ID E1BDK7_BOVIN Unreviewed; 777 AA. AC E1BDK7; DT 02-NOV-2010, integrated into UniProtKB/TrEMBL. DT 16-NOV-2011, sequence version 2. DT 24-JAN-2024, entry version 76. DE RecName: Full=Zinc phosphodiesterase ELAC protein 2 {ECO:0000256|ARBA:ARBA00013357}; DE EC=3.1.26.11 {ECO:0000256|ARBA:ARBA00012477}; DE AltName: Full=ElaC homolog protein 2 {ECO:0000256|ARBA:ARBA00032616}; DE AltName: Full=Ribonuclease Z 2 {ECO:0000256|ARBA:ARBA00030729}; DE AltName: Full=tRNA 3 endonuclease 2 {ECO:0000256|ARBA:ARBA00032104}; DE AltName: Full=tRNase Z 2 {ECO:0000256|ARBA:ARBA00030689}; GN Name=ELAC2 {ECO:0000313|Ensembl:ENSBTAP00000029249.5, GN ECO:0000313|VGNC:VGNC:28422}; OS Bos taurus (Bovine). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; OC Bovinae; Bos. OX NCBI_TaxID=9913 {ECO:0000313|Ensembl:ENSBTAP00000029249.5, ECO:0000313|Proteomes:UP000009136}; RN [1] {ECO:0000313|Ensembl:ENSBTAP00000029249.5, ECO:0000313|Proteomes:UP000009136} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000029249.5, RC ECO:0000313|Proteomes:UP000009136}; RA Rosen B.D., Bickhart D.M., Koren S., Schnabel R.D., Hall R., Zimin A., RA Dreischer C., Schultheiss S., Schroeder S.G., Elsik C.G., Couldrey C., RA Liu G.E., Van Tassell C.P., Phillippy A.M., Smith T.P.L., Medrano J.F.; RT "ARS-UCD1.2."; RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|Ensembl:ENSBTAP00000029249.5} RP IDENTIFICATION. RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000029249.5}; RG Ensembl; RL Submitted (JUL-2023) to UniProtKB. CC -!- CATALYTIC ACTIVITY: CC Reaction=Endonucleolytic cleavage of RNA, removing extra 3' nucleotides CC from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy CC group is left at the tRNA terminus and a 5'-phosphoryl group is left CC at the trailer molecule.; EC=3.1.26.11; CC Evidence={ECO:0000256|ARBA:ARBA00000402}; CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Evidence={ECO:0000256|ARBA:ARBA00001947}; CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|ARBA:ARBA00004173}. CC -!- SIMILARITY: Belongs to the RNase Z family. CC {ECO:0000256|ARBA:ARBA00007823}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR RefSeq; NP_001179994.1; NM_001193065.1. DR AlphaFoldDB; E1BDK7; -. DR SMR; E1BDK7; -. DR STRING; 9913.ENSBTAP00000029249; -. DR PaxDb; 9913-ENSBTAP00000029249; -. DR Ensembl; ENSBTAT00000029249.6; ENSBTAP00000029249.5; ENSBTAG00000021939.6. DR GeneID; 509256; -. DR KEGG; bta:509256; -. DR CTD; 60528; -. DR VEuPathDB; HostDB:ENSBTAG00000021939; -. DR VGNC; VGNC:28422; ELAC2. DR eggNOG; KOG2121; Eukaryota. DR GeneTree; ENSGT00730000111191; -. DR HOGENOM; CLU_006220_2_0_1; -. DR InParanoid; E1BDK7; -. DR OMA; INYICQL; -. DR TreeFam; TF105797; -. DR Proteomes; UP000009136; Chromosome 19. DR Bgee; ENSBTAG00000021939; Expressed in corpus luteum and 108 other cell types or tissues. DR GO; GO:0042645; C:mitochondrial nucleoid; IEA:UniProtKB-SubCell. DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central. DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl. DR GO; GO:0042781; F:3'-tRNA processing endoribonuclease activity; IBA:GO_Central. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:1990180; P:mitochondrial tRNA 3'-end processing; IEA:Ensembl. DR CDD; cd07718; RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold; 1. DR Gene3D; 3.60.15.10; Ribonuclease Z/Hydroxyacylglutathione hydrolase-like; 2. DR InterPro; IPR001279; Metallo-B-lactamas. DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro. DR InterPro; IPR047151; RNZ2-like. DR InterPro; IPR027794; tRNase_Z_dom. DR PANTHER; PTHR12553; ZINC PHOSPHODIESTERASE ELAC PROTEIN 2; 1. DR PANTHER; PTHR12553:SF49; ZINC PHOSPHODIESTERASE ELAC PROTEIN 2; 1. DR Pfam; PF12706; Lactamase_B_2; 1. DR Pfam; PF13691; Lactamase_B_4; 1. DR SUPFAM; SSF56281; Metallo-hydrolase/oxidoreductase; 2. PE 3: Inferred from homology; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}; KW Reference proteome {ECO:0000313|Proteomes:UP000009136}; KW Signal {ECO:0000256|SAM:SignalP}; Zinc {ECO:0000256|ARBA:ARBA00022833}. FT SIGNAL 1..19 FT /evidence="ECO:0000256|SAM:SignalP" FT CHAIN 20..777 FT /note="Zinc phosphodiesterase ELAC protein 2" FT /evidence="ECO:0000256|SAM:SignalP" FT /id="PRO_5003143966" FT DOMAIN 57..116 FT /note="tRNase Z endonuclease" FT /evidence="ECO:0000259|Pfam:PF13691" FT DOMAIN 466..679 FT /note="Metallo-beta-lactamase" FT /evidence="ECO:0000259|Pfam:PF12706" FT REGION 17..49 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 748..777 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 27..42 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 777 AA; 86003 MW; 7026F87BCB1BF40D CRC64; MWALRSLLGL RCAAGRTMSQ GPARRPRPPK DPLRHLRTRE KRGPSWGPGG PNTVYLQVVA AGGRDAGAAL YVFSEFNRYL FNCGEGVQRL MQEHKLKVSR LDNIFLTRMH WSNVGGLCGM ILTLKETGVP KCVLSGPPQL EKYLEAIKIF SGPLKGIDLA VRPHSAPEYK DETMTVFQIP IHCVGWKARG RDTSLVVAFV CKLHVKKGNF LVLKAKELGL PVGTAAIAPI IAAVKDGKSV TYEGREILPE EICTPPDPGI TFIVVECPDE GFIQPLCENT TFRSYQGKAD APVALVVHMA PEHVLLDSRY QQWMERFGPD TEHLVLNETC ESVHNLRSHK IQTQLSLIHP GIFPPLAGPR PQEGSATQGV PTVRGQCLLK YQLRPRREWQ RDAVLTCDPE EFIAEALELP NFQESVQEYR KTAQDSPEAT SSQYPEVVFL GTGSAIPMKI RNVSSTLVNI SPDTSLLLDC GEGTFGQLCR HYGDGVDRVL GSLAAVFVSH LHADHHTGLL NILLQRERAL ASLGRPCHPL LVVAPTQLRT WLQQYHNQCQ PLLHHVSVIP AKCLQKGAEV SSPEVERLIN LLLETCGLEE FQTCLVRHCK HAFGCALVHM SGWKVVYSGD TMPCEALVQM GKDATLLIHE ATLEDGLEEE AVEKTHSTTS QAIGVGMRMS AAFIMLTHFS QRYAKIPLFS PDFNEKVGIA FDHMKVSLGD LPTVPRLTAP LKALFAGDLE EMEGRRERRE LRQVRAALLA GEDVEPPQKR APTEHPLSPQ SKKARAQ //