ID C9RMT1_FIBSS Unreviewed; 894 AA. AC C9RMT1; DT 24-NOV-2009, integrated into UniProtKB/TrEMBL. DT 24-NOV-2009, sequence version 1. DT 27-MAR-2024, entry version 75. DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900}; DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900}; GN OrderedLocusNames=FSU_1135 {ECO:0000313|EMBL:ADL24987.1}; OS Fibrobacter succinogenes (strain ATCC 19169 / S85). OC Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales; Fibrobacteraceae; OC Fibrobacter. OX NCBI_TaxID=59374 {ECO:0000313|EMBL:ADL24987.1, ECO:0000313|Proteomes:UP000000517}; RN [1] {ECO:0000313|Proteomes:UP000000517} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 19169 / S85 {ECO:0000313|Proteomes:UP000000517}; RA Durkin A.S., Nelson K.E., Morrison M., Forsberg C.W., Wilson D.B., RA Russell J.B., Cann I.K.O., Mackie R.I., White B.A.; RT "Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85."; RL Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L- CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA- CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72; CC Evidence={ECO:0000256|ARBA:ARBA00001279}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP002158; ADL24987.1; -; Genomic_DNA. DR RefSeq; WP_014545471.1; NC_017448.1. DR AlphaFoldDB; C9RMT1; -. DR STRING; 59374.FSU_1135; -. DR REBASE; 22227; Fsu85ORF699P. DR REBASE; 27332; FssORF1135P. DR GeneID; 34755056; -. DR KEGG; fsc:FSU_1135; -. DR PATRIC; fig|59374.8.peg.1096; -. DR eggNOG; COG0286; Bacteria. DR HOGENOM; CLU_015410_1_0_0; -. DR OrthoDB; 9814572at2; -. DR Proteomes; UP000000517; Chromosome. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW. DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro. DR GO; GO:0006306; P:DNA methylation; IEA:InterPro. DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW. DR Gene3D; 3.90.220.20; DNA methylase specificity domains; 1. DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1. DR InterPro; IPR003356; DNA_methylase_A-5. DR InterPro; IPR044946; Restrct_endonuc_typeI_TRD_sf. DR InterPro; IPR029063; SAM-dependent_MTases_sf. DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1. DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1. DR Pfam; PF02384; N6_Mtase; 2. DR SUPFAM; SSF116734; DNA methylase specificity domain; 1. DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1. PE 4: Predicted; KW Coiled coil {ECO:0000256|SAM:Coils}; KW DNA-binding {ECO:0000256|ARBA:ARBA00023125}; KW Endonuclease {ECO:0000313|EMBL:ADL24987.1}; KW Hydrolase {ECO:0000313|EMBL:ADL24987.1}; KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, KW ECO:0000313|EMBL:ADL24987.1}; Nuclease {ECO:0000313|EMBL:ADL24987.1}; KW Restriction system {ECO:0000256|ARBA:ARBA00022747}; KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ADL24987.1}. FT DOMAIN 353..481 FT /note="DNA methylase adenine-specific" FT /evidence="ECO:0000259|Pfam:PF02384" FT DOMAIN 487..582 FT /note="DNA methylase adenine-specific" FT /evidence="ECO:0000259|Pfam:PF02384" FT COILED 172..206 FT /evidence="ECO:0000256|SAM:Coils" SQ SEQUENCE 894 AA; 101419 MW; 0A80BF8119D47AC7 CRC64; MAKSIEEQVE DWCKNQLENY FTKTESINSE IDKALKTAPS KKGGSGQNLP DIKCFVSVGF RNIPVMIECK GTKGDFAKTD ENGLISNTNK KGETDYTAIT KYAVNGAIHY AKSILDFTET YKETIAIGVN GYKQDNDLQL EIGVYYLSKD NLAIPKKVGD FSDLSFLKKK NLKEFFEKID ELKLTLEEIE SRKLALEDEI ETKLKRLNQT MHDDLSITVK SRVMLIVGLI MAGLGVEDVS EGLKVEELKG ELGPKSNDAQ KIINKVEDFL RMKNLPEEKR EAIMHELSNV FKNEDLYKPK NGESKLRKVY VIVHKDILPY LTSDLPNIDF TGRLFNVLND WVDVPDGAEN DVVLTPRYVT ELMAKLTEVN MNSYVWDYAT GSAGFLISAM HLMIADAKNK IKSPEELRKT IAKIKAEKLL GIEKLPEIYI LAVLNMILMG DGSSNIINGD STQFDGKYKQ GKMKDKEFPA NVFLLNPPYS APGKGLNFVE KALSKMKSGK AAVLIQENAG STQGDGYTKK ILNKNTLIAS IHMSTDLFIG KSSVQTAIYV FDVGIPHDTE KLVKFIDFSN DGYARQNRKK SSQSVNLKDA DNAKERYAEL VNLVVRGKGK DDKNLNYYKD CYVEDYITSE GNDWTYSQHK KIDIKPTEND FKKIIKEYMA WQISNLIRNE DIYPWTNKSP EQSNCILSKK ERDCIQMKNA TFKAITIGSL FDIHPTKAYK ATNKDLFKEK GKVPVVANSS VDNGIGGWTN LKATEKGNKV VFSDTTTSDS IFYQPNDFVG YPHVQGLYPY CNKWDENSLL YFITCFRKSA SGLFNYGNKF TRKNALKMKV FLPEKNKEDI DFSFMETFIS AQKKLIIQKL SCWLEQQKIN NEIYIIEKEI PQDTLSMIAE KKHH //