ID C8XPB1_ZIKV Unreviewed; 3417 AA. AC C8XPB1; DT 03-NOV-2009, integrated into UniProtKB/TrEMBL. DT 03-NOV-2009, sequence version 1. DT 11-MAY-2016, entry version 57. DE RecName: Full=Genome polyprotein {ECO:0000256|SAAS:SAAS00058068}; OS Zika virus (strain Mr 766) (ZIKV). OC Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; OC Flaviviridae; Flavivirus. OX NCBI_TaxID=64320 {ECO:0000313|EMBL:ABI54475.1}; RN [1] {ECO:0000313|EMBL:ABI54475.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=MR 766 {ECO:0000313|EMBL:ABI54475.1}; RA Brisbarre N., Moureau G., Grard G., de Lamballerie X.; RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:ABI54475.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=MR 766 {ECO:0000313|EMBL:ABI54475.1}; RX PubMed=19741066; DOI=10.1099/vir.0.014506-0; RA Grard G., Moureau G., Charrel R.N., Holmes E.C., Gould E.A., RA de Lamballerie X.; RT "Genomics and evolution of Aedes-borne flaviviruses."; RL J. Gen. Virol. 91:87-94(2010). CC -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. CC {ECO:0000256|SAAS:SAAS00291628}. CC -!- CATALYTIC ACTIVITY: NTP + H(2)O = NDP + phosphate. CC {ECO:0000256|SAAS:SAAS00291428}. CC -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate CC + RNA(n+1). {ECO:0000256|SAAS:SAAS00510455}. CC -!- SUBCELLULAR LOCATION: Virion membrane CC {ECO:0000256|SAAS:SAAS00554409}; Multi-pass membrane protein CC {ECO:0000256|SAAS:SAAS00554409}. CC -!- SIMILARITY: Contains RdRp catalytic domain. CC {ECO:0000256|SAAS:SAAS00510369}. CC -!- SIMILARITY: Contains helicase ATP-binding domain. CC {ECO:0000256|SAAS:SAAS00252625}. CC -!- SIMILARITY: Contains helicase C-terminal domain. CC {ECO:0000256|SAAS:SAAS00251432}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; DQ859059; ABI54475.1; -; Genomic_RNA. DR ProteinModelPortal; C8XPB1; -. DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-KW. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW. DR GO; GO:0019031; C:viral envelope; IEA:InterPro. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro. DR GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro. DR GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:InterPro. DR GO; GO:0004483; F:mRNA (nucleoside-2'-O-)-methyltransferase activity; IEA:InterPro. DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro. DR GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro. DR GO; GO:0070008; F:serine-type exopeptidase activity; IEA:InterPro. DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro. DR GO; GO:0039654; P:fusion of virus membrane with host endosome membrane; IEA:UniProtKB-KW. DR GO; GO:0039520; P:induction by virus of host autophagy; IEA:UniProtKB-KW. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW. DR Gene3D; 2.60.40.350; -; 1. DR Gene3D; 2.60.98.10; -; 2. DR Gene3D; 3.30.387.10; -; 1. DR Gene3D; 3.40.50.150; -; 1. DR Gene3D; 3.40.50.300; -; 2. DR InterPro; IPR011492; DEAD_Flavivir. DR InterPro; IPR000069; Env_glycoprot_M_flavivir. DR InterPro; IPR013755; Flav_gly_cen_dom_subdom1. DR InterPro; IPR001122; Flavi_capsidC. DR InterPro; IPR026470; Flavi_E_Stem/Anchor_dom. DR InterPro; IPR001157; Flavi_NS1. DR InterPro; IPR000752; Flavi_NS2A. DR InterPro; IPR000487; Flavi_NS2B. DR InterPro; IPR000404; Flavi_NS4A. DR InterPro; IPR001528; Flavi_NS4B. DR InterPro; IPR002535; Flavi_propep. DR InterPro; IPR000336; Flavivir/Alphavir_Ig-like. DR InterPro; IPR001850; Flavivirus_NS3_S7. DR InterPro; IPR027287; Flavovir_Ig-like. DR InterPro; IPR014412; Gen_Poly_FLV. DR InterPro; IPR011998; Glycoprot_cen/dimer. DR InterPro; IPR013754; GlyE_dim. DR InterPro; IPR014001; Helicase_ATP-bd. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR014756; Ig_E-set. DR InterPro; IPR026490; mRNA_cap_0/1_MeTrfase. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR009003; Peptidase_S1_PA. DR InterPro; IPR000208; RNA-dir_pol_flavivirus. DR InterPro; IPR007094; RNA-dir_pol_PSvirus. DR InterPro; IPR002877; rRNA_MeTrfase_FtsJ_dom. DR InterPro; IPR029063; SAM-dependent_MTases. DR Pfam; PF01003; Flavi_capsid; 1. DR Pfam; PF07652; Flavi_DEAD; 1. DR Pfam; PF02832; Flavi_glycop_C; 1. DR Pfam; PF00869; Flavi_glycoprot; 1. DR Pfam; PF01004; Flavi_M; 1. DR Pfam; PF00948; Flavi_NS1; 1. DR Pfam; PF01005; Flavi_NS2A; 1. DR Pfam; PF01002; Flavi_NS2B; 1. DR Pfam; PF01350; Flavi_NS4A; 1. DR Pfam; PF01349; Flavi_NS4B; 1. DR Pfam; PF00972; Flavi_NS5; 1. DR Pfam; PF01570; Flavi_propep; 1. DR Pfam; PF01728; FtsJ; 1. DR Pfam; PF00949; Peptidase_S7; 1. DR PIRSF; PIRSF003817; Gen_Poly_FLV; 1. DR SMART; SM00487; DEXDc; 1. DR SMART; SM00490; HELICc; 1. DR SUPFAM; SSF50494; SSF50494; 1. DR SUPFAM; SSF52540; SSF52540; 2. DR SUPFAM; SSF53335; SSF53335; 1. DR SUPFAM; SSF56983; SSF56983; 1. DR SUPFAM; SSF81296; SSF81296; 1. DR TIGRFAMs; TIGR04240; flavi_E_stem; 1. DR PROSITE; PS51527; FLAVIVIRUS_NS2B; 1. DR PROSITE; PS51528; FLAVIVIRUS_NS3PRO; 1. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1. DR PROSITE; PS51194; HELICASE_CTER; 1. DR PROSITE; PS50507; RDRP_SSRNA_POS; 1. DR PROSITE; PS51591; RNA_CAP01_NS5_MT; 1. PE 4: Predicted; KW Activation of host autophagy by virus {ECO:0000256|SAAS:SAAS00445756}; KW ATP-binding {ECO:0000256|SAAS:SAAS00510945}; KW Capsid protein {ECO:0000256|SAAS:SAAS00445860}; KW Disulfide bond {ECO:0000256|SAAS:SAAS00440082}; KW Fusion of virus membrane with host endosomal membrane KW {ECO:0000256|SAAS:SAAS00440227}; KW Fusion of virus membrane with host membrane KW {ECO:0000256|SAAS:SAAS00440065}; KW Helicase {ECO:0000256|SAAS:SAAS00445774}; KW Host membrane {ECO:0000256|SAAS:SAAS00445977}; KW Host-virus interaction {ECO:0000256|SAAS:SAAS00445875}; KW Hydrolase {ECO:0000256|SAAS:SAAS00510997}; KW Membrane {ECO:0000256|SAAS:SAAS00445865, ECO:0000256|SAM:Phobius}; KW Nucleotide-binding {ECO:0000256|SAAS:SAAS00510884}; KW Nucleotidyltransferase {ECO:0000256|SAAS:SAAS00510590}; KW Protease {ECO:0000256|SAAS:SAAS00440231}; KW RNA-binding {ECO:0000256|SAAS:SAAS00463221}; KW RNA-directed RNA polymerase {ECO:0000256|SAAS:SAAS00510600}; KW Serine protease {ECO:0000256|SAAS:SAAS00440108}; KW Transferase {ECO:0000256|SAAS:SAAS00510371}; KW Transmembrane {ECO:0000256|SAAS:SAAS00445861, KW ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|SAAS:SAAS00445939, KW ECO:0000256|SAM:Phobius}; KW Viral attachment to host cell {ECO:0000256|SAAS:SAAS00445995}; KW Viral penetration into host cytoplasm {ECO:0000256|SAAS:SAAS00440098}; KW Viral RNA replication {ECO:0000256|SAAS:SAAS00511060}; KW Virion {ECO:0000256|SAAS:SAAS00445868}; KW Virus entry into host cell {ECO:0000256|SAAS:SAAS00445755}. FT TRANSMEM 250 269 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 275 295 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 740 761 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 767 792 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 1172 1191 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 1267 1284 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 1337 1360 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 1372 1388 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 1394 1411 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 1467 1490 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 2168 2187 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 2194 2210 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 2216 2233 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 2245 2263 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 2300 2321 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 2328 2347 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 2367 2386 Helical. {ECO:0000256|SAM:Phobius}. FT TRANSMEM 2436 2456 Helical. {ECO:0000256|SAM:Phobius}. FT DOMAIN 1367 1496 FLAVIVIRUS_NS2B. {ECO:0000259|PROSITE: FT PS51527}. FT DOMAIN 1497 1674 Peptidase S7. {ECO:0000259|PROSITE: FT PS51528}. FT DOMAIN 1677 1833 Helicase ATP-binding. FT {ECO:0000259|PROSITE:PS51192}. FT DOMAIN 1828 2007 Helicase C-terminal. FT {ECO:0000259|PROSITE:PS51194}. FT DOMAIN 2515 2779 MRNA cap 0-1 NS5-type MT. FT {ECO:0000259|PROSITE:PS51591}. FT DOMAIN 3043 3193 RdRp catalytic. {ECO:0000259|PROSITE: FT PS50507}. FT ACT_SITE 1547 1547 Charge relay system; for serine protease FT NS3 activity. {ECO:0000256|PIRSR: FT PIRSR003817-1}. FT ACT_SITE 1571 1571 Charge relay system; for serine protease FT NS3 activity. {ECO:0000256|PIRSR: FT PIRSR003817-1}. FT ACT_SITE 1631 1631 Charge relay system; for serine protease FT NS3 activity. {ECO:0000256|PIRSR: FT PIRSR003817-1}. SQ SEQUENCE 3417 AA; 378191 MW; 3B9B8F66DAA096F9 CRC64; MKNPKKKSGG FRIVNMLKRG VARVNPLGGL KRLPAGLLLG HGPIRMVLAI LAFLRFTAIK PSLGLINRWG TVGKKEAMEI IKKFKKDLAA MLRIINARKE RKRRGADTSI GIVGLLLTTA MAAEITRRGS AYYMYLDRSD AGKAISFATT LGVNKCHVQI MDLGHMCDAT MSYECPMLDE GVEPDDVDCW CNTTSTWVVY GTCHHKKGEA RRSRRAVTLP SHSTRKLQTR SQTWLESREY TKHLIKVENW IFRNPGFTLV AVAIAWLLGS STSQKVIYLV MILLIAPAYS IRCIGVSNRD FVEGMSGGTW VDVVLEHGGC VTVMAQDKPT VDIELVTTTV SNMAEVRSYC YEASISDMAS DSRCPTQGEA YLDKQSDTQY VCKRTLVDRG WGNGCGLFGK GSLVTCAKFT CSKKMTGKSI QPENLEYRIM LSVHGSQHSG MIVNDENRAK VEVTPNSPRA EATLGGFGSL GLDCEPRTGL DFSDLYYLTM NNKHWLVHKE WFHDIPLPWH AGADTGTPHW NNKEALVEFK DAHAKRQTVV VLGSQEGAVH TALAGALEAE MDGAKGRLFS GHLKCRLKMD KLRLKGVSYS LCTAAFTFTK VPAETLHGTV TVEVQYAGTD GPCKVPAQMA VDMQTLTPVG RLITANPVIT ESTENSKMML ELDPPFGDSY IVIGVGDKKI THHWHRSGST IGKAFEATVR GAKRMAVLGD TAWDFGSVGG VFNSLGKGIH QIFGAAFKSL FGGMSWFSQI LIGTLLVWLG LNTKNGSISL TCLALGGVMI FLSTAVSADV GCSVDFSKKE TRCGTGVFVY NDVEAWRDRY KYHPDSPRRL AAAVKQAWEE GICGISSVSR MENIMWKSVE GELNAILEEN GVQLTVVVGS VKNPMWRGPQ RLPVPVNELP HGWKAWGKSY FVRAAKTNNS FVVDGDTLKE CPLKHRAWNS FLVEDHGFGV FHTSVWLKVR EDYSLECDPA VIGTAVKGKE AAHSDLGYWI ESEKNDTWRL KRAHLIEMKT CEWPKSHTLW TDGVEESDLI IPKSLAGPLS HHNTREGYRT QVKGPWHSEE LEIRFEECPG TKVHVEETCG TRGPSLRSTT ASGRVIEEWC CRECTMPPLS FRAKDGCWYG MEIRPRKEPE SNLVRSMVTA GSTDHMDHFS LGVLVILLMV QEGLKKRMTT KIIMSTSMAV LVAMILGGFS MSDLAKLVIL MGATFAEMNT GGDVAHLALV AAFKVRPALL VSFIFRANWT PRESMLLALA SCLLQTAISA LEGDLMVLVN GFALAWLAIR AMAVPRTDNI ALAILAALTP LARGTLLVAW RAGLATCGGF MLLSLKGKGS VKKNLPFVAA LGLTAVRIVD PINVVGLLLL TRSGKRSWPP SEVLTAVGLI CALAGGFAKA DIEMAGPMAA VGLLIVSYVV SGKSVDMYIE RAGDITWEKD AEVTGNSPRL DVALDESGDF SLVEEDGPPM REIILKVVLM AICGMNPIAI PFAAGAWYVY VKTGKRSGAL WDVPAPKEVK KGETTDGVYR VMTRRLLGST QVGVGVMQEG VFHTMWHVTK GAALRSGEGR LDPYWGDVKQ DLVSYCGPWK LDAAWDGVSE VQLLAVPPGE RARNIQTLPG IFKTKDGDIG AVALDYPAGT SGSPILDKCG RVIGLYGNGV VIKNGSYVSA ITQGKREEET PVECFEPSML KKKQLTVLDL HPGAGKTRRV LPEIVREAIK KRLRTVILAP TRVVAAEMEE ALRGLPVRYM TTAVKVTHSG TEIVDLMCHA TFTSRLLQPI RVPNYNLYIM DEAHFTDPSS IAARGYISTR VEMGEAAAIF MTATPPGTRD AFPDSNSPIM DTEVEVPERA WSSGFDWVTD HSGKTVWFVP SVRNGNEIAA CLTKAGKRVI QLSRKTFETE FQKTKNQEWD FVITTDISEM GANFKADRVI DSRRCLKPVI LDGERVILAG PMPVTHASAA QRRGRIGRNP NKPGDEYMYG GGCAETDEDH AHWLEARMLL DNIYLQDGLI ASLYRPEADK VAAIEGEFKL RTEQRKTFVE LMKRGDLPVW LAYQVASAGI TYTDRRWCFD GTTNNTIMED SVPAEVWTKY GEKRVLKPRW MDARVCSDHA ALKSFKEFAA GKRGAALGVM EALGTLPGHM TERFQEAIDN LAVLMRAETG SRPYKAAAAQ LPETLETIML LGLLGTVSLG IFFVLMRNKG IGKMGFEMVT LGASAWLMWL SEIEPARIAC VLIVVFLLLV VLIPEPEKQR SPQDNQMAII IMVAVGLLGL ITANELGWLE RTKNDIAHLM GKREEGTTVG FSMDIDLRPA SAWAIYAALT TLITPAVQHA VTTSYNNYSL MAMATQAGVL FGMGKGMPFY AWDLGVPLLM MGCYSQLTPL TLIVAIILLV AHYMYLIPGL QAAAARAAQK RTAAGIMKNP VVDGIVVTDI DTMTIDPQVE KKMGQVLLIA VAVSSAVLLR TAWGWGEAGA LITAATSTLW EGSPNKYWNS STATSLCNIF RGSYLAGASL IYTVTRNAGL VKRRGGGTGE TLGEKWKARL NQMSALEFYS YKKSGITEVC REEARRALKD GVATGGHAVS RGSAKLRWLV ERGYLQPYGK VVDLGCGRGG WSYYAATIRK VQEVRGYTKG GPGHEEPMLV QSYGWNIIRL KSGVDVFHMA AESCDTLLCD IGESSSSPEV EETRTLRVLS MVGDWLEKRP GAFCIKVLCP YTSTMMETME RLQRRYGGGL VRVPLSRNST HEMYWVSGAK SNIIKSVSTT SQLLLGRMDG PRRPVKYEED VNLGSGTRAV ASCAEAPNMK VIGRRIERIR SEHAETWFFD ENHPYRTWAY HGSYEAPTQG SASSLVNGVV RLLSKPWDVV TGVTGIAMTD TTPYGQQRVF KEKVDTRVPD PQEGTRQVMN MVASWLWKEL GKRKRPRVCT KEEFINKVRS NAALGAIFEE EKEWKTAVEA VNDPRFWALV DKEREHHLRG ECHSCVYNMM GKREKKQGEF GKAKGSRAIW YMWLGARFLE FEALGFLNED HWMGRENSGG GVEGLGLQRL GYVLEEMSRA PGGKMYADDT AGWDTRISKF DLENEALITN QMDEGHRTLA LAVIKYTYQN KVVKVLRPAE GGKTVMDIIS RQDQRGSGQV VTYALNTFTN LVVQLIRNME AEEVLEMQDL WLLRKPEKVT RWLQSSGWDR LKRMAVSGDD CVVKPIDDRF AHALRFLNDM GKVRKDTQEW KPSTGWSNWE EVPFCSHHFN KLHLKDGRSI VVPCRHQDEL IGRARVSPGA GWSIRETACL AKSYAQMWQL LYFHRRDLRL MANAICSAVP VDWVPTGRTT WSIHGKGEWM TTEDMLMVWN RVWIEENDHM EDKTPVTKWT DIPYLGKRED LWCGSLIGHR PRTTWAENIK DTVSMVRRII GDEEKYMDYL STQVRYLGEE GSTPGVL //