ID KASSO_KASMA Reviewed; 65 AA. AC E4Z7G0; DT 03-APR-2013, integrated into UniProtKB/Swiss-Prot. DT 08-FEB-2011, sequence version 1. DT 01-MAY-2013, entry version 7. DE RecName: Full=Kassorin-M; DE AltName: Full=PreproKassorin-M; DE Flags: Precursor; OS Kassina maculata (African rhacophorid frog) (Hylambates maculatus). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Amphibia; Batrachia; Anura; Neobatrachia; Microhyloidea; Hyperoliidae; OC Kassina. OX NCBI_TaxID=8414; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AMIDATION RP AT LEU-64, AND MASS SPECTROMETRY. RC TISSUE=Skin; RX PubMed=21040978; DOI=10.1016/j.molimm.2010.09.018; RA Chen H., Wang L., Zeller M., Hornshaw M., Wu Y., Zhou M., Li J., RA Hang X., Cai J., Chen T., Shaw C.; RT "Kassorins: novel innate immune system peptides from skin secretions RT of the African hyperoliid frogs, Kassina maculata and Kassina RT senegalensis."; RL Mol. Immunol. 48:442-451(2011). CC -!- FUNCTION: Induces contraction of smooth muscle in isolated guinea CC pig urinary bladder (EC50=4.66 nM). Has no antimicrobial activity CC against the Gram-positive bacterium S.aureus, the Gram-negative CC bacterium E.coli and the yeast C.albicans. Elicits histamine CC release from rat peritoneal mast cells. CC -!- SUBCELLULAR LOCATION: Secreted. CC -!- TISSUE SPECIFICITY: Expressed by the skin glands. CC -!- MASS SPECTROMETRY: Mass=1388.82; Method=MALDI; Range=52-64; CC Note=With amidation; Source=PubMed:21040978; CC -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family. CC Brevinin subfamily. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; FN687445; CBK52153.1; -; mRNA. DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0042742; P:defense response to bacterium; IEA:InterPro. DR InterPro; IPR004275; Brevinin. DR Pfam; PF03032; Brevenin; 1. PE 1: Evidence at protein level; KW Amidation; Amphibian defense peptide; KW Cleavage on pair of basic residues; Secreted; Signal. FT SIGNAL 1 22 Potential. FT PROPEP 23 51 FT /FTId=PRO_0000421859. FT PEPTIDE 52 64 Kassorin-M. FT /FTId=PRO_5000666360. FT MOD_RES 64 64 Leucine amide. SQ SEQUENCE 65 AA; 7321 MW; B2756200A42F50CB CRC64; MLTLKKSMLL LFFLGMVSFS LADDKREDEA EEGEDKRADE GEEKRAAEKK RFLEGLLNTV TGLLG //