ID NCER3_ARATH Reviewed; 733 AA. AC F4KHQ8; Q93ZI6; Q9FIL4; DT 10-MAY-2017, integrated into UniProtKB/Swiss-Prot. DT 28-JUN-2011, sequence version 1. DT 12-OCT-2022, entry version 69. DE RecName: Full=Neutral ceramidase 3 {ECO:0000303|PubMed:26150824}; DE Short=AtNCER3 {ECO:0000303|PubMed:26150824}; DE Short=N-CDase 3 {ECO:0000303|PubMed:26150824}; DE Short=NCDase 3 {ECO:0000303|PubMed:26150824}; DE EC=3.5.1.23 {ECO:0000250|UniProtKB:O06769}; DE AltName: Full=Acylsphingosine deacylase 3; DE AltName: Full=N-acylsphingosine amidohydrolase 3; DE Flags: Precursor; GN Name=NCER3 {ECO:0000303|PubMed:26150824}; GN OrderedLocusNames=At5g58980 {ECO:0000312|Araport:AT5G58980}; GN ORFNames=K19M22.17 {ECO:0000312|EMBL:BAB09641.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=10048488; DOI=10.1093/dnares/5.6.379; RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., RA Tabata S.; RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence RT features of the regions of 1,081,958 bp covered by seventeen physically RT assigned P1 and TAC clones."; RL DNA Res. 5:379-391(1998). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., RA Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [4] RP FUNCTION, DISRUPTION PHENOTYPE, GENE FAMILY, AND NOMENCLATURE. RC STRAIN=cv. Columbia; RX PubMed=26150824; DOI=10.3389/fpls.2015.00460; RA Li J., Bi F.-C., Yin J., Wu J.-X., Rong C., Wu J.-L., Yao N.; RT "An Arabidopsis neutral ceramidase mutant ncer1 accumulates RT hydroxyceramides and is sensitive to oxidative stress."; RL Front. Plant Sci. 6:460-460(2015). CC -!- FUNCTION: Hydrolyzes the sphingolipid ceramide into sphingosine and CC free fatty acid (By similarity). Promotes oxidative stress resistance CC (PubMed:26150824). {ECO:0000250|UniProtKB:F4HQM3, CC ECO:0000269|PubMed:26150824}. CC -!- CATALYTIC ACTIVITY: CC Reaction=an N-acylsphing-4-enine + H2O = a fatty acid + sphing-4-enine; CC Xref=Rhea:RHEA:20856, ChEBI:CHEBI:15377, ChEBI:CHEBI:28868, CC ChEBI:CHEBI:52639, ChEBI:CHEBI:57756; EC=3.5.1.23; CC Evidence={ECO:0000250|UniProtKB:O06769}; CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9VA70}. CC Endoplasmic reticulum {ECO:0000250|UniProtKB:F4HQM3}. Golgi apparatus CC {ECO:0000250|UniProtKB:Q0JL46}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=F4KHQ8-1; Sequence=Displayed; CC Name=2; CC IsoId=F4KHQ8-2; Sequence=VSP_058913; CC -!- DISRUPTION PHENOTYPE: Increased sensitivity to C2-ceramide induced cell CC death. {ECO:0000269|PubMed:26150824}. CC -!- SIMILARITY: Belongs to the neutral ceramidase family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=BAB09641.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB016885; BAB09641.1; ALT_SEQ; Genomic_DNA. DR EMBL; CP002688; AED97126.1; -; Genomic_DNA. DR EMBL; AY057506; AAL09747.1; -; mRNA. DR RefSeq; NP_200706.1; NM_125288.4. [F4KHQ8-1] DR AlphaFoldDB; F4KHQ8; -. DR SMR; F4KHQ8; -. DR STRING; 3702.AT5G58980.1; -. DR PaxDb; F4KHQ8; -. DR ProteomicsDB; 251206; -. [F4KHQ8-1] DR EnsemblPlants; AT5G58980.1; AT5G58980.1; AT5G58980. [F4KHQ8-1] DR GeneID; 836015; -. DR Gramene; AT5G58980.1; AT5G58980.1; AT5G58980. [F4KHQ8-1] DR KEGG; ath:AT5G58980; -. DR Araport; AT5G58980; -. DR TAIR; locus:2154598; AT5G58980. DR eggNOG; KOG2232; Eukaryota. DR HOGENOM; CLU_011300_2_0_1; -. DR InParanoid; F4KHQ8; -. DR OMA; DWPGAFD; -. DR OrthoDB; 967085at2759; -. DR PRO; PR:F4KHQ8; -. DR Proteomes; UP000006548; Chromosome 5. DR ExpressionAtlas; F4KHQ8; baseline and differential. DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell. DR GO; GO:0005576; C:extracellular region; IBA:GO_Central. DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell. DR GO; GO:0102121; F:ceramidase activity; IEA:UniProtKB-EC. DR GO; GO:0017040; F:N-acylsphingosine amidohydrolase activity; IBA:GO_Central. DR GO; GO:0034599; P:cellular response to oxidative stress; IMP:UniProtKB. DR GO; GO:0046514; P:ceramide catabolic process; IBA:GO_Central. DR GO; GO:0042759; P:long-chain fatty acid biosynthetic process; IBA:GO_Central. DR GO; GO:0046512; P:sphingosine biosynthetic process; IBA:GO_Central. DR Gene3D; 2.60.40.2300; -; 1. DR InterPro; IPR006823; Ceramidase_alk. DR InterPro; IPR038445; NCDase_C_sf. DR InterPro; IPR031331; NEUT/ALK_ceramidase_C. DR InterPro; IPR031329; NEUT/ALK_ceramidase_N. DR PANTHER; PTHR12670; PTHR12670; 2. DR Pfam; PF04734; Ceramidase_alk; 1. DR Pfam; PF17048; Ceramidse_alk_C; 1. PE 2: Evidence at transcript level; KW Alternative splicing; Endoplasmic reticulum; Glycoprotein; Golgi apparatus; KW Hydrolase; Lipid metabolism; Reference proteome; Secreted; Signal; KW Sphingolipid metabolism. FT SIGNAL 1..25 FT /evidence="ECO:0000255" FT CHAIN 26..733 FT /note="Neutral ceramidase 3" FT /id="PRO_5003311651" FT ACT_SITE 307 FT /note="Nucleophile" FT /evidence="ECO:0000250|UniProtKB:Q9NR71" FT CARBOHYD 325 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498" FT VAR_SEQ 1..432 FT /note="MTRWSMSMHCTLFLLFLLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYAN FT MEQVASGVHFRLRARAFIVAEPYKKRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTE FT ENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSIL FT INKGELLDAGVNRSPSAYLNNPAHERSKYEYDVDKEMTLVKFVDDQWGPVARIMEDWFE FT RENGCRSVDVESPRRVSSIISDPYDQDLMEMASSLLSTGGKTVTRMSSVARRVRSRFRH FT ADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEF FT ESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCP FT AAMGFGFAAGTTDGPGAFDFKQGDDQ -> MFSTILYVVTLCK (in isoform 2)" FT /id="VSP_058913" SQ SEQUENCE 733 AA; 81838 MW; 4E00C150E5FCEED9 CRC64; MTRWSMSMHC TLFLLFLLRL TCIFSDSDYL MGLGSYDITG PAADVNMMGY ANMEQVASGV HFRLRARAFI VAEPYKKRIA FVNLDAGMAS QLVTIKVIER LKQRYGELYT EENVAISGTH THAGPGGYLQ YILYLVTSLG FVHQSFNALV DGIEQSIIQA HENLRPGSIL INKGELLDAG VNRSPSAYLN NPAHERSKYE YDVDKEMTLV KFVDDQWGPV ARIMEDWFER ENGCRSVDVE SPRRVSSIIS DPYDQDLMEM ASSLLSTGGK TVTRMSSVAR RVRSRFRHAD KPRFVSAFCQ TNCGDVSPNV LGAFCIDTGL PCEFNQSTCG GKNEQCYGRG PGYPDEFEST RIIGERQFKK AADLFTKASE EIQGKVDYRH AYVDFSQLEV TINGQNGGSE VVKTCPAAMG FGFAAGTTDG PGAFDFKQGD DQGNPFWRLV RNLLKNPTEE QVRCQRPKPI LLDTGEMKQP YDWAPSILPV QILRIGQLVI LCVPGEFTTM AGRRLRDAVK TVLKEGSNGR EFSVVIAGLT NSYSQYIATF EEYQVQRYEG ASTLYGPHTL SGYIQEFKKL ANDLLSAQTT DPGPQPPDLL HKQISLLTPV VADMTPIGTA FGDVTSDVPR LSKFRKGADI VRVQFRSANP RNDLMTEGTF ALVERWLEGR ETWVPVYDDD DFCLRFKWSR PFKLSTQSTA TIEWRIPETA SPGVYRITHF GSAKTPISSI HHFSGSSSAF VVY //