ID NFAT5_RAT Reviewed; 1548 AA. AC D3ZGB1; DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot. DT 20-APR-2010, sequence version 1. DT 06-JUL-2016, entry version 56. DE RecName: Full=Nuclear factor of activated T-cells 5; DE Short=NF-AT5; DE AltName: Full=T-cell transcription factor NFAT5; GN Name=Nfat5; OS Rattus norvegicus (Rat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Rattus. OX NCBI_TaxID=10116; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Brown Norway; RX PubMed=15057822; DOI=10.1038/nature02426; RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., RA Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., RA Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., RA Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., RA Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., RA Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., RA Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., RA Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., RA Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., RA D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., RA Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., RA Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., RA Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., RA Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., RA Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., RA Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., RA Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., RA Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., RA Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., RA Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., RA Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., RA Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., RA Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., RA Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., RA Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., RA Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., RA Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., RA Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., RA Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., RA Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., RA Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., RA Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., RA Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., RA Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., RA Collins F.S.; RT "Genome sequence of the Brown Norway rat yields insights into RT mammalian evolution."; RL Nature 428:493-521(2004). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.; RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. RN [3] RP FUNCTION, INTERACTION WITH CIDEC, SUBCELLULAR LOCATION, TISSUE RP SPECIFICITY, AND INDUCTION BY OSMOTIC STRESS. RX PubMed=23233732; DOI=10.1194/jlr.M033365; RA Ueno M., Shen W.J., Patel S., Greenberg A.S., Azhar S., Kraemer F.B.; RT "Fat-specific protein 27 modulates nuclear factor of activated T cells RT 5 and the cellular response to stress."; RL J. Lipid Res. 54:734-743(2013). CC -!- FUNCTION: Transcription factor involved in the transcriptional CC regulation of osmoprotective and inflammatory genes. Regulates CC hypertonicity-induced cellular accumulation of osmolytes. CC {ECO:0000269|PubMed:23233732}. CC -!- SUBUNIT: Homodimer when bound to DNA, completely encircles its DNA CC target. Does not bind with Fos and Jun transcription factors (By CC similarity). Interacts with CIDEC; this interaction is direct and CC retains NFAT5 in the cytoplasm. {ECO:0000250, CC ECO:0000269|PubMed:23233732}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23233732}. CC Cytoplasm {ECO:0000269|PubMed:23233732}. Note=Nuclear distribution CC increases under hypertonic conditions. CC -!- INDUCTION: Up-regulated under hypertonic conditions. CC {ECO:0000269|PubMed:23233732}. CC -!- PTM: Phosphorylated at Thr-152 by CDK5 in response to osmotic CC stress; this phosphorylation mediates its rapid nuclear CC localization. {ECO:0000250}. CC -!- SIMILARITY: Contains 1 RHD (Rel-like) domain. CC {ECO:0000255|PROSITE-ProRule:PRU00265}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AABR06098525; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AABR06098526; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AABR06098527; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH473972; EDL92476.1; -; Genomic_DNA. DR RefSeq; NP_001100895.1; NM_001107425.1. DR UniGene; Rn.203336; -. DR UniGene; Rn.22934; -. DR ProteinModelPortal; D3ZGB1; -. DR BioGrid; 258819; 1. DR STRING; 10116.ENSRNOP00000017005; -. DR iPTMnet; D3ZGB1; -. DR PaxDb; D3ZGB1; -. DR Ensembl; ENSRNOT00000017005; ENSRNOP00000017005; ENSRNOG00000011879. DR GeneID; 307820; -. DR KEGG; rno:307820; -. DR CTD; 10725; -. DR RGD; 1309142; Nfat5. DR eggNOG; ENOG410IGY4; Eukaryota. DR eggNOG; ENOG41118Z9; LUCA. DR GeneTree; ENSGT00550000074562; -. DR InParanoid; D3ZGB1; -. DR KO; K17335; -. DR OMA; FGIQNNC; -. DR OrthoDB; EOG79PJND; -. DR TreeFam; TF326480; -. DR PRO; PR:D3ZGB1; -. DR Proteomes; UP000002494; Chromosome 19. DR ExpressionAtlas; D3ZGB1; baseline and differential. DR Genevisible; D3ZGB1; RN. DR GO; GO:0005737; C:cytoplasm; IDA:RGD. DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl. DR GO; GO:0005634; C:nucleus; IDA:RGD. DR GO; GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IEA:Ensembl. DR GO; GO:0001077; F:transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding; IEA:Ensembl. DR GO; GO:0001816; P:cytokine production; IEA:Ensembl. DR GO; GO:0070884; P:regulation of calcineurin-NFAT signaling cascade; IEA:Ensembl. DR GO; GO:0006970; P:response to osmotic stress; IEP:RGD. DR Gene3D; 2.60.40.10; -; 1. DR Gene3D; 2.60.40.340; -; 1. DR InterPro; IPR013783; Ig-like_fold. DR InterPro; IPR014756; Ig_E-set. DR InterPro; IPR002909; IPT. DR InterPro; IPR008366; NFAT. DR InterPro; IPR015646; NFAT5. DR InterPro; IPR008967; p53-like_TF_DNA-bd. DR InterPro; IPR032397; RHD_dimer. DR InterPro; IPR011539; RHD_DNA_bind_dom. DR PANTHER; PTHR12533; PTHR12533; 4. DR PANTHER; PTHR12533:SF10; PTHR12533:SF10; 4. DR Pfam; PF16179; RHD_dimer; 1. DR Pfam; PF00554; RHD_DNA_bind; 1. DR PRINTS; PR01789; NUCFACTORATC. DR SMART; SM00429; IPT; 1. DR SUPFAM; SSF49417; SSF49417; 1. DR SUPFAM; SSF81296; SSF81296; 1. DR PROSITE; PS50254; REL_2; 1. PE 1: Evidence at protein level; KW Acetylation; Activator; Complete proteome; Cytoplasm; DNA-binding; KW Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; KW Transcription; Transcription regulation; Ubl conjugation. FT CHAIN 1 1548 Nuclear factor of activated T-cells 5. FT /FTId=PRO_0000423618. FT DOMAIN 281 460 RHD. {ECO:0000255|PROSITE- FT ProRule:PRU00265}. FT DNA_BIND 310 317 {ECO:0000250}. FT COMPBIAS 678 1511 Gln-rich. FT MOD_RES 137 137 Phosphoserine. FT {ECO:0000250|UniProtKB:O94916}. FT MOD_RES 139 139 N6-acetyllysine. FT {ECO:0000250|UniProtKB:Q9WV30}. FT MOD_RES 151 151 Phosphoserine. FT {ECO:0000250|UniProtKB:O94916}. FT MOD_RES 152 152 Phosphothreonine; by CDK5. {ECO:0000250}. FT MOD_RES 172 172 Phosphoserine. FT {ECO:0000250|UniProtKB:O94916}. FT MOD_RES 577 577 Phosphoserine. FT {ECO:0000250|UniProtKB:O94916}. FT CROSSLNK 572 572 Glycyl lysine isopeptide (Lys-Gly) FT (interchain with G-Cter in SUMO1). FT {ECO:0000250|UniProtKB:O94916}. FT CROSSLNK 572 572 Glycyl lysine isopeptide (Lys-Gly) FT (interchain with G-Cter in SUMO2). FT {ECO:0000250|UniProtKB:O94916}. SQ SEQUENCE 1548 AA; 167207 MW; CE3F4A41532770EF CRC64; MPSDFISLLS ADLDLESPKS LYSRDSLKLH PSQNFHRAGL LEESVYDLLP KELQLPPPRE TSAASMSQTS GGEAGSPPPA VVAADASSAP SSSMGGACSS FTTSSSPTIY STSVTDSKAM QVESCSSAVG VSNRGVSEKQ LTGNTVQQHP STPKRHTVLY ISPPPEDLLD NSRMSCQDEG CGLESEQSCS MWMEDSPSNF SNMSTSSYND NTEVPRKSRK RNPKQRPGVK RRDCEESNMD IFDADSAKAP HYVLSQLTTD NKGNSKAGNG TLDSQKGTGV KKSPMLCGQY PVKSEGKELK IVVQPETQHR ARYLTEGSRG SVKDRTQQGF PTVKLEGHNE PVVLQVFVGN DSGRVKPHGF YQACRVTGRN TTPCKEVDIE GTTVIEVGLD PSSNMTLAVD CVGILKLRNA DVEARIGIAG SKKKSTRARL VFRVNITRKD GSTLTLQTPS SPILCTQPAG VPEILKKSLH SCSVKGEEEV FLIGKNFLKG TKVIFQENVS DENSWKSEAE IDMELFHQNH LIVKVPPYHD QHITLPVSVG IYVVTNAGRS HDVQPFTYTP DPAAGALSVN VKKEISSPAR PCSFEEALKA MKTTGCNVDK VTILPNALIT PLISSSMIKT EDVTPMEVTS EKRSSPIFQT TKTVGSTQQT LETISNIAGN ASFSSPSSSH LSPENENQQQ LQPKAYNPET LTTIQTQDIS QPGTFPAVSA SSQLPSSDAL LQQAAQFQTR DAQSRDTMQS DSVVNLSQLT EAPQQQQSPL QEQAQIPSNI FPSPNSVSQL QSTIQQLQAG SFTGSTASGS NGSVDLVQQV LEAQQQLSSV LFSTPDGNEN VQEQLNADIF QVSQIQNSVS PGMFSSTESA VHTRPDNLLP GRADSVHQQT ENTLSSQQQQ QQQQQQQQQQ QVIESSAAMV MEMQQSICQA AAQIQSELFP SAASANGSLQ QSPVYQQPSH MMSALPTSED MQMQCELFSS PPAVSGNETS TTTTPQVATP GSTMFQPPNS GDGEETGAQA KQIQSSVFQT MVQMQHSGDS QPQVNLFSST KNIMSVQSNG TQQQGNSLFQ QGSEMLSLQS GSFLQQSSHS QAQLFHPQNP IADAQSLSQE TQGPMFHSAN PIVHSQTSTA SSEQLQPSMF HSQSTIAVLQ GSSVPQDQQS PNIYLSQSSI SNLQTNTVAQ EEQISFFSAQ NSISPLQSTS NTEQQAAFQQ QPPISHIQTP LLSQEQAQPS QQGLFQPQVA LGSLPANPMP QNQQGPIFQT QRPIVGMQSN SPSQEQQQQQ QQQQQQQQQQ QQQSILFSNQ NAMATMASQK QPPPNMIFSP NQNPMASQEQ QNQSIFHQQS NMAPMNQEQQ PMQFQNQPTV SSLQNPGPTP SESPQTSLFH SSPQIQLVQG SPSSQEQQVT LFLSPASMSA LQTSINQPDM QQSPLYSPQN NIPGIQGSTS SPQPQAALFH NTTGGTINQL QNSPGSSQQT SGMFLFGIQN NCSQLLTSGP ATLPEQLMAI NQPGQPQNEG QSSVTTLLSQ QMPESAPLAS SVNNSQNMEK IDLLVSLQSQ GNNLTGSF //