ID   F4ZLQ3_THETY            Unreviewed;       250 AA.
AC   F4ZLQ3;
DT   28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT   28-JUN-2011, sequence version 1.
DT   13-SEP-2023, entry version 34.
DE   RecName: Full=DNA-directed RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00015972};
DE            EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418};
DE   AltName: Full=RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00033070};
DE   AltName: Full=Transcriptase subunit alpha {ECO:0000256|ARBA:ARBA00032524};
DE   Flags: Fragment;
GN   Name=rpoA {ECO:0000313|EMBL:AEB92268.1};
OS   Thermoanaerobacter thermohydrosulfuricus (Clostridium
OS   thermohydrosulfuricum).
OC   Bacteria; Bacillota; Clostridia; Thermoanaerobacterales;
OC   Thermoanaerobacteraceae; Thermoanaerobacter.
OX   NCBI_TaxID=1516 {ECO:0000313|EMBL:AEB92268.1};
RN   [1] {ECO:0000313|EMBL:AEB92268.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=WC12 {ECO:0000313|EMBL:AEB92268.1};
RX   PubMed=21392917; DOI=10.1016/j.syapm.2010.11.019;
RA   Verbeke T.J., Sparling R., Hill J.E., Links M.G., Levin D.,
RA   Dumonceaux T.J.;
RT   "Predicting relatedness of bacterial genomes using the chaperonin-60
RT   universal target (cpn60 UT): Application to Thermoanaerobacter species.";
RL   Syst. Appl. Microbiol. 34:171-179(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550};
CC   -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC       {ECO:0000256|ARBA:ARBA00007123}.
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DR   EMBL; HQ153796; AEB92268.1; -; Genomic_DNA.
DR   AlphaFoldDB; F4ZLQ3; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR   CDD; cd06928; RNAP_alpha_NTD; 1.
DR   Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR   Gene3D; 2.170.120.12; DNA-directed RNA polymerase, insert domain; 1.
DR   Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR   InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR   InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR   InterPro; IPR011773; DNA-dir_RpoA.
DR   InterPro; IPR036603; RBP11-like.
DR   InterPro; IPR011260; RNAP_asu_C.
DR   InterPro; IPR036643; RNApol_insert_sf.
DR   NCBIfam; TIGR02027; rpoA; 1.
DR   Pfam; PF01000; RNA_pol_A_bac; 1.
DR   Pfam; PF03118; RNA_pol_A_CTD; 1.
DR   Pfam; PF01193; RNA_pol_L; 1.
DR   SMART; SM00662; RPOLD; 1.
DR   SUPFAM; SSF47789; C-terminal domain of RNA polymerase alpha subunit; 1.
DR   SUPFAM; SSF56553; Insert subdomain of RNA polymerase alpha subunit; 1.
DR   SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW   ECO:0000313|EMBL:AEB92268.1};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW   Transcription {ECO:0000256|ARBA:ARBA00023163};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          1..191
FT                   /note="DNA-directed RNA polymerase RpoA/D/Rpb3-type"
FT                   /evidence="ECO:0000259|SMART:SM00662"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:AEB92268.1"
FT   NON_TER         250
FT                   /evidence="ECO:0000313|EMBL:AEB92268.1"
SQ   SEQUENCE   250 AA;  27788 MW;  BAC8FE7FA83C3F70 CRC64;
     GNSLRRVLLS SLPGAAPKSV KIDGVLHEFS TLPGVKEDVV KIILNLKEVA VKLHSDEPVV
     GRIDVEGKGE VTAGHIKGSA DIEILNPDHH IATLSDDGKL HMEIVFVKGK GYVPSDKNKE
     PNQPIGVIPV DSIFTPVKRV SYNVKNTRVG HVTDYDKLTL EVWTNGTITP KEAISMASDM
     LIKYFQKFIS FTGEYKSSDI FVDKPEKKVE KPLDMTIEEL DLFVRSYNCL KRAGINTVQE
     LTQKTEEEMM
//