ID E9KMS1_9INFA Unreviewed; 759 AA. AC E9KMS1; DT 05-APR-2011, integrated into UniProtKB/TrEMBL. DT 05-APR-2011, sequence version 1. DT 28-JUN-2023, entry version 54. DE RecName: Full=Polymerase basic protein 2 {ECO:0000256|ARBA:ARBA00014261, ECO:0000256|HAMAP-Rule:MF_04062}; DE AltName: Full=RNA-directed RNA polymerase subunit P3 {ECO:0000256|HAMAP-Rule:MF_04062}; GN Name=PB2 {ECO:0000256|HAMAP-Rule:MF_04062, GN ECO:0000256|RuleBase:RU361246, ECO:0000313|EMBL:ADD21402.1}; OS Influenza A virus (A/Hong Kong/415742/2009(H1N1)). OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus. OX NCBI_TaxID=735355 {ECO:0000313|EMBL:ADD21402.1, ECO:0000313|Proteomes:UP000132456}; RN [1] {ECO:0000313|EMBL:ADD21402.1, ECO:0000313|Proteomes:UP000132456} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=A/Hong Kong/415742/2009 {ECO:0000313|EMBL:ADD21402.1}; RX PubMed=20660098; DOI=10.1258/ebm.2010.010071; RA Zheng B., Chan K.H., Zhang A.J., Zhou J., Chan C.C., Poon V.K., Zhang K., RA Leung V.H., Jin D.Y., Woo P.C., Chan J.F., To K.K., Chen H., Yuen K.Y.; RT "D225G mutation in hemagglutinin of pandemic influenza H1N1 (2009) virus RT enhances virulence in mice."; RL Exp. Biol. Med. (Maywood) 235:981-988(2010). CC -!- FUNCTION: Plays an essential role in transcription initiation and cap- CC stealing mechanism, in which cellular capped pre-mRNAs are used to CC generate primers for viral transcription. Recognizes and binds the 7- CC methylguanosine-containing cap of the target pre-RNA which is CC subsequently cleaved after 10-13 nucleotides by the viral protein PA. CC Plays a role in the initiation of the viral genome replication and CC modulates the activity of the ribonucleoprotein (RNP) complex. CC {ECO:0000256|HAMAP-Rule:MF_04062}. CC -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1, CC PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). CC Interacts with nucleoprotein NP (via N-terminus). {ECO:0000256|HAMAP- CC Rule:MF_04062}. CC -!- SUBCELLULAR LOCATION: Host mitochondrion CC {ECO:0000256|ARBA:ARBA00004181}. Mitochondrion CC {ECO:0000256|ARBA:ARBA00004173}. Virion {ECO:0000256|HAMAP- CC Rule:MF_04062, ECO:0000256|RuleBase:RU361246}. Host nucleus CC {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|RuleBase:RU361246}. CC -!- SIMILARITY: Belongs to the influenza viruses PB2 family. CC {ECO:0000256|ARBA:ARBA00008660, ECO:0000256|HAMAP-Rule:MF_04062, CC ECO:0000256|RuleBase:RU361246}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_04062}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; GU931811; ADD21402.1; -; Viral_cRNA. DR Proteomes; UP000132456; Genome. DR GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell. DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-UniRule. DR GO; GO:0075526; P:cap snatching; IEA:UniProtKB-UniRule. DR GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule. DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-UniRule. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR Gene3D; 3.30.30.90; Polymerase Basic Protein 2, C-terminal domain; 1. DR HAMAP; MF_04062; INV_PB2; 1. DR InterPro; IPR001591; INV_PB2. DR InterPro; IPR037258; PDB2_C. DR Pfam; PF00604; Flu_PB2; 1. DR SUPFAM; SSF160453; PB2 C-terminal domain-like; 1. PE 3: Inferred from homology; KW Cap snatching {ECO:0000256|ARBA:ARBA00022715, ECO:0000256|HAMAP- KW Rule:MF_04062}; KW Eukaryotic host gene expression shutoff by virus {ECO:0000256|HAMAP- KW Rule:MF_04062}; KW Eukaryotic host transcription shutoff by virus KW {ECO:0000256|ARBA:ARBA00022731, ECO:0000256|HAMAP-Rule:MF_04062}; KW Host gene expression shutoff by virus {ECO:0000256|HAMAP-Rule:MF_04062}; KW Host nucleus {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|RuleBase:RU361246}; KW Host-virus interaction {ECO:0000256|HAMAP-Rule:MF_04062}; KW Inhibition of host RNA polymerase II by virus KW {ECO:0000256|ARBA:ARBA00023103, ECO:0000256|HAMAP-Rule:MF_04062}; KW mRNA capping {ECO:0000256|ARBA:ARBA00023042, ECO:0000256|HAMAP- KW Rule:MF_04062}; KW mRNA processing {ECO:0000256|ARBA:ARBA00022664, ECO:0000256|HAMAP- KW Rule:MF_04062}; KW Viral transcription {ECO:0000256|ARBA:ARBA00023314, ECO:0000256|HAMAP- KW Rule:MF_04062}; KW Virion {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|RuleBase:RU361246}. FT MOTIF 736..739 FT /note="Nuclear localization signal" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04062" FT SITE 627 FT /note="Avian adaptation" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04062" SQ SEQUENCE 759 AA; 85879 MW; 37964DF830D4BD7A CRC64; MERIKELRDL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM MAMRYPITAD KRIMDMIPER NEQGQTLWSK TNDAGSDRVM VSPLAVTWWN RNGSTTSTVH YPKVYKTYFE KVERLKHGTF GPVHFRNQVK IRRRVDTNPG HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLAITKEKK EELQDCKIAP LMVAYMLERE LVRKTRFLPV AGGTGSVYIE VLHLTQGTCW EQMYTPGGEV RNDDVDQSLI IAARNIVRRA AVSADPLASL LEMCHSTQIG GVRMVDILRQ NPTEEQAVDI CKAAIGLRIS SSFSFGGFTF KRTSGSSVKK EEEVLTGNLQ TLKIRVHEGY EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG IESIDNVMGM IGILPDMTPS TEMSLRGIRV SKMGVDEYSS TERVVVSIDR FLRVRDQRGN VLLSPEEVSE TQGTERLTIT YSSSMMWEIN GPESVLVNTY QWIIRNWEIV KIQWSQDPTM LYNKMEFEPF QSLVPKATRS RYSGFVRTLF QQMRDVLGTF DTVQIIKLLP FAAAPPEQSR MQFSSLTVNV RGSGLRILVR GNSPVFNYNK ATKRLTVLGK DAGALTEDPD EGTSGVESAV LRGFLILGKE DKRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRN SSILTDSQTA TKRIRMAIN //