ID F8JX84_STREN Unreviewed; 909 AA. AC F8JX84; G8X2B4; DT 21-SEP-2011, integrated into UniProtKB/TrEMBL. DT 21-SEP-2011, sequence version 1. DT 28-JUN-2023, entry version 63. DE SubName: Full=Putative penicillin acylase {ECO:0000313|EMBL:AEW94549.1}; GN OrderedLocusNames=SCATT_21780 {ECO:0000313|EMBL:AEW94549.1}; OS Streptantibioticus cattleyicolor (strain ATCC 35852 / DSM 46488 / JCM 4925 OS / NBRC 14057 / NRRL 8057) (Streptomyces cattleya). OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales; OC Streptomycetaceae; Streptantibioticus. OX NCBI_TaxID=1003195 {ECO:0000313|EMBL:AEW94549.1, ECO:0000313|Proteomes:UP000007842}; RN [1] {ECO:0000313|Proteomes:UP000007842} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 35852 / DSM 46488 / JCM 4925 / NBRC 14057 / NRRL 8057 RC {ECO:0000313|Proteomes:UP000007842}; RA Ou H.-Y., Li P., Zhao C., O'Hagan D., Deng Z.; RT "Complete genome sequence of Streptomyces cattleya strain DSM 46488."; RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases. CC -!- COFACTOR: CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; CC Evidence={ECO:0000256|PIRSR:PIRSR001227-2}; CC Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2}; CC -!- SIMILARITY: Belongs to the peptidase S45 family. CC {ECO:0000256|ARBA:ARBA00006586}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP003219; AEW94549.1; -; Genomic_DNA. DR RefSeq; WP_014142944.1; NC_017586.1. DR AlphaFoldDB; F8JX84; -. DR MEROPS; S45.003; -. DR EnsemblBacteria; AEW94549; AEW94549; SCATT_21780. DR KEGG; scy:SCATT_21780; -. DR PATRIC; fig|1003195.11.peg.3711; -. DR eggNOG; COG2366; Bacteria. DR HOGENOM; CLU_011790_0_1_11; -. DR OMA; EMDVRRH; -. DR OrthoDB; 9759796at2; -. DR Proteomes; UP000007842; Chromosome. DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro. DR CDD; cd03747; Ntn_PGA_like; 1. DR Gene3D; 2.30.120.10; -; 1. DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 2. DR Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1. DR InterPro; IPR029055; Ntn_hydrolases_N. DR InterPro; IPR014395; Pen/GL7ACA/AHL_acylase. DR InterPro; IPR023343; Penicillin_amidase_dom1. DR InterPro; IPR043146; Penicillin_amidase_N_B-knob. DR InterPro; IPR002692; S45. DR PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1. DR PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1. DR Pfam; PF01804; Penicil_amidase; 1. DR PIRSF; PIRSF001227; Pen_acylase; 1. DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1. PE 3: Inferred from homology; KW Calcium {ECO:0000256|PIRSR:PIRSR001227-2}; KW Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2}; KW Reference proteome {ECO:0000313|Proteomes:UP000007842}. FT ACT_SITE 299 FT /note="Nucleophile" FT /evidence="ECO:0000256|PIRSR:PIRSR001227-1" FT BINDING 210 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2" FT BINDING 377 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2" FT BINDING 380 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2" SQ SEQUENCE 909 AA; 99746 MW; C2D589FC334DCE4C CRC64; MPANPSGPPA KKKGRRARLV VILVVLLLVA GIGYGSYWSV STVRASFPVT TGTLKLNGLT APVQVKRDDH GIPQIYADDA ADLFRAQGYV QAQDRFWEMD VRRHLTAGRL SEMFGSGQVE NDAFIRTLGW RRVAQQEWDT QLSPTAKAYL RAYSDGVNAY LSGHHGSALS VEYAVLGLTH SYTPERWTPV DSISWLKAMA WDLRSNIKDE IDRALMTSRL TPAQIAQLYP EYPYGRNRPI TGGGSVTPDG SEYVPAGRTA TGGGQTTSQR YPNAQLSALS RTIDQLPALL GPSGNGIGSN SWVVSGTYTT TGKPMLANDP HLAPQMPSLW YQMGLHCRTV NAACPYDVSG YTFSGMPGVV IGHNQDIAWG MTNMGADVTD LYLEKVTADS YLYDGKQVPF RTRQETIKVA GGSSRVITVR ETRDGMPLIS DRSDEMAEVG QNAPVGQTAP DRRDGYAVAL RWTALTPGKT MEAVFELDRA TDFTHFRKAA QDFAVPAQNL VYADTRNNIG YQAPGMIPVR HKVTGGIPDD GRYPVPGWDP DYRWTGYIPA AALPWEYNPK RGYIVTANQA VTDKKYPYLL TDDWGYGTRS QRINDLIASK IAGGGKISMD DMQSMQMDDS SEIAKLLVPY LLKVDISDPY VRQAQQLLEG WDFNQDGDSA AAAYFNAVWR NVLKLAFGNK LPKEVRPKGQ CLRVPPAGGS GPVDDLGTPK LVTECGQRDP AEAQPDGGDR WFEVVRAILP DATNPWWNTS TTGAKNGTDR DQLLAKAMKD ARWELTSKLG KDINTWSWGR LHKMTLRNQT LGTQGPAIVK WLLNRGPWQL GGGEAAVNAT GWNAAAGYDV VWVPSMRMIV SLDDFDKSRW INLTGASGHA YDAHYSDQTA MWVDGQLLPW AYSGGAVDKA AQDKLTLTP //