NUCLEOTIDE SEQUENCE. RC STRAIN=AK37; RA Kriszt B., Tancsics A., Cserhati M., Toth A., Nagy I., Horvath B., RA Tamura T., Kukolya J., Szoboszlay S.; RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Specifically methylates position 2 of adenine 2503 in CC 23S rRNA (By similarity). CC -!- CATALYTIC ACTIVITY: 2 S-adenosyl-L-methionine + adenine(2503) in CC 23S rRNA = S-adenosyl-L-homocysteine + L-methionine + 5'- CC deoxyadenosine + 2-methyladenine(2503) in 23S rRNA. CC -!- COFACTOR: Binds 1 4Fe-4S cluster. The cluster is coordinated with CC 3 cysteines and an exchangeable S-adenosyl-L-methionine (By CC similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the radical SAM superfamily. RlmN family. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AHBW01000031; EHK85269.1; -; Genomic_DNA. DR GO; GO:0005737; C:cytoplasm; IEA:HAMAP. DR GO; GO:0005506; F:iron ion binding; IEA:HAMAP. DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro. DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro. DR GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IEA:HAMAP. DR GO; GO:0006364; P:rRNA processing; IEA:HAMAP. DR Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1. DR HAMAP; MF_01849; 23SrRNA_methyltr_N; 1; -. DR InterPro; IPR013785; Aldolase_TIM. DR InterPro; IPR006638; Elp3/MiaB/NifB. DR InterPro; IPR004383; rRNA_lsu_MTrfase_RlmN. DR InterPro; IPR007197; rSAM. DR Pfam; PF04055; Radical_SAM; 1. DR PIRSF; PIRSF006004; CHP00048; 1. DR SMART; SM00729; Elp3; 1. DR TIGRFAMs; TIGR00048; TIGR00048; 1. PE 3: Inferred from homology; KW 4Fe-4S; Cytoplasm; Iron; Iron-sulfur; Metal-binding; KW Methyltransferase; rRNA processing; S-adenosyl-L-methionine; KW Transferase. FT METAL 128 128 Iron-sulfur (4Fe-4S-S-AdoMet) (By FT similarity). FT METAL 132 132 Iron-sulfur (4Fe-4S-S-AdoMet) (By FT similarity). FT METAL 135 135 Iron-sulfur (4Fe-4S-S-AdoMet) (By FT similarity). SQ SEQUENCE 369 AA; 39754 MW; BC12E39692529318 CRC64; MASSLPLVFD APKRGLPPRH LADLDADQRR DAVRELGLPA FRADQLARQY YGRLEADPAK MTDLPAAVRD KVGESLFPPL LSAVKHIACD GGETRKTLWR AHDGTLLESV LMRYPDRATL CISSQAGCGM ACPFCATGQG GLDRNLSTAE IVDQVRAAAA ALRDGDVAGG PGRLSNVVFM GMGEPLANYK RVVAAVRRIT SPAPEGLGLS QRSVTVSTVG LAPAIRRLAD EGLSVTLAVS LHTPDDELRD TLVPVNNRWP VAEVLEAARY YADKTGRRVS IEYALIRDVN DQPWRADMLG KKLHKALGSL VHVNLIPLNP TPGSEWDASP KDVEREFVRR VQAQGVSCTV RDTRGQEIAA ACGQLAAEG //