ID   KASSO_KASMA             Reviewed;          65 AA.
AC   E4Z7G0;
DT   03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 1.
DT   03-APR-2013, entry version 6.
DE   RecName: Full=Kassorin-M;
DE   AltName: Full=PreproKassorin-M;
DE   Flags: Precursor;
OS   Kassina maculata (African rhacophorid frog) (Hylambates maculatus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Amphibia; Batrachia; Anura; Neobatrachia; Microhyloidea; Hyperoliidae;
OC   Kassina.
OX   NCBI_TaxID=8414;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AMIDATION
RP   AT LEU-64, AND MASS SPECTROMETRY.
RC   TISSUE=Skin;
RX   PubMed=21040978; DOI=10.1016/j.molimm.2010.09.018;
RA   Chen H., Wang L., Zeller M., Hornshaw M., Wu Y., Zhou M., Li J.,
RA   Hang X., Cai J., Chen T., Shaw C.;
RT   "Kassorins: novel innate immune system peptides from skin secretions
RT   of the African hyperoliid frogs, Kassina maculata and Kassina
RT   senegalensis.";
RL   Mol. Immunol. 48:442-451(2011).
CC   -!- FUNCTION: Induces contraction of smooth muscle in isolated guinea
CC       pig urinary bladder (EC50=4.66 nM). Has no antimicrobial activity
CC       against the Gram-positive bacterium S.aureus, the Gram-negative
CC       bacterium E.coli and the yeast C.albicans. Elicits histamine
CC       release from rat peritoneal mast cells.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC   -!- MASS SPECTROMETRY: Mass=1388.82; Method=MALDI; Range=52-64;
CC       Note=With amidation; Source=PubMed:21040978;
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily.
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DR   EMBL; FN687445; CBK52153.1; -; mRNA.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:InterPro.
DR   InterPro; IPR004275; Brevinin.
DR   Pfam; PF03032; Brevenin; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide;
KW   Cleavage on pair of basic residues; Secreted; Signal.
FT   SIGNAL        1     22       Potential.
FT   PROPEP       23     51
FT                                /FTId=PRO_0000421859.
FT   PEPTIDE      52     64       Kassorin-M.
FT                                /FTId=PRO_5000666360.
FT   MOD_RES      64     64       Leucine amide.
SQ   SEQUENCE   65 AA;  7321 MW;  B2756200A42F50CB CRC64;
     MLTLKKSMLL LFFLGMVSFS LADDKREDEA EEGEDKRADE GEEKRAAEKK RFLEGLLNTV
     TGLLG
//