ID D9I6Q2_9INFA Unreviewed; 759 AA. AC D9I6Q2; DT 05-OCT-2010, integrated into UniProtKB/TrEMBL. DT 05-OCT-2010, sequence version 1. DT 29-MAY-2024, entry version 47. DE RecName: Full=Polymerase basic protein 2 {ECO:0000256|ARBA:ARBA00014261, ECO:0000256|RuleBase:RU361246}; DE Flags: Fragment; GN Name=PB2 {ECO:0000256|RuleBase:RU361246, ECO:0000313|EMBL:ADF83731.1}; OS Influenza A virus (A/Vietnam/UT3047III/2004(H5N1)). OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; OC Alphainfluenzavirus influenzae; Influenza A virus. OX NCBI_TaxID=755294 {ECO:0000313|EMBL:ADF83731.1}; RN [1] {ECO:0000313|EMBL:ADF83731.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=A/Vietnam/UT3047III/2004 {ECO:0000313|EMBL:ADF83731.1}; RX PubMed=20592108; DOI=10.1099/vir.0.021659-0; RA Le Q.M., Ito M., Muramoto Y., Hoang P.V., Vuong C.D., Sakai-Tagawa Y., RA Kiso M., Ozawa M., Takano R., Kawaoka Y.; RT "Pathogenicity of highly pathogenic avian H5N1 influenza A viruses isolated RT from humans between 2003 and 2008 in northern Vietnam."; RL J. Gen. Virol. 91:2485-2490(2010). CC -!- FUNCTION: Plays an essential role in transcription initiation and cap- CC stealing mechanism, in which cellular capped pre-mRNAs are used to CC generate primers for viral transcription. CC {ECO:0000256|RuleBase:RU361246}. CC -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1, CC PB2 and PA. {ECO:0000256|RuleBase:RU361246}. CC -!- SUBCELLULAR LOCATION: Host mitochondrion CC {ECO:0000256|ARBA:ARBA00004181}. Mitochondrion CC {ECO:0000256|ARBA:ARBA00004173}. Virion CC {ECO:0000256|RuleBase:RU361246}. Host nucleus CC {ECO:0000256|RuleBase:RU361246}. CC -!- SIMILARITY: Belongs to the influenza viruses PB2 family. CC {ECO:0000256|ARBA:ARBA00008660, ECO:0000256|RuleBase:RU361246}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; HM114494; ADF83731.1; -; Viral_cRNA. DR GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell. DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0044423; C:virion component; IEA:UniProtKB-KW. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW. DR GO; GO:0075526; P:cap snatching; IEA:UniProtKB-KW. DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro. DR GO; GO:0039523; P:symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR Gene3D; 3.30.30.90; Polymerase Basic Protein 2, C-terminal domain; 1. DR HAMAP; MF_04062; INV_PB2; 1. DR InterPro; IPR049110; Flu_PB2_2nd. DR InterPro; IPR049114; Flu_PB2_6th. DR InterPro; IPR049115; Flu_PB2_C. DR InterPro; IPR048298; Flu_PB2_CAP-bd. DR InterPro; IPR049111; Flu_PB2_middle. DR InterPro; IPR049106; Flu_PB2_N. DR InterPro; IPR001591; INV_PB2. DR InterPro; IPR049113; PB2_helical. DR InterPro; IPR037258; PDB2_C. DR Pfam; PF20947; Flu_PB2_1st; 1. DR Pfam; PF20948; Flu_PB2_2nd; 1. DR Pfam; PF20949; Flu_PB2_3rd; 1. DR Pfam; PF20950; Flu_PB2_4th; 1. DR Pfam; PF00604; Flu_PB2_5th; 1. DR Pfam; PF20951; Flu_PB2_6th; 1. DR Pfam; PF20952; Flu_PB2_7th; 1. DR SUPFAM; SSF160453; PB2 C-terminal domain-like; 1. PE 3: Inferred from homology; KW Cap snatching {ECO:0000256|ARBA:ARBA00022715}; KW Eukaryotic host gene expression shutoff by virus KW {ECO:0000256|ARBA:ARBA00023247}; KW Eukaryotic host transcription shutoff by virus KW {ECO:0000256|ARBA:ARBA00022731}; KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995}; KW Host nucleus {ECO:0000256|ARBA:ARBA00022562, KW ECO:0000256|RuleBase:RU361246}; KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581}; KW Inhibition of host RNA polymerase II by virus KW {ECO:0000256|ARBA:ARBA00023103}; KW mRNA capping {ECO:0000256|ARBA:ARBA00023042, KW ECO:0000256|RuleBase:RU361246}; KW mRNA processing {ECO:0000256|ARBA:ARBA00022664, KW ECO:0000256|RuleBase:RU361246}; KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}; KW Virion {ECO:0000256|ARBA:ARBA00022844, ECO:0000256|RuleBase:RU361246}. FT DOMAIN 1..36 FT /note="Influenza RNA polymerase PB2 N-terminal region" FT /evidence="ECO:0000259|Pfam:PF20947" FT DOMAIN 37..104 FT /note="Influenza RNA polymerase PB2 second" FT /evidence="ECO:0000259|Pfam:PF20948" FT DOMAIN 107..248 FT /note="Influenza RNA polymerase PB2 middle" FT /evidence="ECO:0000259|Pfam:PF20949" FT DOMAIN 250..319 FT /note="Polymerase basic protein 2 helical" FT /evidence="ECO:0000259|Pfam:PF20950" FT DOMAIN 320..530 FT /note="Influenza RNA polymerase PB2 CAP binding" FT /evidence="ECO:0000259|Pfam:PF00604" FT DOMAIN 531..680 FT /note="Influenza RNA polymerase PB2 sixth" FT /evidence="ECO:0000259|Pfam:PF20951" FT DOMAIN 683..757 FT /note="Influenza RNA polymerase PB2 C-terminal" FT /evidence="ECO:0000259|Pfam:PF20952" FT NON_TER 759 FT /evidence="ECO:0000313|EMBL:ADF83731.1" SQ SEQUENCE 759 AA; 85745 MW; 44A9381CFBBFE953 CRC64; MERIKELRDL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM MAMKYPITAD KRIIEMIPER NEQGQTLWSK TNDAGSDRVM VSPLAVTWWN RNGPATSAVH YPKVYKTYFE KVERLKHGTF GPVHFRNQVK IRRRVDINPG HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTITKEKK EELQDCKIAP LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEV RNDDVDQSLI IAARNIVRRA TVSADPLASL LEMCHSTQIG GIRMVDILRQ NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKK EEEVLTGNLQ TLKIRVHEGY EEFTMVGRKA TAILRKATRR LIQLIVSGRD EQSIAEAIIV AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG IEPIDNVMGM IGILPDMTPS TEMSLRGVRV SKMGVDEYSS TERVVVSIDR FLRVRDQQGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN GPESVLVNTY QWIIRNWETV KIQWSQDPTM LYNKMEFEPF QSLVPKAARG QYSGFVRTLF QQMRDVLGTF DTVQIIKLLP FAAAPPEQSR MQFSSLTVNV RGSGMRILVR GNSPVFNYNK ATKRLTVLGK DAGALTEDPD EGTAGVESAV LRGFLILGKE NKRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA TKRIRMAIN //