ID E5KHU9_9ASTR Unreviewed; 173 AA. AC E5KHU9; DT 08-FEB-2011, integrated into UniProtKB/TrEMBL. DT 08-FEB-2011, sequence version 1. DT 22-FEB-2023, entry version 29. DE RecName: Full=Squalene synthase {ECO:0000256|RuleBase:RU368088}; DE EC=2.5.1.21 {ECO:0000256|RuleBase:RU368088}; DE Flags: Fragment; GN Name=SQS {ECO:0000313|EMBL:ADR66128.1}; OS Hieracium porrifolium. OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae; OC Hieraciinae; Hieracium. OX NCBI_TaxID=546202 {ECO:0000313|EMBL:ADR66128.1}; RN [1] {ECO:0000313|EMBL:ADR66128.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=22301891; DOI=10.3732/ajb.1100416; RA Krak K., Alvarez I., Caklova P., Costa A., Chrtek J., Fehrer J.; RT "Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) RT and their perspective for other tribes."; RL Am. J. Bot. 99:E74-E77(2012). CC -!- FUNCTION: Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) CC moieties to form squalene. {ECO:0000256|RuleBase:RU368088}. CC -!- CATALYTIC ACTIVITY: CC Reaction=2 (2E,6E)-farnesyl diphosphate + H(+) + NADH = 2 diphosphate + CC NAD(+) + squalene; Xref=Rhea:RHEA:32299, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:15440, ChEBI:CHEBI:33019, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:175763; EC=2.5.1.21; CC Evidence={ECO:0000256|ARBA:ARBA00001503}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32300; CC Evidence={ECO:0000256|ARBA:ARBA00001503}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2 (2E,6E)-farnesyl diphosphate + H(+) + NADPH = 2 diphosphate CC + NADP(+) + squalene; Xref=Rhea:RHEA:32295, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:15440, ChEBI:CHEBI:33019, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:175763; EC=2.5.1.21; CC Evidence={ECO:0000256|ARBA:ARBA00000041}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32296; CC Evidence={ECO:0000256|ARBA:ARBA00000041}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|ARBA:ARBA00001946, CC ECO:0000256|RuleBase:RU368088}; CC -!- COFACTOR: CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|ARBA:ARBA00001936}; CC -!- PATHWAY: Terpene metabolism; lanosterol biosynthesis; lanosterol from CC farnesyl diphosphate: step 1/3. {ECO:0000256|ARBA:ARBA00005057, CC ECO:0000256|RuleBase:RU368088}. CC -!- SIMILARITY: Belongs to the phytoene/squalene synthase family. CC {ECO:0000256|ARBA:ARBA00006251, ECO:0000256|RuleBase:RU368088}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; HQ131843; ADR66128.1; -; Genomic_DNA. DR AlphaFoldDB; E5KHU9; -. DR UniPathway; UPA00767; UER00751. DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW. DR GO; GO:0004310; F:farnesyl-diphosphate farnesyltransferase activity; IEA:UniProtKB-UniRule. DR GO; GO:0051996; F:squalene synthase activity; IEA:UniProtKB-UniRule. DR GO; GO:0008610; P:lipid biosynthetic process; IEA:InterPro. DR CDD; cd00683; Trans_IPPS_HH; 1. DR Gene3D; 1.10.600.10; Farnesyl Diphosphate Synthase; 1. DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf. DR InterPro; IPR002060; Squ/phyt_synthse. DR InterPro; IPR006449; Squal_synth-like. DR InterPro; IPR019845; Squalene/phytoene_synthase_CS. DR InterPro; IPR044844; Trans_IPPS_euk-type. DR InterPro; IPR033904; Trans_IPPS_HH. DR PANTHER; PTHR11626; FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE; 1. DR PANTHER; PTHR11626:SF2; SQUALENE SYNTHASE; 1. DR Pfam; PF00494; SQS_PSY; 1. DR SUPFAM; SSF48576; Terpenoid synthases; 1. DR TIGRFAMs; TIGR01559; squal_synth; 1. DR PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1. DR PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1. PE 3: Inferred from homology; KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU368088}. FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:ADR66128.1" FT NON_TER 173 FT /evidence="ECO:0000313|EMBL:ADR66128.1" SQ SEQUENCE 173 AA; 19862 MW; F6CA3ECD6AA0410D CRC64; HVSAAFLELK KGYQEAIEDI TMRMGAGMAK FICKEVETID DYDEYCHYVA GLVGLGLSKL FHAGGTEKLF PDSISNSMGL FLQKTNIIRD YLEDINEIPK SRMFWPREIW SKYVNKLEEL KYEENSEKAV QCLNDMVTNA LIHIEDCLKY MSELRDPAIF RFCAIPQIMA IGT //