ID E3GXV7_METFV Unreviewed; 492 AA. AC E3GXV7; DT 11-JAN-2011, integrated into UniProtKB/TrEMBL. DT 11-JAN-2011, sequence version 1. DT 27-MAR-2024, entry version 53. DE SubName: Full=Mur ligase middle domain protein {ECO:0000313|EMBL:ADP77139.1}; GN OrderedLocusNames=Mfer_0336 {ECO:0000313|EMBL:ADP77139.1}; OS Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S). OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria; OC Methanobacteriales; Methanothermaceae; Methanothermus. OX NCBI_TaxID=523846 {ECO:0000313|EMBL:ADP77139.1, ECO:0000313|Proteomes:UP000002315}; RN [1] {ECO:0000313|EMBL:ADP77139.1, ECO:0000313|Proteomes:UP000002315} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 43054 / DSM 2088 / JCM 10308 / V24 S RC {ECO:0000313|Proteomes:UP000002315}; RX PubMed=21304736; RA Anderson I., Djao O.D., Misra M., Chertkov O., Nolan M., Lucas S., RA Lapidus A., Del Rio T.G., Tice H., Cheng J.F., Tapia R., Han C., RA Goodwin L., Pitluck S., Liolios K., Ivanova N., Mavromatis K., RA Mikhailova N., Pati A., Brambilla E., Chen A., Palaniappan K., Land M., RA Hauser L., Chang Y.J., Jeffries C.D., Sikorski J., Spring S., Rohde M., RA Eichinger K., Huber H., Wirth R., Goker M., Detter J.C., Woyke T., RA Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Klenk H.P., RA Kyrpides N.C.; RT "Complete genome sequence of Methanothermus fervidus type strain (V24S)."; RL Stand. Genomic Sci. 3:315-324(2010). RN [2] {ECO:0007829|PDB:6VR7} RP X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH ZN(2+). RX DOI=10.1093/femsmc/xtab012; RA Subedi B.P., Martin W.F., Carbone V., Duin E.C., Cronin B., Sauter J., RA Schofield L.R., Sutherland-Smith A.J., Ronimus R.S.; RT "Archaeal pseudomurein and bacterial murein cell wall biosynthesis share a RT common evolutionary ancestry."; RL FEMS Microbes 2:0-0(2021). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP002278; ADP77139.1; -; Genomic_DNA. DR RefSeq; WP_013413417.1; NC_014658.1. DR PDB; 6VR7; X-ray; 2.50 A; A=1-492. DR AlphaFoldDB; E3GXV7; -. DR SMR; E3GXV7; -. DR STRING; 523846.Mfer_0336; -. DR GeneID; 9962051; -. DR KEGG; mfv:Mfer_0336; -. DR HOGENOM; CLU_012007_0_0_2; -. DR OrthoDB; 75177at2157; -. DR Proteomes; UP000002315; Chromosome. DR GO; GO:0005524; F:ATP binding; IEA:InterPro. DR GO; GO:0004326; F:tetrahydrofolylpolyglutamate synthase activity; IEA:InterPro. DR Gene3D; 3.90.190.20; Mur ligase, C-terminal domain; 1. DR Gene3D; 3.40.1190.10; Mur-like, catalytic domain; 1. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1. DR InterPro; IPR018109; Folylpolyglutamate_synth_CS. DR InterPro; IPR036565; Mur-like_cat_sf. DR InterPro; IPR004101; Mur_ligase_C. DR InterPro; IPR036615; Mur_ligase_C_dom_sf. DR InterPro; IPR013221; Mur_ligase_cen. DR PANTHER; PTHR23135; MUR LIGASE FAMILY MEMBER; 1. DR PANTHER; PTHR23135:SF4; UDP-N-ACETYLMURAMOYL-L-ALANYL-D-GLUTAMATE--2,6-DIAMINOPIMELATE LIGASE MURE HOMOLOG, CHLOROPLASTIC; 1. DR Pfam; PF02875; Mur_ligase_C; 1. DR Pfam; PF08245; Mur_ligase_M; 1. DR SUPFAM; SSF51984; MurCD N-terminal domain; 1. DR SUPFAM; SSF53623; MurD-like peptide ligases, catalytic domain; 1. DR SUPFAM; SSF53244; MurD-like peptide ligases, peptide-binding domain; 1. DR PROSITE; PS01011; FOLYLPOLYGLU_SYNT_1; 1. PE 1: Evidence at protein level; KW 3D-structure {ECO:0007829|PDB:6VR7}; Ligase {ECO:0000313|EMBL:ADP77139.1}; KW Metal-binding {ECO:0007829|PDB:6VR7}; KW Reference proteome {ECO:0000313|Proteomes:UP000002315}; KW Zinc {ECO:0007829|PDB:6VR7}. FT DOMAIN 112..331 FT /note="Mur ligase central" FT /evidence="ECO:0000259|Pfam:PF08245" FT DOMAIN 351..422 FT /note="Mur ligase C-terminal" FT /evidence="ECO:0000259|Pfam:PF02875" FT BINDING 257 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0007829|PDB:6VR7" FT BINDING 260 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0007829|PDB:6VR7" FT BINDING 279 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0007829|PDB:6VR7" FT BINDING 281 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0007829|PDB:6VR7" SQ SEQUENCE 492 AA; 54381 MW; C6C558F4DB20F345 CRC64; MQLKNKKIVV IGGCGTVGSL MARILKSKGN DVTVSDIRKD TYLKDIFKSE GIKLDLGGHD LSLIKKADAI AIAPSLTNNK KVLNLINKNP KAELIKIEDI LKYKVKKPVV GITGTNGKTT TREMLKNILK ISGLEVPEHH LNIQGNTEFI PPLQARLPGD VAVVEIGTFG VKNEIKRSAK NSNVTVGVIT NISRDHLKNI SFQEYVECKK EITEVAKKLV LNADDPIVAS FGNDNTVYYG IENLKIKIKH FFEDRDCPFC GKNLKYEEIF LGHLGKYECE CGFKRPRPTV KAIDVENKKF ILSIDSNEGL VKLEYGGIFN VYNALAAAAA AFILKIDFDK IIEGLNTFKK VPGRMEKIYK KPEIIIDYAH NVAGVKAILQ TIKPKGRLIV VNTISSESGI KEDIKIAKIL SSADILIPAS YSARKASKYT NTTVINVKST EKKFKKGTLG ASKFQVEEAI KKALSYADKN DTILIIGEGG VKYGREIIEK IK //