ID G0SH86_CHATD Unreviewed; 310 AA. AC G0SH86; DT 19-OCT-2011, integrated into UniProtKB/TrEMBL. DT 19-OCT-2011, sequence version 1. DT 07-SEP-2016, entry version 18. DE RecName: Full=Cytosolic Fe-S cluster assembly factor CFD1 {ECO:0000256|HAMAP-Rule:MF_03039}; DE AltName: Full=Cytosolic Fe-S cluster-deficient protein 1 {ECO:0000256|HAMAP-Rule:MF_03039}; GN Name=CFD1 {ECO:0000256|HAMAP-Rule:MF_03039}; GN ORFNames=CTHT_0069100 {ECO:0000313|EMBL:EGS17575.1}; OS Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; OC Sordariomycetes; Sordariomycetidae; Sordariales; Chaetomiaceae; OC Chaetomium. OX NCBI_TaxID=759272 {ECO:0000313|Proteomes:UP000008066}; RN [1] {ECO:0000313|EMBL:EGS17575.1, ECO:0000313|Proteomes:UP000008066} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DSM 1495 / CBS 144.50 / IMI 039719 RC {ECO:0000313|Proteomes:UP000008066}; RX PubMed=21784248; DOI=10.1016/j.cell.2011.06.039; RA Amlacher S., Sarges P., Flemming D., van Noort V., Kunze R., RA Devos D.P., Arumugam M., Bork P., Hurt E.; RT "Insight into structure and assembly of the nuclear pore complex by RT utilizing the genome of a eukaryotic thermophile."; RL Cell 146:277-289(2011). CC -!- FUNCTION: Component of the cytosolic iron-sulfur (Fe/S) protein CC assembly (CIA) machinery. Required for maturation of CC extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer CC forms a Fe-S scaffold complex, mediating the de novo assembly of CC an Fe-S cluster and its transfer to target apoproteins. CC {ECO:0000256|HAMAP-Rule:MF_03039}. CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Evidence={ECO:0000256|HAMAP-Rule:MF_03039}; CC Note=Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two CC stable clusters in the N-termini of NBP35 and two labile, bridging CC clusters between subunits of the NBP35-CFD1 heterotetramer. CC {ECO:0000256|HAMAP-Rule:MF_03039}; CC -!- SUBUNIT: Heterotetramer of 2 NBP35 and 2 CFD1 chains. CC {ECO:0000256|HAMAP-Rule:MF_03039}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03039}. CC -!- SIMILARITY: Belongs to the Mrp/NBP35 ATP-binding proteins family. CC NUBP2/CFD1 subfamily. {ECO:0000256|HAMAP-Rule:MF_03039}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; GL988047; EGS17575.1; -; Genomic_DNA. DR RefSeq; XP_006697193.1; XM_006697130.1. DR EnsemblFungi; EGS17575; EGS17575; CTHT_0069100. DR GeneID; 18260948; -. DR KEGG; cthr:CTHT_0069100; -. DR OrthoDB; EOG092C4BLN; -. DR Proteomes; UP000008066; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-HAMAP. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:UniProtKB-HAMAP. DR Gene3D; 3.40.50.300; -; 1. DR HAMAP; MF_02040; Mrp_NBP35; 1. DR HAMAP; MF_03039; NUBP2; 1. DR InterPro; IPR019591; Mrp/NBP35_ATP-bd. DR InterPro; IPR000808; Mrp_CS. DR InterPro; IPR028600; NUBP2/Cfd1_eukaryotes. DR InterPro; IPR027417; P-loop_NTPase. DR PANTHER; PTHR23264; PTHR23264; 2. DR Pfam; PF10609; ParA; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR PROSITE; PS01215; MRP; 1. PE 3: Inferred from homology; KW 4Fe-4S {ECO:0000256|HAMAP-Rule:MF_03039}; KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_03039}; KW Complete proteome {ECO:0000313|Proteomes:UP000008066}; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03039}; KW Iron {ECO:0000256|HAMAP-Rule:MF_03039}; KW Iron-sulfur {ECO:0000256|HAMAP-Rule:MF_03039}; KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_03039}; KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_03039}; KW Reference proteome {ECO:0000313|Proteomes:UP000008066}. FT NP_BIND 15 22 ATP. {ECO:0000256|HAMAP-Rule:MF_03039}. FT METAL 199 199 Iron-sulfur (4Fe-4S); shared with dimeric FT partner. {ECO:0000256|HAMAP-Rule: FT MF_03039}. FT METAL 202 202 Iron-sulfur (4Fe-4S); shared with dimeric FT partner. {ECO:0000256|HAMAP-Rule: FT MF_03039}. SQ SEQUENCE 310 AA; 33239 MW; 0F3BE634AEB4BD6E CRC64; MSLSQVKHII LVLSGKGGVG KSSVTTQLAL SLSQAGYSVG VLDVDLTGPS IPRMFAVEDA KVKQGSGGWL PVVVHEANPS TGIGSLRVMS LGFLLPRRGD AVIWRGPKKT AMVRQFMSDV LWDELDFLLV DTPPGTSDEH ISLAETLLQE ARPGQLSGAI VVTTPQAVAT ADVRKELNFC KKTGIRVLGV VENMSGFVCP NCSECTNIFS SGGGEIMAND FNVRFLGRVP IDPQFLVLIE TGKRPRYPEG TKVNSQDLSF QHLEQVDNAD NPETPNSSLL VDKYRDCSLA PIFRAITADV VVAVEQASGS //