ID   E9Q3V0_MOUSE            Unreviewed;       637 AA.
AC   E9Q3V0;
DT   05-APR-2011, integrated into UniProtKB/TrEMBL.
DT   05-APR-2011, sequence version 1.
DT   22-FEB-2023, entry version 72.
DE   RecName: Full=Sodium- and chloride-dependent glycine transporter 1 {ECO:0000256|ARBA:ARBA00022312};
DE   AltName: Full=Solute carrier family 6 member 9 {ECO:0000256|ARBA:ARBA00031645};
GN   Name=Slc6a9 {ECO:0000313|Ensembl:ENSMUSP00000127289.2,
GN   ECO:0000313|MGI:MGI:95760};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000127289.2, ECO:0000313|Proteomes:UP000000589};
RN   [1] {ECO:0000313|Ensembl:ENSMUSP00000127289.2, ECO:0000313|Proteomes:UP000000589}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000127289.2,
RC   ECO:0000313|Proteomes:UP000000589};
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2] {ECO:0007829|PubMed:21183079}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [3] {ECO:0000313|Ensembl:ENSMUSP00000127289.2}
RP   IDENTIFICATION.
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000127289.2};
RG   Ensembl;
RL   Submitted (OCT-2022) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=chloride(out) + glycine(out) + 2 Na(+)(out) = chloride(in) +
CC         glycine(in) + 2 Na(+)(in); Xref=Rhea:RHEA:70691, ChEBI:CHEBI:17996,
CC         ChEBI:CHEBI:29101, ChEBI:CHEBI:57305;
CC         Evidence={ECO:0000256|ARBA:ARBA00033997};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC       Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC       2.A.22) family. SLC6A9 subfamily. {ECO:0000256|ARBA:ARBA00010056}.
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DR   AlphaFoldDB; E9Q3V0; -.
DR   SMR; E9Q3V0; -.
DR   MaxQB; E9Q3V0; -.
DR   PeptideAtlas; E9Q3V0; -.
DR   ProteomicsDB; 357530; -.
DR   Antibodypedia; 2813; 75 antibodies from 13 providers.
DR   Ensembl; ENSMUST00000163288.2; ENSMUSP00000127289.2; ENSMUSG00000028542.18.
DR   AGR; MGI:95760; -.
DR   MGI; MGI:95760; Slc6a9.
DR   VEuPathDB; HostDB:ENSMUSG00000028542; -.
DR   GeneTree; ENSGT00940000157263; -.
DR   HOGENOM; CLU_006855_9_4_1; -.
DR   PhylomeDB; E9Q3V0; -.
DR   ChiTaRS; Slc6a9; mouse.
DR   Proteomes; UP000000589; Chromosome 4.
DR   Bgee; ENSMUSG00000028542; Expressed in retinal neural layer and 237 other tissues.
DR   ExpressionAtlas; E9Q3V0; baseline and differential.
DR   GO; GO:0016324; C:apical plasma membrane; IEA:Ensembl.
DR   GO; GO:0016323; C:basolateral plasma membrane; IEA:Ensembl.
DR   GO; GO:0031045; C:dense core granule; IEA:Ensembl.
DR   GO; GO:0005768; C:endosome; IEA:Ensembl.
DR   GO; GO:0098686; C:hippocampal mossy fiber to CA3 synapse; IEA:Ensembl.
DR   GO; GO:0016328; C:lateral plasma membrane; IEA:Ensembl.
DR   GO; GO:0098688; C:parallel fiber to Purkinje cell synapse; IEA:Ensembl.
DR   GO; GO:0014069; C:postsynaptic density; IEA:Ensembl.
DR   GO; GO:0045211; C:postsynaptic membrane; IEA:Ensembl.
DR   GO; GO:0042734; C:presynaptic membrane; IEA:Ensembl.
DR   GO; GO:0030672; C:synaptic vesicle membrane; IEA:Ensembl.
DR   GO; GO:0015375; F:glycine:sodium symporter activity; IEA:Ensembl.
DR   GO; GO:1903804; P:glycine import across plasma membrane; IEA:Ensembl.
DR   GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR   InterPro; IPR000175; Na/ntran_symport.
DR   InterPro; IPR003028; Na/ntran_symport_glycine_GLY1.
DR   InterPro; IPR037272; SNS_sf.
DR   PANTHER; PTHR11616:SF263; SODIUM- AND CHLORIDE-DEPENDENT GLYCINE TRANSPORTER 1; 1.
DR   PANTHER; PTHR11616; SODIUM/CHLORIDE DEPENDENT TRANSPORTER; 1.
DR   Pfam; PF00209; SNF; 1.
DR   PRINTS; PR01204; GLY1TRNSPORT.
DR   PRINTS; PR00176; NANEUSMPORT.
DR   SUPFAM; SSF161070; SNF-like; 1.
DR   PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR   PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE   1: Evidence at protein level;
KW   Amino-acid transport {ECO:0000256|ARBA:ARBA00022970};
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Neurotransmitter transport {ECO:0000256|ARBA:ARBA00022775};
KW   Proteomics identification {ECO:0007829|EPD:E9Q3V0,
KW   ECO:0007829|MaxQB:E9Q3V0};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000589};
KW   Symport {ECO:0000256|ARBA:ARBA00022847};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT   TRANSMEM        60..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        114..139
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        219..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        252..279
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        299..316
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        328..353
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        387..408
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        429..455
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        461..481
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        501..524
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        536..561
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   REGION          1..44
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        22..44
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   637 AA;  70575 MW;  3A546991BF622B84 CRC64;
     MIGGDTRAAS AHPGMASAQG PVATPSPEQN GAVPSEATKK DQNLTRGNWG NQIEFVLTSV
     GYAVGLGAFM FPYFIMLIFC GIPLFFMELS FGQFASQGCL GVWRISPMFK GVGYGMMVVS
     TYIGIYYNVV ICIAFYYFFS SMTHVLPWAY CNNPWNTPDC AGVLDASNLT NGSRPAALSG
     NLSHLFNYTL QRTSPSEEYW RLYVLKLSDD IGNFGEVRLP LLGCLGVSWV VVFLCLIRGV
     KSSGKVVYFT ATFPYVVLTI LFVRGVTLEG AFTGIMYYLT PQWDKILEAK VWGDAASQIF
     YSLGCAWGGL ITMASYNKFH NNCYRDSVII SITNCATSVY AGFVIFSILG FMANHLGVDV
     SRVADHGPGL AFVAYPEALT LLPISPLWSL LFFFMLILLG LGTQFCLLET LVTAIVDEVG
     NEWILQKKTY VTLGVAVAGF LLGIPLTSQA GIYWLLLMDN YAASFSLVVI SCIMCVSIMY
     IYGHRNYFQD IQMMLGFPPP LFFQICWRFV SPAIIFFILI FTVIQYRPIT YNHYQYPGWA
     VAIGFLMALS SVICIPLYAL FQLCRTDGDT LLQRLKNATK PSRDWGPALL EHRTGRYAPT
     TTPSPEDGFE VQPLHPDKAQ IPIVGSNGSS RFQDSRI
//