ID C6L866_MIZYE Unreviewed; 604 AA. AC C6L866; DT 22-SEP-2009, integrated into UniProtKB/TrEMBL. DT 22-SEP-2009, sequence version 1. DT 05-JUN-2019, entry version 39. DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166}; DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166}; GN Name=PyEG {ECO:0000313|EMBL:BAH85844.1}; GN ORFNames=KP79_PYT21069 {ECO:0000313|EMBL:OWF45590.1}; OS Mizuhopecten yessoensis (Japanese scallop) (Patinopecten yessoensis). OC Eukaryota; Metazoa; Lophotrochozoa; Mollusca; Bivalvia; Pteriomorphia; OC Pectinoida; Pectinoidea; Pectinidae; Mizuhopecten. OX NCBI_TaxID=6573 {ECO:0000313|EMBL:BAH85844.1}; RN [1] {ECO:0000313|EMBL:BAH85844.1} RP NUCLEOTIDE SEQUENCE. RA Ojima T., Tsuchikawa M., Suzuki K., Tanaka H., Inoue A.; RT "Primary structure of a scallop cellulase."; RL Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|Proteomes:UP000242188} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=PY_sf001 {ECO:0000313|Proteomes:UP000242188}; RX PubMed=28812685; DOI=10.1038/s41559-017-0120; RA Wang S., Zhang J., Jiao W., Li J., Xun X., Sun Y., Guo X., Huan P., RA Dong B., Zhang L., Hu X., Sun X., Wang J., Zhao C., Wang Y., Wang D., RA Huang X., Wang R., Lv J., Li Y., Zhang Z., Liu B., Lu W., Hui Y., RA Liang J., Zhou Z., Hou R., Li X., Liu Y., Li H., Ning X., Lin Y., RA Zhao L., Xing Q., Dou J., Li Y., Mao J., Guo H., Dou H., Li T., Mu C., RA Jiang W., Fu Q., Fu X., Miao Y., Liu J., Yu Q., Li R., Liao H., Li X., RA Kong Y., Jiang Z., Chourrout D., Li R., Bao Z.; RT "Scallop genome provides insights into evolution of bilaterian RT karyotype and development."; RL Nat. Ecol. Evol. 1:120-120(2017). RN [3] {ECO:0000313|EMBL:OWF45590.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=PY_sf001 {ECO:0000313|EMBL:OWF45590.1}; RC TISSUE=Whole organism {ECO:0000313|EMBL:OWF45590.1}; RA Wang S., Zhang J., Jiao W., Li J., Xun X., Sun Y., Guo X., Huan P., RA Dong B., Zhang L., Hu X., Sun X., Wang J., Zhao C., Wang Y., Wang D., RA Huang X., Wang R., Lv J., Li Y., Zhang Z., Liu B., Lu W., Hui Y., RA Liang J., Zhou Z., Hou R., Li X., Liu Y., Li H., Ning X., Lin Y., RA Zhao L., Xing Q., Dou J., Li Y., Mao J., Guo H., Dou H., Li T., Mu C., RA Jiang W., Fu Q., Fu X., Miao Y., Liu J., Yu Q., Li R., Liao H., Li X., RA Kong Y., Jiang Z., Chourrout D., Li R., Bao Z.; RT "Scallop genome provides insights into evolution of bilaterian RT karyotype and development."; RL Nat. Ecol. Evol. 1:0-0(2017). CC -!- CATALYTIC ACTIVITY: CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; CC Evidence={ECO:0000256|RuleBase:RU361166}; CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) CC family. {ECO:0000256|PROSITE-ProRule:PRU10060, CC ECO:0000256|RuleBase:RU361166}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB513362; BAH85844.1; -; mRNA. DR EMBL; NEDP02004428; OWF45590.1; -; Genomic_DNA. DR CAZy; CBM2; Carbohydrate-Binding Module Family 2. DR CAZy; GH9; Glycoside Hydrolase Family 9. DR Proteomes; UP000242188; Unassembled WGS sequence. DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC. DR GO; GO:0030247; F:polysaccharide binding; IEA:InterPro. DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW. DR Gene3D; 1.50.10.10; -; 1. DR Gene3D; 2.60.40.290; -; 1. DR InterPro; IPR008928; 6-hairpin_glycosidase_sf. DR InterPro; IPR012341; 6hp_glycosidase-like_sf. DR InterPro; IPR001919; CBD2. DR InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf. DR InterPro; IPR012291; CBM2_carb-bd_dom_sf. DR InterPro; IPR001701; Glyco_hydro_9. DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS. DR Pfam; PF00553; CBM_2; 1. DR Pfam; PF00759; Glyco_hydro_9; 1. DR SUPFAM; SSF48208; SSF48208; 1. DR SUPFAM; SSF49384; SSF49384; 1. DR PROSITE; PS00698; GH9_3; 1. PE 2: Evidence at transcript level; KW Carbohydrate metabolism {ECO:0000256|PROSITE-ProRule:PRU10060, KW ECO:0000256|RuleBase:RU361166}; KW Cellulose degradation {ECO:0000256|RuleBase:RU361166}; KW Complete proteome {ECO:0000313|Proteomes:UP000242188}; KW Glycosidase {ECO:0000256|PROSITE-ProRule:PRU10060, KW ECO:0000256|RuleBase:RU361166}; KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU10060, KW ECO:0000256|RuleBase:RU361166}; KW Polysaccharide degradation {ECO:0000256|PROSITE-ProRule:PRU10060, KW ECO:0000256|RuleBase:RU361166}; KW Reference proteome {ECO:0000313|Proteomes:UP000242188}; KW Signal {ECO:0000256|RuleBase:RU361166}. FT SIGNAL 1 16 {ECO:0000256|RuleBase:RU361166}. FT CHAIN 17 604 Endoglucanase. {ECO:0000256|RuleBase: FT RU361166}. FT /FTId=PRO_5005125625. FT DOMAIN 20 116 CBM2. {ECO:0000259|Pfam:PF00553}. FT REGION 117 157 Disordered. {ECO:0000256|MobiDB-lite: FT C6L866}. FT COMPBIAS 120 157 Polar. {ECO:0000256|MobiDB-lite:C6L866}. FT ACT_SITE 562 562 {ECO:0000256|PROSITE-ProRule:PRU10060}. FT ACT_SITE 571 571 {ECO:0000256|PROSITE-ProRule:PRU10060}. SQ SEQUENCE 604 AA; 64192 MW; 504AEE6ECDB12DDC CRC64; MLRLLVLAVT VTLALGGATN VQITNEWPGG FQGTFTLHPD HALHGWKAHL QFSKPVDKVE IWKASVISKS NNNQEYVLQN VPGVGDIEGG SSLDVIFVAR ASGDHAGPVR VFLEGVDAPG SGSSSGSTGG TSGGGSSGTG ESSGGSGTPI SGGSSSVGSS TKYDYGNALG LSILFYDAQR SGRLPGNNPI PWRSNSATGD GSDVGTDLSG GWYDAGDLVK FNFPMASSAT ILAWGLSRWK DGYEAAGQLE MMYDCLKWPL DYFLKCWKPS QNVYYAQVGN GGTDHAVWGR PEDMHMSRPS YKVDAGKPGS DVAGETAAAL AAGSIVFKER DAGYSTKLLT AAKSLYEFAK NHKGIYSQSV PDAQSYYGST GYNDELCEAA AWLHKATQEA KYLQDAKGFY EADTSWALSW DDKKISCQLL LFEATKEAKY KANVESFVNS YKPGGGITYT PCGLAWRDQW GSLRYAANAA FVALMAAEDG IGGNDYKTFA LSQIDYILGD NRQHMSFEIG FGSNYPKQPH HRGSSCPGAN CGWNDYNSGG ANPHVLKGAL VGGPDQGDNY ADKRSDYTKN EVTCDYNAGF QSALAGLSSM ATRGQLPAAP NAKC //