ID MTRA2_METRM Reviewed; 193 AA. AC D3E0P6; DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot. DT 23-MAR-2010, sequence version 1. DT 28-FEB-2018, entry version 43. DE RecName: Full=Tetrahydromethanopterin S-methyltransferase subunit A 2 {ECO:0000255|HAMAP-Rule:MF_01093}; DE EC=2.1.1.86 {ECO:0000255|HAMAP-Rule:MF_01093}; DE AltName: Full=N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit A 2 {ECO:0000255|HAMAP-Rule:MF_01093}; GN Name=mtrA2 {ECO:0000255|HAMAP-Rule:MF_01093}; GN OrderedLocusNames=mru_0441; OS Methanobrevibacter ruminantium (strain ATCC 35063 / DSM 1093 / JCM OS 13430 / OCM 146 / M1) (Methanobacterium ruminantium). OC Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; OC Methanobacteriaceae; Methanobrevibacter. OX NCBI_TaxID=634498; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 35063 / DSM 1093 / JCM 13430 / OCM 146 / M1; RX PubMed=20126622; DOI=10.1371/journal.pone.0008926; RA Leahy S.C., Kelly W.J., Altermann E., Ronimus R.S., Yeoman C.J., RA Pacheco D.M., Li D., Kong Z., McTavish S., Sang C., Lambie S.C., RA Janssen P.H., Dey D., Attwood G.T.; RT "The genome sequence of the rumen methanogen Methanobrevibacter RT ruminantium reveals new possibilities for controlling ruminant methane RT emissions."; RL PLoS ONE 5:E8926-E8926(2010). CC -!- FUNCTION: Part of a complex that catalyzes the formation of CC methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and CC methyl-tetrahydromethanopterin. This is an energy-conserving, CC sodium-ion translocating step. {ECO:0000255|HAMAP-Rule:MF_01093}. CC -!- CATALYTIC ACTIVITY: 5-methyl-5,6,7,8-tetrahydromethanopterin + CC coenzyme M + 2 Na(+)(In) = 5,6,7,8-tetrahydromethanopterin + 2- CC (methylsulfanyl)ethane-1-sulfonate + 2 Na(+)(Out). CC {ECO:0000255|HAMAP-Rule:MF_01093}. CC -!- COFACTOR: CC Name=5-hydroxybenzimidazolylcob(I)amide; Xref=ChEBI:CHEBI:60494; CC Evidence={ECO:0000255|HAMAP-Rule:MF_01093}; CC Note=Binds 1 5-hydroxybenzimidazolylcobamide group. CC {ECO:0000255|HAMAP-Rule:MF_01093}; CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl- CC coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: CC step 2/2. {ECO:0000255|HAMAP-Rule:MF_01093}. CC -!- SUBUNIT: The complex is composed of 8 subunits; MtrA, MtrB, MtrC, CC MtrD, MtrE, MtrF, MtrG and MtrH. {ECO:0000255|HAMAP- CC Rule:MF_01093}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP- CC Rule:MF_01093}; Single-pass membrane protein {ECO:0000255|HAMAP- CC Rule:MF_01093}. CC -!- SIMILARITY: Belongs to the MtrA family. {ECO:0000255|HAMAP- CC Rule:MF_01093}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP001719; ADC46292.1; -; Genomic_DNA. DR RefSeq; WP_012955243.1; NC_013790.1. DR SMR; D3E0P6; -. DR STRING; 634498.mru_0441; -. DR EnsemblBacteria; ADC46292; ADC46292; mru_0441. DR GeneID; 8770081; -. DR KEGG; mru:mru_0441; -. DR PATRIC; fig|634498.28.peg.443; -. DR eggNOG; arCOG03221; Archaea. DR eggNOG; COG4063; LUCA. DR HOGENOM; HOG000229674; -. DR KO; K00577; -. DR OMA; QRVGRKI; -. DR OrthoDB; POG093Z0GAB; -. DR BioCyc; MRUM634498:G1GFU-450-MONOMER; -. DR UniPathway; UPA00640; UER00698. DR Proteomes; UP000008680; Chromosome. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0050897; F:cobalt ion binding; IEA:InterPro. DR GO; GO:0030269; F:tetrahydromethanopterin S-methyltransferase activity; IEA:UniProtKB-EC. DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway. DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW. DR GO; GO:0006814; P:sodium ion transport; IEA:InterPro. DR HAMAP; MF_01093; MtrA; 1. DR InterPro; IPR030688; MeTrfase_MtrA/MtxA. DR InterPro; IPR005778; MtrA. DR Pfam; PF04208; MtrA; 1. DR PIRSF; PIRSF500207; MtrA; 1. DR PIRSF; PIRSF009452; MtrA_MtxA; 1. DR TIGRFAMs; TIGR01111; mtrA; 1. PE 3: Inferred from homology; KW Cell membrane; Cobalt; Complete proteome; Membrane; Methanogenesis; KW Methyltransferase; One-carbon metabolism; Reference proteome; KW Transferase; Transmembrane; Transmembrane helix. FT CHAIN 1 193 Tetrahydromethanopterin S- FT methyltransferase subunit A 2. FT /FTId=PRO_0000403060. FT TOPO_DOM 1 38 Cytoplasmic. {ECO:0000255|HAMAP- FT Rule:MF_01093}. FT TRANSMEM 39 58 Helical. {ECO:0000255|HAMAP- FT Rule:MF_01093}. FT TOPO_DOM 59 193 Extracellular. {ECO:0000255|HAMAP- FT Rule:MF_01093}. FT BINDING 84 84 5'-hydroxybenzimidazolyl-cobamide FT cofactor. {ECO:0000255|HAMAP- FT Rule:MF_01093}. SQ SEQUENCE 193 AA; 20713 MW; D126A067DFD95C74 CRC64; MADKKPTAEN WPVVSGDYIV GDPESPVAVT TLASHNEDIP AAAGAAIAGP CKTENLGIEK VVANIISNPN IRFLILCGAE VQGHITGQSF KALYENGCDP EKKKITGATG AIPFVENIPM EGVERFQQQL ELVDMIDNED GGAITAKVKE CIEKDPGAFE EDSLVIKIDE ERYSKKSSFV ESSSESEKIE SEA //