ID C5QLA9_STAEP Unreviewed; 451 AA. AC C5QLA9; DT 01-SEP-2009, integrated into UniProtKB/TrEMBL. DT 01-SEP-2009, sequence version 1. DT 28-NOV-2012, entry version 22. DE RecName: Full=UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; DE EC=6.3.2.10; GN Name=murF; ORFNames=HMPREF0793_0015; OS Staphylococcus epidermidis M23864:W1. OC Bacteria; Firmicutes; Bacillales; Staphylococcus. OX NCBI_TaxID=525378; RN [1] RP NUCLEOTIDE SEQUENCE. RC STRAIN=M23864:W1; RA Qin X., Bachman B., Battles P., Bell A., Bess C., Bickham C., RA Chaboub L., Chen D., Coyle M., Deiros D.R., Dinh H., Forbes L., RA Fowler G., Francisco L., Fu Q., Gubbala S., Hale W., Han Y., RA Hemphill L., Highlander S.K., Hirani K., Hogues M., Jackson L., RA Jakkamsetti A., Javaid M., Jiang H., Korchina V., Kovar C., Lara F., RA Lee S., Mata R., Mathew T., Moen C., Morales K., Munidasa M., RA Nazareth L., Ngo R., Nguyen L., Okwuonu G., Ongeri F., Patil S., RA Petrosino J., Pham C., Pham P., Pu L.-L., Puazo M., Raj R., Reid J., RA Rouhana J., Saada N., Shang Y., Simmons D., Thornton R., Warren J., RA Weissenberger G., Zhang J., Zhang L., Zhou C., Zhu D., Muzny D., RA Worley K., Gibbs R.; RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Involved in cell wall formation. Catalyzes the final CC step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the CC precursor of murein (By similarity). CC -!- CATALYTIC ACTIVITY: ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D- CC glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N- CC acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D- CC alanine. CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the MurCDEF family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; ACJB01000001; EES42326.1; -; Genomic_DNA. DR PATRIC; 31399002; VBIStaEpi14170_2370. DR UniPathway; UPA00219; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0047480; F:UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity; IEA:EC. DR GO; GO:0008766; F:UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; IEA:InterPro. DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW. DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW. DR GO; GO:0007047; P:cellular cell wall organization; IEA:UniProtKB-KW. DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW. DR Gene3D; 3.90.190.20; Mur_ligase_C; 1. DR Gene3D; 3.40.1190.10; Mur_ligase_cen; 1. DR InterPro; IPR004101; Mur_ligase_C. DR InterPro; IPR013221; Mur_ligase_cen. DR InterPro; IPR000713; Mur_ligase_N. DR InterPro; IPR005863; UDP-N-AcMur-pentapeptide_synth. DR PANTHER; PTHR23135:SF3; PTHR23135:SF3; 1. DR Pfam; PF01225; Mur_ligase; 1. DR Pfam; PF02875; Mur_ligase_C; 1. DR Pfam; PF08245; Mur_ligase_M; 1. DR SUPFAM; SSF53244; Mur_ligase_C; 1. DR SUPFAM; SSF53623; Mur_ligase_cen; 1. DR TIGRFAMs; TIGR01143; murF; 1. PE 3: Inferred from homology; KW ATP-binding; Cell cycle; Cell division; Cell shape; KW Cell wall biogenesis/degradation; Ligase; Nucleotide-binding; KW Peptidoglycan synthesis. SQ SEQUENCE 451 AA; 49744 MW; 1D88E5B68457B276 CRC64; MINVTLQQIQ NWISCEIDEQ YLSRSIKGVT IDSRAIEKDM LFIPFKGENV DGHRFIDQAL KDGAVASFSE KGTELSEDVE GPIIWVEDTL QALQDLAKAY LKHVNPKVIA VTGSNGKTTT KDMIESVLTT EFKVKKTQGN YNNEIGMPLT ILDLDEDTEI SILEMGMSGF HEIELLSTIA EPDIAVITNI GESHMQDLGS REGIAKAKSE ITIGLKDNGT FIYDGDEPLL TPHVKQIQQA KLISVGLGDA NSLVCHVDDV ETEGIGFTIN NDESYVLPIL GIHNMKNAAI AIAVGHELGL PYATIKENIR HVQLTGMRME RHQTDDNVTV INDAYNASPT SMKAAIDTLS SMEGRKILIL ADVLELGTNS QQMHEEVGTY LDGKSIDILF TYGTEAEYIF NTGQSYVETA KHFDDKSELI KELKAIVKEN DKILVKGSRG MKLEEVVEAL I //