ID D1CD35_THET1 Unreviewed; 342 AA. AC D1CD35; DT 19-JAN-2010, integrated into UniProtKB/TrEMBL. DT 19-JAN-2010, sequence version 1. DT 14-MAY-2014, entry version 32. DE RecName: Full=6-phosphofructokinase; DE Short=Phosphofructokinase; DE EC=2.7.1.11; DE AltName: Full=Phosphohexokinase; GN Name=pfkA; OrderedLocusNames=Tter_1794; OS Thermobaculum terrenum (strain ATCC BAA-798 / YNP1). OC Bacteria; Thermobaculum. OX NCBI_TaxID=525904; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC BAA-798 / YNP1; RX DOI=10.4056/sigs.1153107; RA Kiss H., Cleland D., Lapidus A., Lucas S., Glavina Del Rio T., RA Nolan M., Tice H., Han C., Goodwin L., Pitluck S., Liolios K., RA Ivanova N., Mavromatis K., Ovchinnikova G., Pati A., Chen A., RA Palaniappan K., Land M., Hauser L., Chang Y., Jeffries C., Lu M., RA Brettin T., Detter J., Goker M., Tindall B., Beck B., McDermott T., RA Woyke T., Bristow J., Eisen J., Markowitz V., Hugenholtz P., RA Kyrpides N., Klenk H., Cheng J.; RT "Complete genome sequence of Thermobaculum terrenum type strain RT (YNP1T)."; RL Stand. Genomic Sci. 3:153-162(2010). CC -!- CATALYTIC ACTIVITY: ATP + D-fructose 6-phosphate = ADP + D- CC fructose 1,6-bisphosphate. CC -!- COFACTOR: Magnesium (By similarity). CC -!- ENZYME REGULATION: Subject to allosteric activation by ADP and CC other diphosphonucleosides, and inhibition by phosphoenolpyruvate CC (By similarity). CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3- CC phosphate and glycerone phosphate from D-glucose: step 3/4. CC -!- SUBUNIT: Homotetramer (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the phosphofructokinase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP001825; ACZ42700.1; -; Genomic_DNA. DR RefSeq; YP_003323522.1; NC_013525.1. DR ProteinModelPortal; D1CD35; -. DR EnsemblBacteria; ACZ42700; ACZ42700; Tter_1794. DR GeneID; 8646305; -. DR KEGG; ttr:Tter_1794; -. DR PATRIC; 32503912; VBITheTer68767_1921. DR HOGENOM; HOG000248869; -. DR KO; K00850; -. DR OMA; NEICKAM; -. DR OrthoDB; EOG644ZRM; -. DR BioCyc; TTER525904:GHMJ-1850-MONOMER; -. DR UniPathway; UPA00109; UER00182. DR GO; GO:0005945; C:6-phosphofructokinase complex; IEA:InterPro. DR GO; GO:0003872; F:6-phosphofructokinase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0006002; P:fructose 6-phosphate metabolic process; IEA:InterPro. DR GO; GO:0006096; P:glycolysis; IEA:UniProtKB-HAMAP. DR HAMAP; MF_00339; Phosphofructokinase; 1. DR InterPro; IPR012003; ATP_PFK_prok. DR InterPro; IPR022953; Phosphofructokinase. DR InterPro; IPR000023; Phosphofructokinase_dom. DR Pfam; PF00365; PFK; 1. DR PIRSF; PIRSF000532; ATP_PFK_prok; 1. DR PRINTS; PR00476; PHFRCTKINASE. DR SUPFAM; SSF53784; SSF53784; 1. PE 3: Inferred from homology; KW Allosteric enzyme; ATP-binding; Complete proteome; Cytoplasm; KW Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; KW Transferase. FT NP_BIND 71 72 ATP (By similarity). FT REGION 124 126 Substrate binding (By similarity). FT REGION 168 170 Substrate binding (By similarity). FT REGION 184 186 Allosteric activator ADP binding (By FT similarity). FT REGION 272 275 Substrate binding (By similarity). FT ACT_SITE 126 126 Proton acceptor (By similarity). FT METAL 102 102 Magnesium; catalytic (By similarity). FT BINDING 10 10 ATP; via amide nitrogen (By similarity). FT BINDING 153 153 Allosteric activator ADP (By similarity). FT BINDING 161 161 Substrate; shared with dimeric partner FT (By similarity). FT BINDING 210 210 Allosteric activator ADP (By similarity). FT BINDING 221 221 Substrate (By similarity). FT BINDING 266 266 Substrate; shared with dimeric partner FT (By similarity). SQ SEQUENCE 342 AA; 36855 MW; 628C628D7B1BA090 CRC64; MKVGVLTGGG DAPGLNAAIR AVVLSALSRG DEVVGFLDGW KGVQDNLTIP LDREWVQDIH EVGGTNLGTS RTNPMKSEDT MKAVENHFRE NGLDVLIAIG GDDTLSVAAE LDRRGFPVVG VPKTMDNDVP ETDYCIGFDT AVNRLMESID RLRTTSRSHH RVMVVEAMGR DAGWVAGFGG LAGGADVILV PEIEVDLDDV AQRLEKVRSR GRSYAIVVAA EGISFGEAQV PENAKTDAFG HVILAEKAVG ERLAQEIESR LGWETRSIQI GHLHRGGSPT AFDRILGTRY GEKAVELAHN NSFGQMVVLH GLDLTTVPLA QIAGKTKTLY PQFIELIQNL NA //